标签归档：pde

V1361   PDE-Glo Phosphodiesterase Assay   品牌 Promega

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PDE-Glo Phosphodiesterase Assay

货号： V1361      产品名称： PDE-Glo Phosphodiesterase Assay   品牌： Promega 规格： 1,000 assays 二周到货 生化试验

说明
    PDE-Glo™ Phosphodiesterase Assay( PDE-Glo™ 磷酸二酯酶检测试剂盒) 是一种发光的、高通量筛选(HTS) 的方法，用来对纯化来源的环状核苷磷酸二酯酶活性进行检测。环状核苷磷酸二酯酶(PDEs) 由于其水解能力，调控了第二信使信号分子cAMP 和cGMP的水平，因此参与了无数的细胞进程。
    PDEs 选择性抑制分子的存在可帮其作为工具用于研究环状核苷信号，并为研究PDEs 在细胞和组织病理方面的作用创造了条件。PDE-Glo™ 磷酸二酯酶检测试剂盒可从化合物文库中筛选和鉴定候选前导化合物。这种检测方法被设计成用于384 孔板，但检测的体系可以很容易的调整为96 或1536 孔平板。PDE-Glo™ 磷酸二酯酶检测试剂盒经过优化可同时检测cAMP和cGMP依赖的磷酸二酯酶。在PDEs 反应完成后，检测所需要的时间从开始到结束不到一小时。
特点  • 多功能
• 可检测cAMP 和cGMP 磷酸二酯酶。
• 灵敏
• 优良的信号: 背景比。
• 可调整至用1536 孔板模式。
• 快速、易用
• 检测可在一小时内完成。
• 均质。
• 行之有效的发光技术
• Ultra-Glo™ 萤光素酶驱动。
• 无放射性。
• 无荧光化合物的干扰

 

V1362   PDE-Glo Phosphodiesterase Assay   品牌 Promega

发表于由

PDE-Glo Phosphodiesterase Assay

货号： V1362      产品名称： PDE-Glo Phosphodiesterase Assay   品牌： Promega 规格： 10,000assays 二周到货 生化试验

说明
   PDE-Glo™ Phosphodiesterase Assay( PDE-Glo™ 磷酸二酯酶检测试剂盒) 是一种发光的、高通量筛选(HTS) 的方法，用来对纯化来源的环状核苷磷酸二酯酶活性进行检测。环状核苷磷酸二酯酶(PDEs) 由于其水解能力，调控了第二信使信号分子cAMP 和cGMP的水平，因此参与了无数的细胞进程。
   PDEs 选择性抑制分子的存在可帮其作为工具用于研究环状核苷信号，并为研究PDEs 在细胞和组织病理方面的作用创造了条件。PDE-Glo™ 磷酸二酯酶检测试剂盒可从化合物文库中筛选和鉴定候选前导化合物。这种检测方法被设计成用于384 孔板，但检测的体系可以很容易的调整为96 或1536 孔平板。PDE-Glo™ 磷酸二酯酶检测试剂盒经过优化可同时检测cAMP和cGMP依赖的磷酸二酯酶。在PDEs 反应完成后，检测所需要的时间从开始到结束不到一小时。
特点  • 多功能
• 可检测cAMP 和cGMP 磷酸二酯酶。
• 灵敏
• 优良的信号: 背景比。 • 可调整至用1536 孔板模式。
• 快速、易用
• 检测可在一小时内完成。
• 均质。
• 行之有效的发光技术
• Ultra-Glo™ 萤光素酶驱动。 • 无放射性。
• 无荧光化合物的干扰

 

BML-SE534-0020   PDE 10A (human) (recombinant) (full-length)   品牌 Biomol

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PDE 10A (human) (recombinant) (full-length)

货号： BML-SE534-0020      产品名称： PDE 10A (human) (recombinant) (full-length)   品牌： Biomol 规格： 20 ug 3周到货 生化实验

PDE10A (full-length) (human), (recombinant)
Product Specification
Alternative Name:Phosphodiesterase 10A
MW:~87kDa
Source:Produced in E. coli. Recombinant human phosphodiesterase 10A (aa 14-779).
UniProt ID:Q9Y233
Formulation:Liquid. In 20mM TRIS-HCl, pH 8.0, containing 150mM sodium chloride and 2mM DTT.
Purity:≥90% (SDS-PAGE)
Application:Study enzyme regulation and kinetics, screen for inhibitors or activators of PDE10A.
Long Term Storage:-80°C
Use/Stability:After rapid initial defrost, aliquot product into individual tubes and refreeze the remaining, unused enzyme quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen, if possible.
Miscellaneous/General:This enzyme can hydrolyze both cAMP and cGMP, but has a higher affinity for cAMP. In the brain it is present in neuronal cell bodies and neuropil of the striatum, in striatonigral and striatopallidal white matter tracks, and in the substantia nigra and globus pallidus. Outside the brain it is found in the testis, epididymal sperm, and enteric ganglia. It is under investigation as a drug target for schizophrenia and diabetes.

