HEK-Blue IL-33/IL-1β cells
货号： hkb-il33 产品名称： HEK-Blue IL-33/IL-1β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IL-33/IL-1β Cells
IL-33/IL-1β Reporter Cells
HEK-Blue™ IL-33/IL-1β cells are designed to detect bioactive IL-33 and IL-1β (and IL-1α) by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK-Blue™ IL-1β cells with the IL1RL1 gene. They express a SEAP repor ter gene under the control of the IFN-β minimal promoter fused to five NF-κB and five AP-1 binding sites. Stimulation of HEK-Blue™ IL-33/IL-1β cells with human IL-33 or IL-1β, but not with IL-18 nor TNF-α, activates the NF-κB and AP-1 pathways triggering the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IL-33/IL-1β cells with recombinant human IL-1β can be blocked by using the neutralizing antibody anti-hIL-1β-IgA.
Antibiotic resistance: blasticidin, hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-33 and human/murine IL-1β (α)
• Detection range for human IL-33: 0.5 – 100 ng/ml
• Detection range for human IL-1β: 0.5 – 100 ng/ml
