HEK-Blue IL-6 kit
货号： hkb-il6-kit 产品名称： HEK-Blue IL-6 kit 品牌： Invivogen 规格： 1 kit 二周到货 生化试验
HEK-Blue™ IL-6 Cells
IL-6 Reporter Cells
HEK-Blue™ IL-6 cells allow the detection of bioactive IL-6 by monitoring the activation of the STAT-3 pathway. These cells were generated by stable transfection of HEK293 cells with the human IL-6R gene and a STAT3-inducible SEAP reporter gene. Upon IL-6 stimulation, HEK-Blue™ IL-6 cells trigger the activation of STAT3 and the subsequent secretion of SEAP. SEAP can be readily monitored using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-6 cells with recombinant human IL-6 can be blocked by hIL-6-IgA, a neutralizing monoclonal antibody of the IgA isotype.
HEK-Blue™ IL-6 cells are also available in a kit containing hIL-6-IgA.
Antibiotic resistance: Hygromycin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-6
Detection range for human IL-6: 0.5 – 50 ng/
