ANTI-PHO-H2AX (SER139) 200UG

货号： 07-164      产品名称： ANTI-PHO-H2AX (SER139) 200UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-H2A.X (Ser139) Antibody
Recommended Replacement for: 07-164 is a recommended replacement for AB3369
Species Reactivity Key Applications Host Format Antibody Type
Vrt, H  IP, WB, ChIP-seq Rabbit Purified Polyclonal Antibody
Description:
Anti-phospho-H2A.X (Ser139) Antibody
Replaces:
07-164 is a recommended replacement for AB3369
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Trade Name:
Upstate (Millipore)
Specificity:
Histone H2A.X phosphorylated at Ser139.
Molecular Weight:
15 kDa
Epitope:
a.a. 134-142
Immunogen:
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
Modifications:
Phosphorylation
Isotype:
IgG
Background Information:
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
Species Reactivity:
Vertebrates
Human
Species Reactivity Note:
Human; broad species cross-reactivity expected based on conservation of sequence homology.
Application Notes:
Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
Control:
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
Quality Assurance:
Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
Purification Method:
Protein A chromatography
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
P16104
Entrez Gene Number:
NP_002096
Gene Symbol:
HIST2H2AC
H2A-GL101
H2AFQ
H2A/r
H2A/q
MGC74460
H2A
Alternate Names:
H2A histone family, member Q
histone 2, H2ac
histone IIa
histone cluster 2, H2ac
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunoprecipitation
Western Blotting
ChIP-seq
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The link -20℃