RIPAb+ Fragile X Mental Retardation Protein, clone 1C3

货号： 03-108      产品名称： RIPAb+ Fragile X Mental Retardation Protein, clone 1C3   品牌： Millipore 规格： EA 3周到货 生化实验

RIPAb+ Fragile X Mental Retardation Protein – RIP Validated Antibody and Primer Set
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  RIP, WB, ELISA, ICC, IHC, IH(P), IP Mouse null Monoclonal Antibody
Description:
RIPAb+ Fragile X Mental Retardation Protein
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Trade Name:
Upstate (Millipore)
Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
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Specificity:
FMRP. Cross reaction with FXR protein may be detected in cases of high expression in mice.
Molecular Weight:
71 kDa
Immunogen:
Fusion protein with a full length FMRP (human).
Isotype:
IgG1κ
Background Information:
Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Human, mouse and rat.
Application Notes:
Western Blot Analysis:
Representative lot data.
Mouse E17 spinal cord lysate (Lane 1), Mouse E16 brain (Lane 2) and NIH/3T3 lysate (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Fragile X Mental Retardation Protein (1:500 dilution of a previous lot).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Fragile X Mental Retardation Protein (~71 kDa) (Please see figures).
Immunohistochemistry: Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.
ELISA: A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Detection of FMRP on Blood Smears: A 1:500-1:5,000 dilution of a previous lot was used.
Immunocytochemistry: A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only) (Please see figures).
Optimal working dilutions must be determined by the end user.
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Control:
Includes negative control mouse IgG antibody and control primers specific for mouse Iqsec1.
Quality Assurance:
RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from NIH/3T3 cells (2 x 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal mouse IgG or 5 µL of Anti-Fragile X Mental Retardation Protein antibody and the Magna RI -20°C