A-ADEN REC A2A, 7F6-G5-A2 200UG

货号： 05-717      产品名称： A-ADEN REC A2A, 7F6-G5-A2 200UG   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2
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REFERENCES
Adenosine A2A receptors modulate glutamate uptake in cultured astrocytes and gliosomes.
Matos, Marco, et al. (2012) Glia, 60: 702-16 (2012)
Adenosine A2A receptor in the monkey basal ganglia: Ultrastructural localization and colocalization with the metabotropic glutamate receptor 5 in the striatum.
James W Bogenpohl,Stefanie L Ritter,Randy A Hall,Yoland Smith (2012) The Journal of comparative neurology.520
Detection of antigen interactions ex vivo by proximity ligation assay: endogenous dopamine D2-adenosine A2A receptor complexes in the striatum.
(2011) BioTechniques.51:111-8
Detection of antigen interactions ex vivo by proximity ligation assay: endogenous dopamine D2-adenosine A2A receptor complexes in the striatum.
Pierre Trifilieff,Marie-Laure Rives,Eneko Urizar,Rebecca A Piskorowski,Harshad D Vishwasrao,John Castrillon,Claudia Schmauss,Maria Slättman,Mats Gullberg,Jonathan A Javitch (2011) BioTechniques.51
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Species Reactivity Key Applications Host Format Antibody Type
H, R, M, Rb, Ca  FC, IHC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes adenosine receptor A2A, Mr 43 kDa.
Molecular Weight:
~43 kDa
Immunogen:
6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. Clone 7F6-G5-A2.
Clone:
7F6-G5-A2
Isotype:
IgG2a
Background Information:
Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.
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Species Reactivity:
Human
Rat
Mouse
Rabbit
Canine
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Species Reactivity Note:
Human, mouse, rat, rabbit, and dog. Cross-reactivity with guinea pig also reported.
Application Notes:
Immunohistochemistry: 1 μg/mL of a previous lot detected Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice.
FACS Analysis: A previous lot of this antibody has been reported by an independent laboratory to detect Adenosine Receptor A2A in transfected HEK293 cells (Koshiba, M., 1999).
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Control:
Brain tissue
Quality Assurance:
Routinely evaluated by immunohistochemistry.
Western Blot Analysis:
A 1:1000–1:5000 dilution of this lot of antibody detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras (Rosin, D.L., 1998).
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Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt
UniProt Number:
P29274
Entrez Gene Number:
NM_000675
Gene 2°C – 8°C