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36315   Screen Quest Fluo-8 No Wash Calcium Assay Kit   品牌 AAT

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Screen Quest Fluo-8 No Wash Calcium Assay Kit

货号： 36315      产品名称： Screen Quest Fluo-8 No Wash Calcium Assay Kit   品牌： AAT 规格： 10 Plates 二周到货 生化试验

reen Quest� Fluo-8 No Wash Calcium Assay Kit
Cat # 36315
Storage F/D/L (see storage codes below)
Unit Size 10 Plates
Ex (nm) 490
Em (nm) 525
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest� Fluo-8 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Fluo-8 NW which can cross cell membrane. Fluo-8 NW is the brightest calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Fluo-8 NW are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-8 NW. The characteristics of its long wavelength, high sensitivity, and 100-250 times fluorescence increases (when it forms complexes with calcium) make Fluo-8 NW an ideal indicator for measurement of cellular calcium. This Screen Quest Fluo-8 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

36316   Screen Quest Fluo-8 No Wash Calcium Assay Kit   品牌 AAT

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Screen Quest Fluo-8 No Wash Calcium Assay Kit

货号： 36316      产品名称： Screen Quest Fluo-8 No Wash Calcium Assay Kit   品牌： AAT 规格： 100 Plates 二周到货 生化试验

Screen Quest� Fluo-8 No Wash Calcium Assay Ki
Cat # 36316
Storage F/D/L (see storage codes below)
Unit Size 100 Plates
Ex (nm) 490
Em (nm) 525
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest� Fluo-8 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Fluo-8 NW which can cross cell membrane. Fluo-8 NW is the brightest calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Fluo-8 AM are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Fluo-8 NW. The characteristics of its long wavelength, high sensitivity, and 100-250 times fluorescence increases (when it forms complexes with calcium) make Fluo-8 NW an ideal indicator for measurement of cellular calcium. This Screen Quest Fluo-8 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

 

36320   Screen Quest Fura-2 No Wash Calcium Assay Kit   品牌 AAT

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Screen Quest Fura-2 No Wash Calcium Assay Kit

货号： 36320      产品名称： Screen Quest Fura-2 No Wash Calcium Assay Kit   品牌： AAT 规格： 10 Plates 二周到货 生化试验

Screen Quest� Fura-2 No Wash Calcium Assay Kit
Cat # 36320
Storage F/D/L (see storage codes below)
Unit Size 10 Plates
Ex (nm) 370
Em (nm) 476  
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This ratiometric calcium assay kit allows homogeneous measurement of intracellular calcium changes caused by activation of G-protein-coupled receptors or calcium channels. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fura-2 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Fura-2 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells and its fluorescence wavelength is blue-shifted upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which greatly increase the fluorescence intensity ofFura-2 at the short wavelength. The ratiometric characteristics of Fura-2 make this kit an ideal tool for more accurate measurement of cellular calcium concentration compared to Fluo-4 of the single wavelength. With a single addition, the assay is easy to perform and desirable in a high thoroughput environment. The assay can be used in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation.  
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

36321   Screen Quest Fura-2 No Wash Calcium Assay Kit   品牌 AAT

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Screen Quest Fura-2 No Wash Calcium Assay Kit

货号： 36321      产品名称： Screen Quest Fura-2 No Wash Calcium Assay Kit   品牌： AAT 规格： 100 Plates 二周到货 生化试验

Screen Quest� Fura-2 No Wash Calcium Assay Kit 
Cat # 36321
Storage F/D/L (see storage codes below)
Unit Size 100 Plates
Ex (nm) 370
Em (nm) 476
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This ratiometric calcium assay kit allows homogeneous measurement of intracellular calcium changes caused by activation of G-protein-coupled receptors or calcium channels. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with Fura-2 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Fura-2 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells and its fluorescence wavelength is blue-shifted upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which greatly increase the fluorescence intensity ofFura-2 at the short wavelength. The ratiometric characteristics of Fura-2 make this kit an ideal tool for more accurate measurement of cellular calcium concentration compared to Fluo-4 of the single wavelength. With a single addition, the assay is easy to perform and desirable in a high thoroughput environment. The assay can be used in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation.  
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

36331   Screen Quest Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10 plates*   品牌 AAT

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Screen Quest Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10 plates*

货号： 36331      产品名称： Screen Quest Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10 plates*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Screen Quest� Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10 plates*
Cat # 36331
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 530
Em (nm) 590
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest� Rhod-4 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Rhod-4 NW which can cross cell membrane. Rhod-4 is the brightest red calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Quest Rhod-4� are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Rhod-4. The characteristics of its long wavelength, high sensitivity, and >250 times fluorescence increases (when it forms complexes with calcium) make Quest Rhod-4� an ideal indicator for measurement of cellular calcium. This Screen Quest Rhod-4 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

36332   Screen Quest Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10×10 plates*   品牌 AAT

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Screen Quest Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10×10 plates*

货号： 36332      产品名称： Screen Quest Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10×10 plates*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Screen Quest� Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10×10 plates*
Cat # 36332
Storage F/D/L (see storage codes below)
Unit Size 1 kit 
Ex (nm) 530
Em (nm) 590
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest� Rhod-4 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Rhod-4 NW which can cross cell membrane. Rhod-4 is the brightest red calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Quest Rhod-4� are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Rhod-4. The characteristics of its long wavelength, high sensitivity, and >250 times fluorescence increases (when it forms complexes with calcium) make Quest Rhod-4� an ideal indicator for measurement of cellular calcium. This Screen Quest Rhod-4 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

36334   Screen Quest Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium * *10 plates*   品牌 AAT

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Screen Quest Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium * *10 plates*

