ANTI-DNMT3A2 Antibody (C-Terminus)

货号： 07-2050      产品名称： ANTI-DNMT3A2 Antibody (C-Terminus)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-DNMT3A2 Antibody
Description:
Anti-DNMT3A2 Antibody
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Specificity:
The antibody recognizes isoform 2 of human DNMT3A. Additional lower molecular weight bands are detected in some samples.
Molecular Weight:
~80 kDa
Epitope:
C-terminus
Immunogen:
The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human DNMT3A2.
Modifications:
Methylation
Background Information:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3 is a developmentally regulated DNA methyltransferase. DNMT3A2 is a second isoform of DNMT3 that is expressed from an alternative promoter. DNMT3A2 localizes to euchromatin and is thought to function in de novo methylation, rather than maintenance methylation. Binding of DNMT3L (DNMT3-like protein) to DNMT3A2 promotes a dramatic reorganization of DNMT3A2 subunits and leads to the formation of specific complexes with enhanced DNA methyltransferase activity. DNMT3A2 targets endogenous DNMT3L to embryonic stem cell chromatin and induces regional DNA methylation.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Other homologies: Predicted to react with Mouse and Rat based on 100% sequence homology
Application Notes:
ELISA:
This antibody was shown by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
This antibody detects DMNT3A2 in the nuclei of NIH/3T3, HeLa, and A431 cells.
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Control:
L6, NIH/3T3, HeLa, or A431 cells.
Quality Assurance:
Western Blot Analysis: 1:1,000 dilution of this antibody detected DNMT3A2 on 10 µg of L6 lysate.
Purification Method:
Protein A chromatography
Presentation:
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q9Y6K1
Entrez Gene Number:
NP_715640
Gene Symbol:
DNMT3A2
Dnmt3a
EC 2.1.1.37
M.HsaIIIA
OTTHUMP00000120005
OTTHUMP00000120006
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Alternate Names:
DNA (cytosine-5-)-methyltransferase 3 alpha
DNA MTase HsaIIIA
DNA cytosine methyltransferase 3 alpha
DNA cytosine methyltransferase 3A2
DNA methyltransferase HsaIIIA
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
ELISA
Western Blotting
Entrez Gene Summary:
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to 2°C – 8°C