-80°C

13602   FAM-cAMP PDE IV substrate *Green fluorescence*   品牌 AAT

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FAM-cAMP PDE IV substrate *Green fluorescence*

货号： 13602      产品名称： FAM-cAMP PDE IV substrate *Green fluorescence*   品牌： AAT 规格： 0.5 umol 二周到货 生化试验

FAM-cAMP PDE IV substrate *Green fluorescence*
Cat # 13602
Storage F/D/L (see storage codes below)
Unit Size 0.5 umol
Ex (nm) 492
Em (nm) 515
MW ~800
Solvent DMSO
This green fluorescent cAMP derivative is a specific substrate for phosphodiesterase (PDE) IV. It can be used for assaying PDE IV activities or screening PDE IV inhibitors in combination with anti-cAMP antibody in a FRET readout or FP format. PDE is a group of enzymes that degrade the second messenger molecules: cyclic nucleotides cAMP and cGMP. They regulate the localization, duration, and amplitude of cyclic nucleotide signaling within subcellular domains. PDEs are therefore important regulators of signal transduction mediated by these second messenger molecules. PDE enzymes are often targets for pharmacological inhibition due to their unique tissue distribution, structural and functional properties. Inhibitors of PDE can prolong or enhance the effects of physiological processes mediated by cAMP or cGMP by inhibition of their degradation by PDE. PDE inhibitors have been identified as new potential therapeutics in areas such as pulmonary arterial hypertension, coronary heart disease, dementia, depression and schizophrenia. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Phosphodiesterases     cAMP GPCR Assays 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)
 

 

 

13604   FAM-cGMP PDE V substrate *Green fluorescence*   品牌 AAT

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FAM-cGMP PDE V substrate *Green fluorescence*

货号： 13604      产品名称： FAM-cGMP PDE V substrate *Green fluorescence*   品牌： AAT 规格： 0.5 umol 二周到货 生化试验

FAM-cGMP PDE V substrate *Green fluorescence*
Cat # 13604
Storage F/D/L (see storage codes below)
Unit Size 0.5 umol
Ex (nm) 492
Em (nm) 515
MW ~800
Solvent DMSO
This green fluorescent cGMP derivative is a specific substrate for phosphodiesterase (PDE) V. It can be used for assaying PDE V activities or screening PDE V inhibitors in combination with anti-cGMP antibody in a FRET readout or FP format. PDE is a group of enzymes that degrade the second messenger molecules: cyclic nucleotides cAMP and cGMP. They regulate the localization, duration, and amplitude of cyclic nucleotide signaling within subcellular domains. PDEs are therefore important regulators of signal transduction mediated by these second messenger molecules. PDE enzymes are often targets for pharmacological inhibition due to their unique tissue distribution, structural and functional properties. Inhibitors of PDE can prolong or enhance the effects of physiological processes mediated by cAMP or cGMP by inhibition of their degradation by PDE. PDE inhibitors have been identified as new potential therapeutics in areas such as pulmonary arterial hypertension, coronary heart disease, dementia, depression and schizophrenia. For example, Sildenafil (Viagra) is an inhibitor of cGMP-specific PDE V, which enhances the vasodilatory effects of cGMP in the corpus cavernosum, and is used to treat erectile dysfunction. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Phosphodiesterases 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

13605   TAMRA-cGMP PDE V substrate *Red fluorescence*   品牌 AAT

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TAMRA-cGMP PDE V substrate *Red fluorescence*

货号： 13605      产品名称： TAMRA-cGMP PDE V substrate *Red fluorescence*   品牌： AAT 规格： 0.5 umol 二周到货 生化试验

-cGMP PDE V substrate *Red fluorescence*

 
Cat # 13605 Storage F/D/L (see storage codes below)
Unit Size 0.5 umol Price $295
Ex (nm) 544 Em (nm) 575
MW ~1000 Solvent DMSO
This red cGMP derivative is a specific substrate for phosphodiesterase (PDE) V. It can be used for assaying PDE V activities or screening PDE V inhibitors in combination with anti-cGMP antibody in a FRET readout or FP format. PDE is a group of enzymes that degrade the second messenger molecules: cyclic nucleotides cAMP and cGMP. They regulate the localization, duration, and amplitude of cyclic nucleotide signaling within subcellular domains. PDEs are therefore important regulators of signal transduction mediated by these second messenger molecules. PDE enzymes are often targets for pharmacological inhibition due to their unique tissue distribution, structural and functional properties. Inhibitors of PDE can prolong or enhance the effects of physiological processes mediated by cAMP or cGMP by inhibition of their degradation by PDE. PDE inhibitors have been identified as new potential therapeutics in areas such as pulmonary arterial hypertension, coronary heart disease, dementia, depression and schizophrenia. For example, Sildenafil (Viagra) is an inhibitor of cGMP-specific PDE V, which enhances the vasodilatory effects of cGMP in the corpus cavernosum, and is used to treat erectile dysfunction.

 

 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Phosphodiesterases 
Storage Codes:

  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)