货号： 36334      产品名称： Screen Quest Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium * *10 plates*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Screen Quest� Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium * *10 plates*
Cat # 36334
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 530
Em (nm) 590
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest� Rhod-4 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Rhod-4 NW which can cross cell membrane. Rhod-4 is the brightest red calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Quest Rhod-4� are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Rhod-4. The characteristics of its long wavelength, high sensitivity, and >250 times fluorescence increases (when it forms complexes with calcium) make Quest Rhod-4� an ideal indicator for measurement of cellular calcium. This Screen Quest Rhod-4 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

36335   Screen Quest Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium* *10×10 plates*   品牌 AAT

发表于由

Screen Quest Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium* *10×10 plates*

货号： 36335      产品名称： Screen Quest Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium* *10×10 plates*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Screen Quest� Rhod-4 No Wash Calcium Assay Kit *1% FBS Growth Medium* *10×10 plates*
Cat # 36335
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 530
Em (nm) 590  
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). Screen Quest� Rhod-4 NW Calcium Assay Kit provides a homogeneous fluorescence-based assay for detecting the intracellular calcium mobilization. Cells expressing a GPCR of interest that signals through calcium are pre-loaded with our proprietary Rhod-4 NW which can cross cell membrane. Rhod-4 is the brightest red calcium indicator available for HTS screening. Once inside the cell, the lipophilic blocking groups of Quest Rhod-4� are cleaved by non-specific cell esterase, resulting in a negatively charged fluorescent dye that stays inside cells, and its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with screening compounds, the receptor signals release of intracellular calcium, which greatly increase the fluorescence of Rhod-4. The characteristics of its long wavelength, high sensitivity, and >250 times fluorescence increases (when it forms complexes with calcium) make Quest Rhod-4� an ideal indicator for measurement of cellular calcium. This Screen Quest Rhod-4 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

 

36379   Screen Quest FRET No Wash cAMP Assay Kit   品牌 AAT

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Screen Quest FRET No Wash cAMP Assay Kit

货号： 36379      产品名称： Screen Quest FRET No Wash cAMP Assay Kit   品牌： AAT 规格： 1 plate 二周到货 生化试验

Screen Quest� FRET No Wash cAMP Assay Kit
Cat # 36379
Storage F/D/L (see storage codes below)
Unit Size 1 plate
Ex (nm) 390
Em (nm) 650
As our kit 36376, Screen Quest� No Wash FRET cAMP Assay Kit provides another convenient assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. Compared to other commercial ELISA cAMP assay kits, this homogenous cAMP assay kit does not require a wash step or the acetylation step. The assay is based on the competition for a fixed number of anti-cAMP antibody binding sites between the fluorescent cAMP tracer and non-labeled free cAMP. Free cAMP displaces the fluorescent cAMP tracer from the HRP-cAMP/anti-cAMP antibody complex. The anti-cAMP antibody is labeled with our TR Fluor� Tb while the cAMP tracer contains our bFluor� 650. In the absence of cAMP, bFluor� 650-cAMP conjugate is bound to TR Fluor� Tb-labeled anti-cAMP antibody exclusively to have a strong FRET. However, the unlabeled free cAMP in the test sample competes for the TR Fluor� Tb-labeled anti-cAMP antibody conjugate, therefore inhibits the binding of bFluor� 650-cAMP to anti-cAMP antibody. The bFluor� 650 labeled cAMP tracer only has fluorescence lifetime of nanosecond while TR Fluor� Tb-labeled anti-cAMP antibody-bound fluorescent cAMP tracer has much longer fluorescence lifetime value due to the TR-FRET. The magnitude of FRET is proportional to the concentration of cAMP in a sample. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. 
Detailed Information:   Protocol      References      MSDS
Related Products:     cAMP GPCR Assays     Diagnostic Molecules     Cell Signaling 
Storage Codes
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

36380   Screen Quest FRET No Wash cAMP Assay Kit   品牌 AAT

发表于由

Screen Quest FRET No Wash cAMP Assay Kit

货号： 36380      产品名称： Screen Quest FRET No Wash cAMP Assay Kit   品牌： AAT 规格： 10 plates 二周到货 生化试验

Screen Quest� FRET No Wash cAMP Assay Kit
Cat # 36380
Storage F/D/L (see storage codes below)
Unit Size 10 plates
Ex (nm) 390
Em (nm) 650
As our kit 36376, Screen Quest� No Wash FRET cAMP Assay Kit provides another convenient assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. Compared to other commercial ELISA cAMP assay kits, this homogenous cAMP assay kit does not require a wash step or the acetylation step. The assay is based on the competition for a fixed number of anti-cAMP antibody binding sites between the fluorescent cAMP tracer and non-labeled free cAMP. Free cAMP displaces the fluorescent cAMP tracer from the HRP-cAMP/anti-cAMP antibody complex. The anti-cAMP antibody is labeled with our TR Fluor� Tb while the cAMP tracer contains our bFluor� 650. In the absence of cAMP, bFluor� 650-cAMP conjugate is bound to TR Fluor� Tb-labeled anti-cAMP antibody exclusively to have a strong FRET. However, the unlabeled free cAMP in the test sample competes for the TR Fluor� Tb-labeled anti-cAMP antibody conjugate, therefore inhibits the binding of bFluor� 650-cAMP to anti-cAMP antibody. The bFluor� 650 labeled cAMP tracer only has fluorescence lifetime of nanosecond while TR Fluor� Tb-labeled anti-cAMP antibody-bound fluorescent cAMP tracer has much longer fluorescence lifetime value due to the TR-FRET. The magnitude of FRET is proportional to the concentration of cAMP in a sample. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. 
Detailed Information:   Protocol      References      MSDS
Related Products:     cAMP GPCR Assays     Diagnostic Molecules     Cell Signaling 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)