HEK-Blue IL-18/1L-1β cells
货号： hkb-il18 产品名称： HEK-Blue IL-18/1L-1β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IL-18/IL-1β Cells
IL-18/IL-1β Sensor Cells
HEK-Blue™ IL-18/IL-1β cells are designed to detect bioactive IL-18 and IL-1β by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK-Blue™ IL-1β cells with the gene encoding the IL-18 receptor accessory protein (IL-18RAP). They express a NF-κB/AP-1 inducible SEAP reporter gene.
HEK-Blue™ IL-18/IL-1β cells are useful to monitor IL-18 and IL-1β in inflammasome activation studies. These cells detect the presence of IL-18 and IL-1β in samples which triggers the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™. Stimulation of HEK-Blue™ IL-18/IL-1β cells with recombinant human IL-1β or IL-18 can be blocked by the neutralizing antibodies anti-hIL-1β-IgA and anti-hIL-18-IgA, respectively. These antibodies allow to determine the respective contribution of each cytokine in inflammasome studies.
HEK-Blue™ IL-18/IL-1β cells are available in a kit containing anti-hIL-1β-IgA and anti-hIL-18-IgA.
Antibiotic resistance: blasticidin, hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human IL-18 and human/murine IL-1β (α)
• Detection range for human IL-18: 0.5 – 100 ng/ml
• Detection range for human IL-1β: 0.5 – 100 ng/ml
