Phospho-H2AX pSer140
货号： MA12022 产品名称： Phospho-H2AX pSer140 品牌： Pierce 规格： 100 μg
puno1ha-hikka pUNO1-hIKKA-HA3x 品牌 Invivogen
pUNO1-hIKKA-HA3x
货号： puno1ha-hikka 产品名称： pUNO1-hIKKA-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
IKKα-HA
Coding sequence in expression plasmid
Alias: IKBKA, CHUK
Inhibitor of Kappa light polypeptide gene enhancer in B-cells, Kinase Alpha, HA-tagged
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human IKKα-HA (pUNO1-hIKKA-HA3x)
puno1ha-hikkb pUNO1-hIKBKB-HA3x 品牌 Invivogen
pUNO1-hIKBKB-HA3x
货号： puno1ha-hikkb 产品名称： pUNO1-hIKBKB-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
IKKβ-HA
Coding sequence in expression plasmid
Alias: IKBKB
Inhibitor of Kappa light polypeptide gene enhancer in B-cells, Kinase Beta, HA-tagged
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human IKKβ-HA (pUNO1-hIKBKB-HA3x)
Genbank: NM_001556
ORF Size: 2364 bp
Subclone: AgeI – NheI
Mouse IKKβ-HA (pUNO1-mIKBKB-HA3x)
Genbank: NM_010546
ORF Size: 2309 bp
Subclone: AgeI – NheI
puno1ha-htlr1 pUNO1-hTLR01-HA3x 品牌 Invivogen
pUNO1-hTLR01-HA3x
货号： puno1ha-htlr1 产品名称： pUNO1-hTLR01-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
TLR1-HA
Coding sequence in expression plasmid
Alias: CD281
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human TLR1-HA (pUNO1-hTLR01-HA3x)
ORF Size: 2454 bp
Subclone: AgeI – NheI
Mouse TLR1-HA (pUNO1-mTLR01-HA3x)
ORF Size: 2385 bp
Subclone: BspHI – NheI
puno1ha-htlr3 pUNO1-hTLR3-HA3x 品牌 Invivogen
pUNO1-hTLR3-HA3x
货号： puno1ha-htlr3 产品名称： pUNO1-hTLR3-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
TLR3-HA
Coding sequence in expression plasmid
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human TLR3-HA (pUNO1-hTLR03-HA3x)
ORF Size: 2787 bp
Subclone: AgeI – NheI
Mouse TLR3-HA (pUNO1-mTLR03-HA3x)
ORF Size: 2811 bp
Subclone: AgeI – NheI
puno1ha-htlr4a pUNO1-hTLR04a-HA3x 品牌 Invivogen
pUNO1-hTLR04a-HA3x
货号： puno1ha-htlr4a 产品名称： pUNO1-hTLR04a-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
TLR4-HA
Coding sequence in expression plasmid
Alias: CD284
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human TLR4a-HA (pUNO1-hTLR04a-HA3x)
ORF Size: 2613 bp
Subclone: AgeI – NheI
Mouse TLR4-HA (pUNO1-mTLR04-HA3x)
ORF Size: 2601 bp
Subclone: AgeI – NheI
puno1ha-htlr5 pUNO1-hTLR05-HA3x 品牌 Invivogen
pUNO1-hTLR05-HA3x
货号： puno1ha-htlr5 产品名称： pUNO1-hTLR05-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
TLR5-HA
Coding sequence in expression plasmid
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human TLR5-HA (pUNO1-hTLR05-HA3x)
ORF Size: 2670 bp
Subclone: NcoI – NheI
Mouse TLR5-HA (pUNO1-mTLR05-HA3x)
ORF Size: 2577 bp
Subclone: NcoI – NheI
puno1ha-htlr7 pUNO1-hTLR07-HA3x 品牌 Invivogen
pUNO1-hTLR07-HA3x
货号： puno1ha-htlr7 产品名称： pUNO1-hTLR07-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
TLR7-HA
Coding sequence in expression plasmid
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human TLR7-HA (pUNO1-hTLR07-HA3x)
ORF Size: 3243 bp
Subclone: AgeI – NheI
Mouse TLR7-HA (pUNO1-mTLR07-HA3x)
ORF Size: 3246 bp
Subclone: AgeI – NheI
puno1ha-htlr8b pUNO1-hTLR08b-HA3x 品牌 Invivogen
pUNO1-hTLR08b-HA3x
货号： puno1ha-htlr8b 产品名称： pUNO1-hTLR08b-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
TLR8-HA
Coding sequence in expression plasmid
Alias: CD288
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human TLR8-HA (pUNO1-hTLR08-HA3x)
ORF Size: 3220 bp
Subclone: AgeI – NheI
Mouse TLR8-HA (pUNO1-mTLR08-HA3x)
ORF Size: 3096 bp
Subclone: AgeI – NheI
puno1ha-mikkb pUNO1-mIKBKB-HA3x 品牌 Invivogen
pUNO1-mIKBKB-HA3x
货号： puno1ha-mikkb 产品名称： pUNO1-mIKBKB-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
IKKβ-HA
Coding sequence in expression plasmid
Alias: IKBKB
Inhibitor of Kappa light polypeptide gene enhancer in B-cells, Kinase Beta, HA-tagged
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human IKKβ-HA (pUNO1-hIKBKB-HA3x)
Genbank: NM_001556
ORF Size: 2364 bp
Subclone: AgeI – NheI
Mouse IKKβ-HA (pUNO1-mIKBKB-HA3x)
Genbank: NM_010546
ORF Size: 2309 bp
Subclone: AgeI – NheI
puno1ha-mtlr4 pUNO1-mTLR4-HA3x 品牌 Invivogen
pUNO1-mTLR4-HA3x
货号： puno1ha-mtlr4 产品名称： pUNO1-mTLR4-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
