HEK-Blue IL-1β cells
货号： hkb-il1b 产品名称： HEK-Blue IL-1β cells 品牌： Invivogen 规格： 5-7 x 10e6 cells 二周到货 生化试验
HEK-Blue™ IL-1β Cells
IL-1β Sensor cells
HEK-Blue™ IL-1β cells allow to detect bioactive IL-1β by monitoring the activation of the NF-κB and AP-1 pathways. They derive from HEK-Blue™ TNF-α/IL-1β cells in which the TNF-α response has been blocked. Therefore, HEK-Blue™ IL-1β cells respond specifically to IL-1β. They express a NF-κB/AP-1-inducible SEAP reporter gene. Binding of IL-1β to its receptor IL-1R on the surface of HEK-Blue™ IL-1β cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.
HEK-Blue™ IL-1β cells are useful to monitor IL-1β in inflammasome activation studies. These cells detect the presence of IL-1β in samples which triggers the production of SEAP. Levels of SEAP can be easily monitored using QUANTI-Blue™. Stimulation of HEK-Blue™ IL-1β cells with recombinant human IL-1β can be blocked by the neutralizing monoclonal anti-hIL-1β-IgA antibody.
HEK-Blue™ IL-1β cells are also available in a kit containing anti-hIL-1β-IgA.
Antibiotic resistance: hygromycin B, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-Glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects human and murine IL-1β (α)
• Detection range for human IL-1β: 100 pg – 100 ng/ml
• Detection range for murine IL-1β: 10 ng – 1 µg/ml
