HEK-Blue IL-4/IL-13 cells
货号： hkb-stat6 产品名称： HEK-Blue IL-4/IL-13 cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IL-4/IL-13 Cells
IL-4 and IL-13 Sensor Cells
HEK-Blue™ IL-4/IL-13 cells allow the detection of bioactive IL-4 and IL-13 by monitoring the activation of the STAT-6 pathway. These cells were generated by stable transfection of HEK293 cells with the human STAT6 gene to obtain a fully active STAT6 pathway. The other genes of the pathway are naturally expressed in sufficient amounts. The cells were further transfected with a STAT6-inducible SEAP reporter gene. HEK-Blue™ IL-4/IL-13 cells produce SEAP in response to IL-4 or IL-13 stimulation and to a lower extent IFN-α. The levels of SEAP secreted in the supernatant can be easily determined using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-4/IL-13 cells with human recombinant IL-4 or IL-13 can be blocked by the neutralizing monoclonal hIL-4-IgA and hIL-13-IgA antibodies, respectively.
HEK-Blue™ IL-4/IL-13 cells are also available in a kit containing anti-hIL-4-IgA and anti-hIL-13-IgA.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-4 and human/murine IL-13
• Detection range for human IL-4: 0.5 – 100 ng/ml
• Detection range for human IL-13: 5 – 1000 ng/ml