TLR4-HA
Coding sequence in expression plasmid
Alias: CD284
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human TLR4a-HA (pUNO1-hTLR04a-HA3x)
ORF Size: 2613 bp
Subclone: AgeI – NheI
Mouse TLR4-HA (pUNO1-mTLR04-HA3x)
ORF Size: 2601 bp
Subclone: AgeI – NheI
puno1ha-mtlr7 pUNO1-mTLR7-HA3x 品牌 Invivogen
pUNO1-mTLR7-HA3x
货号： puno1ha-mtlr7 产品名称： pUNO1-mTLR7-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
TLR7-HA
Coding sequence in expression plasmid
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human TLR7-HA (pUNO1-hTLR07-HA3x)
ORF Size: 3243 bp
Subclone: AgeI – NheI
Mouse TLR7-HA (pUNO1-mTLR07-HA3x)
ORF Size: 3246 bp
Subclone: AgeI – NheI
punoha-hnemo pUNO-hNEMO-HA3x 品牌 Invivogen
pUNO-hNEMO-HA3x
货号： punoha-hnemo 产品名称： pUNO-hNEMO-HA3x 品牌： Invivogen 规格： 1 disk 三周到货 生化试验
NEMO-HA
Coding sequence in expression plasmid
Alias: IKBKG
HA-tagged
NF-kappaB Essential Modulator.
Inhibitor of Kappa light polypeptide gene enhancer in B-cells, Kinase Gamma
The gene has been fused at the 3’end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.
This gene is available in pUNO1-HA expression plasmid selectable with Blasticidin.
Human NEMO-HA (pUNO1-hNEMO-HA3x)
TP-004-HAX Kit PV+ HRP-AEC (5) 4mL 品牌 NeoMarkers
Kit PV+ HRP-AEC (5) 4mL
货号： TP-004-HAX 产品名称： Kit PV+ HRP-AEC (5) 4mL 品牌： NeoMarkers 规格： ***
ABT65 Anti-HAX-1 品牌 Millipore
Anti-HAX-1
货号： ABT65 产品名称： Anti-HAX-1 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-HAX-1
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-HAX-1 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes HAX-1 at the domains involved in HCLS1-, CASP9-, and GNA13-binding.
Molecular Weight:
~35 kDa observed.
HAX-1 has been shown to migrate as a ~34-36 kDa band in WB (Ortiz, D.F., et al. (2004). JBC. 279(31):32761-32770).
Epitope:
HCLS1-, CASP9-, and GNA13-binding domains
Immunogen:
KLH-conjugated linear peptide corresponding to the domain of human HAX-1 that is involved in HCLS1-, CASP9-, and GNA13-bindings.
Background Information:
HAX-1 (HS1-associated protein X-1) is a mitochondrial anti-apoptotic protein and is thought to be regulated by Omi/HtrA2. HAX-1 has been found to associate with BSEP, MDR1 and MDR2 ABC-transporters and interacts with cortactin to play a role in BSEP internalization from the apical membrane. HAX-1 and alphavbeta6 are found to be highly expressed in certain oral cancers and work together to enhance tumor invasion and progression. High expression of HAX-1 in many other cancer tissues shows that HAX-1 is important in neoplastic transformation.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Human, Mouse, and Rat.
Application Notes:
Western Blot Analysis: 0.1 µg/mL from a representative lot detected HAX-1 in 10 µg of HeLa cell lysate and rat liver tissue lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HAX-1 in NIH/3T3 and HeLa cells.
Control:
Jurkat cell lysate
Quality Assurance:
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected HAX-1 in 10 µg of Jurkat cell lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O00165
Entrez Gene Number:
NP_006109
Gene Symbol:
HAX1
HS1BP1
Alternate Names:
HCLS1-associated protein X-1
HS1-associating protein X-1
HAX-1
HS1-binding protein 1
HSP1BP-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
UniProt Summary:
FUNCTION: P 2-8℃
PA114197 Phospho-Histone H2AX pSer139 品牌 Pierce
Phospho-Histone H2AX pSer139
货号： PA114197 产品名称： Phospho-Histone H2AX pSer139 品牌： Pierce 规格： 100 μg
PA114198 Histone H2AX 品牌 Pierce
Histone H2AX
货号： PA114198 产品名称： Histone H2AX 品牌： Pierce 规格： 200 g
07-164 ANTI-PHO-H2AX (SER139) 200UG 品牌 Millipore
ANTI-PHO-H2AX (SER139) 200UG
货号： 07-164 产品名称： ANTI-PHO-H2AX (SER139) 200UG 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-phospho-H2A.X (Ser139) Antibody
Recommended Replacement for: 07-164 is a recommended replacement for AB3369
Species Reactivity Key Applications Host Format Antibody Type
Vrt, H IP, WB, ChIP-seq Rabbit Purified Polyclonal Antibody
Description:
Anti-phospho-H2A.X (Ser139) Antibody
Replaces:
07-164 is a recommended replacement for AB3369
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Histone H2A.X phosphorylated at Ser139.
