HEK-Blue TNF-α/IL-1β cells
货号： hkb-tnfil1 产品名称： HEK-Blue TNF-α/IL-1β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ TNF-α/IL-1β Cells
TNF-α and IL-1β Reporter Cells
HEK-Blue™ TNF-α/IL-1β cells are designed to detect bioactive human and murine TNF-α and IL-1β by monitoring the activation of the NF-κB pathway. These cells were generated by stable transfection of HEK293 cells with an NF-κB/AP-1-inducible SEAP reporter gene. Secretion of SEAP by HEK-Blue™ TNF-α/IL-1β cells is specifically induced by TNF-α and IL-1β. SEAP levels can be readily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ TNF-α/IL-1β cells with recombinant human TNF-α or IL-1β can be blocked by the neutralizing monoclonal anti-hTNF-α-IgA or anti-hIL-1β-IgA antibody, respectively.
HEK-Blue™ TNF-α/IL-1β cells are also available in a kit containing anti-hTNF-α-IgA and anti-hIL-1β-IgA.
Antibiotic resistance: Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human and murine TNF-α and IL-1β(α)
• Detection range for human TNF-α: 0.5 ng – 1 μg/ml
• Detection range for human IL-1β: 0.2 – 100 ng/ml
