Screen Quest Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10×10 plates*
货号： 36332 产品名称： Screen Quest Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10×10 plates* 品牌： AAT 规格： 1 kit 二周到货 生化试验
Screen Quest� Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10×10 plates*
Cat # 36332
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 530
Em (nm) 590
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest� Rhod-4 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Rhod-4 NW which can cross cell membrane. Rhod-4 is the brightest red calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Quest Rhod-4� are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Rhod-4. The characteristics of its long wavelength, high sensitivity, and >250 times fluorescence increases (when it forms complexes with calcium) make Quest Rhod-4� an ideal indicator for measurement of cellular calcium. This Screen Quest Rhod-4 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.
Detailed Information: Protocol References MSDS Spectrum
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
