HEK-Blue CD40L cells
货号： hkb-cd40 产品名称： HEK-Blue CD40L cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ CD40L Cells
CD40L Sensor Cells
HEK-Blue™ CD40L cells can serve to measure the bioactivity of CD40L through the secretion of embryonic alkaline phosphatase (SEAP) upon NF-κB activation following CD40 stimulation. These cells were generated by stable transfection of HEK293 cells with the human CD40 gene and an NF-κB-inducible SEAP construct. Binding of CD40L to its receptor CD40 triggers a signaling cascade leading to the activation of NF-κB and the subsequent production of SEAP. CD40L-CD40 interaction can be monitored by assessing the levels of SEAP using QUANTI-Blue™. HEK293 cells express endogenously the receptors for the cytokines IL-1β and TNF-α which share a common signaling pathway with CD40L. Consequently, HEK-Blue™ CD40L cells also respond to IL-1β and TNF-α. IL-1β- and TNF-α-mediated SEAP production can be blocked using neutralizing antibodies, such as anti-hIL-1β-IgA and anti-hTNF-α-IgA respectively.
HEK-Blue™ CD40L cells are available in a kit containing anti-hCD40L-IgA.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Screen for molecules that interfere with CD40L-CD40 cross-talk
Detection range for human CD40L: 5 ng – 1 μg/ml
