Screen Quest Luminometric Calcium Assay Kit *10X10 Plates*
货号： 36306 产品名称： Screen Quest Luminometric Calcium Assay Kit *10X10 Plates* 品牌： AAT 规格： 100 Plates 二周到货 生化试验
Screen Quest� Luminometric Calcium Assay Kit *10X10 Plates*
Cat # 36306
Storage F/D/L (see storage codes below)
Unit Size 100 Plates
Ex (nm) 437
Em (nm) 466
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine朾ased kit is much more sensitive than the fluorescence-based calcium assay kits (such as Fluo-4, Fluo-3, Calcium-3 and Calcium-4). This kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring of intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
