HEK-Blue hTLR4 cells
货号： hkb-htlr4 产品名称： HEK-Blue hTLR4 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ hTLR4
HEK-Blue™-hTLR4 cells were obtained by co-transfection of the human TLR4, MD-2 and CD14 co-receptor genes, and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of an IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR4 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR4 cells is HEK-Blue™ Null2 cells.
Contents update notification
August, 5th 2013 – Please note cells now come with one pouch of HEK-Blue™ Detection for analysis purposes.
Antibiotic resistance: blasticidin, hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