Molecular Weight:
15 kDa
Epitope:
a.a. 134-142
Immunogen:
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.
Modifications:
Phosphorylation
Isotype:
IgG
Background Information:
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
Species Reactivity:
Vertebrates
Human
Species Reactivity Note:
Human; broad species cross-reactivity expected based on conservation of sequence homology.
Application Notes:
Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
Control:
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
Quality Assurance:
Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
Purification Method:
Protein A chromatography
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
UniProt Number:
P16104
Entrez Gene Number:
NP_002096
Gene Symbol:
HIST2H2AC
H2A-GL101
H2AFQ
H2A/r
H2A/q
MGC74460
H2A
Alternate Names:
H2A histone family, member Q
histone 2, H2ac
histone IIa
histone cluster 2, H2ac
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunoprecipitation
Western Blotting
ChIP-seq
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The link -20℃
AB10021 ANTI-Chicken Specific H2AX 品牌 Millipore
ANTI-Chicken Specific H2AX
货号： AB10021 产品名称： ANTI-Chicken Specific H2AX 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-Histone H2A.X,Chicken Antibody (AB10021)
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PRODUCT FAMILY INFORMATION
Histone H2A Antibodies, Proteins and Kits
Millipore’s Histone H2A antibodies demonstrate specificity against linker histone H2A and its modifications. Millipore’s Histone H2A specific antibodies, proteins, and kits are presented below.
EMD Millipore’s Histone H2A antibody demonstrates specificity against linker histone H2A. See below for data and references for EMD Millipore’s Histone H2A antibodies, proteins and kits. All EMD Millipore antibodies are based on the expertise of Upstate & Chemicon.
» More…
Species Reactivity Key Applications Host Format Antibody Type
Ch WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Histone H2A.X Antibody,Chicken
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Chicken H2A.X, Mr 15 kDa.
Molecular Weight:
15 kDa
Epitope:
a.a. 125-139
Immunogen:
The immunogen used was a synthetic peptide TFKSTGVPKEQPGSQ-C, corresponding to a.a. 125-139 of chicken H2A.X, coupled to KLH.
Background Information:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.
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Species Reactivity:
Chicken
Species Reactivity Note:
Chicken. Does not recognize human H2A.X. Cross-reactivity with other species has not been tested..
Control:
Chicken heart tissue lysate
Quality Assurance:
routinely evaluated by immunoblot on lysates from chicken heart tissue
Purification Method:
Peptide affinity chromatography
Presentation:
Purified from rabbit serum by affinity chromatography. Supplied at 1 mg/ml in PBS with 0.1% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
UniProt Number:
P16104
Entrez Gene Number:
NP_002096
Gene Symbol:
H2AFX
H2A.X
Alternate Names:
H2AFX
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the cons 2-8℃
AB10022 ANTI-Human Specific H2AX 品牌 Millipore
ANTI-Human Specific H2AX
货号： AB10022 产品名称： ANTI-Human Specific H2AX 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-Histone H2A.X, Human Antibody (AB10022)
Close
PRODUCT FAMILY INFORMATION
Histone H2A Antibodies, Proteins and Kits
Millipore’s Histone H2A antibodies demonstrate specificity against linker histone H2A and its modifications. Millipore’s Histone H2A specific antibodies, proteins, and kits are presented below.
EMD Millipore’s Histone H2A antibody demonstrates specificity against linker histone H2A. See below for data and references for EMD Millipore’s Histone H2A antibodies, proteins and kits. All EMD Millipore antibodies are based on the expertise of Upstate & Chemicon.
» More…
Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Histone H2A.X Antibody, Human
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Human H2A.X, Mr 15 kDa.
Molecular Weight:
15 kDa
Epitope:
a.a. 121-139
Immunogen:
The immunogen used was a synthetic peptide TSATVGPKAPSGGKKATQA-C, corresponding to a.a. 121-139 of human H2A.X, coupled to KLH.
Background Information:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.
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Species Reactivity:
Human
Species Reactivity Note:
Human. Does not recognize chicken H2A.X. Cross-reactivity with other species has not been tested.
Control:
HeLa acid extracts
Quality Assurance:
routinely evaluated by immunoblot on lysates from HeLa cells
Purification Method:
Peptide affinity chromatography
Presentation:
Purified from rabbit serum by affinity chromatography. Supplied at 1 mg/mL in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Azide
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
UniProt Number:
P16104
Entrez Gene Number:
NM_002105.2
Gene Symbol:
H2AFX
H2A.X
Alternate Names:
H2AFX
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the cons 2-8℃