Cell Meter Caspase 9 Activity Apoptosis Assay Kit * Green Fluorescence*
货号： 22813 产品名称： Cell Meter Caspase 9 Activity Apoptosis Assay Kit * Green Fluorescence* 品牌： AAT 规格： 1 kit 二周到货 生化试验
Cell Meter� Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*
Cat # 22813
Storage F/D/L (see storage codes below)
Unit Size 200 Tests
Ex (nm) 351
Em (nm) 430
Our Cell Meter� assay kits are a set of tools for monitoring cellular functions. There are a variety of parameters that can be used. This particular kit is designed to monitor cell apoptosis by measuring caspase 9 activity. Caspase 9 is a member of the CED-3 subfamily. Activated Caspase-9 cleaves downstream caspases such as caspase-3, -6 and -7, initiating the caspase cascade. It is essential for apoptosis during normal development of the central nervous system. Caspase 9 is proven to have selectivity for the peptide sequence Leu-Glu-His-Asp (LEHD). This kit uses Ac-LEHD-AMC as a fluorogenic indicator for caspase 9 activity. Cleavage of AMC by caspase 9 generates strongly fluorescent AMC which is monitored at Ex/Em = 350/450 nm. The kit provides all the essential components. The assay is robust and can be readily adapted for high throughput screening. It can be used to either quantify the activated caspase 9 activities in apoptotic cells or screen the caspase 9 inhibitors. Quite a few labs have used this kit for high throughput screenings.
Detailed Information: Protocol References MSDS Spectrum
Related Products: Cell Cytotoxicity Apoptosis and Cytotoxicity Cell Apoptosis
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
