HEK-Blue TGF-β cells
货号： hkb-tgfb 产品名称： HEK-Blue TGF-β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ TGF-β Cells
TGF-β Sensor Cells
HEK-Blue™ TGF-β cells allow the detection of bioactive TGF-β by monitoring the activation of the TGF-β/Smad pathway. They were generated by stable transfection of HEK293 cells with the human TGFBRI, Smad3 and Smad4 genes. They further express a Smad3/4-binding elements (SBE)-inducible SEAP reporter gene. Stimulation of HEK-Blue™ TGF-β cells with TGF-β induces the activation of the TGF-β/Smad signaling pathway leading to the formation of a Smad3/Smad4 complex. The heterocomplex enters the nucleus and binds SBE sites inducing the production of SEAP. The quantity of SEAP secreted in the supernatant can be easily assessed using QUANTI-Blue™. TGF-β-mediated SEAP production can be blocked using a neutralizing antibody, such as anti-hTGFβ-IgA.
HEK-Blue™TGF-β cells are available in a kit containing anti-hTGFβ-IgA.
Antibiotic resistance: blasticidin, hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human TGF-β
Detection range for human TGF-β: 0.1 – 10 ng/ml
