pCpGfree-siRNA
货号： kcpgf-sirna 产品名称： pCpGfree-siRNA 品牌： Invivogen 规格： 1 kit 二周到货 生化试验
pCpGfree-siRNA
CpG-free plasmid for siRNA production in vivo
pCpGfree-siRNA is a plasmid that combines the backbone of the pCpGfree plasmids with the siRNA expression cassette of the psiRNA plasmids. It is designed for long lasting expression of shRNAs in vivo as the plasmid does not induce inflammatory responses and gene silencing by methylation in vertebrate hosts.
CpG-free plasmid backbone
Two cloning options: Acc 65I / Hind III or Bbs I / Bbs I
Allows white and blue selection
Selectable in E. coli with Zeocin™
Provided with the psiRNA™ tools:
• LyoComp GT115, lyophilized chemically competent E. coli GT115 cells
• Sequencing primers (OL559-OLSI3′)
• E. coli Fast-Media® Zeo XGal
This product is covered by a Limited Use License (See Terms and Conditions).
kcpg-sirna2 pCpGfree-siRNADUO 品牌 Invivogen
pCpGfree-siRNADUO
货号： kcpg-sirna2 产品名称： pCpGfree-siRNADUO 品牌： Invivogen 规格： 1 kit 二周到货 生化试验
pCpGfree-siRNADUO
pCpGfree-siRNADUO is a newly designed plasmid, completely devoid of CpG dinucleotides. It allows to generate simultaneously two shRNAs for the silencing of either a single target gene (with/without polymorphisms) or two different target genes.
CpG-free plasmid backbone
Two RNA polymerase III cloning cassettes
Allows white and blue selection for each cassette
Selectable in E. coli with Zeocin™
Provided with the psiRNA™ tools:
• LyoComp GT115, lyophilized chemically competent E. coli GT115 cells
• Sequencing primers (OL359-OLSI3′ and OL499-OL1012 pairs)
• E. coli Fast-Media® Zeo XGal and E. coli Fast-Media® Zeo XGluc
This product is covered by a Limited Use License (See Terms and Conditions).
pcpgf-bas pCpGfree-basic 品牌 Invivogen
pCpGfree-basic
货号： pcpgf-bas 产品名称： pCpGfree-basic 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-basic
pCpGfree-basic are reporter plasmids completely devoid of CpG dinucleotides. These plasmids lack the entire promoter region (compared to pCpGfree plasmids).
pCpGfree-basic plasmids contain a multiple cloning site (MCS) upstream of the mSEAP or Lucia, a secreted luciferase, reporter gene. Expression of reporter in cells transfected with this plasmid depends on the insertion of a functional promoter or enhancer/promoter cassette upstream from the reporter gene.
Thus, pCpGfree-basic plasmids allow to study the effect of CpG methylation on a promoter, alone or combined with enhancer elements.
• CpG-free plasmid backbone
• Reporter plasmid without an enhancer/promoter region:
– murine secreted alkaline phosphatase (SEAP)
– secreted luciferase (Lucia)
• Selectable in E. coli with Zeocin™
This product is covered by a Limited Use License (See Terms and Conditions).
pcpgf-lacz pCpGfree-LacZ 品牌 Invivogen
pCpGfree-LacZ
货号： pcpgf-lacz 产品名称： pCpGfree-LacZ 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree
CpGfree is a family of plasmids completely devoid of CpG dinucleotides. Typically, the elements required for replication and selection of the plasmid in E. coli and gene expression in mammalian cells are rich in CpG. In the pCpGfree plasmids these elements are either naturally CpG-free, were modified to remove all CpGs, or entirely synthesized.
• pCpGfree-mcs contains a multiple cloning site (MCS)featuring several commonly used restriction sites, for convenient cloning of a CpG-free gene, such as the genes provided in the pSELECT plasmid or any open reading frame or cDNA.
• pCpGfree-LacZ expresses the lacZΔCpG gene, a CpG-free allele of the LacZ gene.
• pCpGfree-mSEAP expresses the mSEAPΔCpG gene, a CpG-free allele of the mouse secreted embryonic alkaline phosphatase gene.
• pCpGfree-Lucia expresses a CpG-free allele of the novel synthetic secreted luciferase (Lucia) gene.
Selectable in E. coli with Zeocin™
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgf-mcs pCpGfree-mcs 品牌 Invivogen
pCpGfree-mcs
货号： pcpgf-mcs 产品名称： pCpGfree-mcs 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree
CpGfree is a family of plasmids completely devoid of CpG dinucleotides. Typically, the elements required for replication and selection of the plasmid in E. coli and gene expression in mammalian cells are rich in CpG. In the pCpGfree plasmids these elements are either naturally CpG-free, were modified to remove all CpGs, or entirely synthesized.
• pCpGfree-mcs contains a multiple cloning site (MCS)featuring several commonly used restriction sites, for convenient cloning of a CpG-free gene, such as the genes provided in the pSELECT plasmid or any open reading frame or cDNA.
• pCpGfree-LacZ expresses the lacZΔCpG gene, a CpG-free allele of the LacZ gene.
• pCpGfree-mSEAP expresses the mSEAPΔCpG gene, a CpG-free allele of the mouse secreted embryonic alkaline phosphatase gene.
• pCpGfree-Lucia expresses a CpG-free allele of the novel synthetic secreted luciferase (Lucia) gene.
Selectable in E. coli with Zeocin™
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgf-mseap pCpGfree-mSEAP 品牌 Invivogen
pCpGfree-mSEAP
货号： pcpgf-mseap 产品名称： pCpGfree-mSEAP 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree
CpGfree is a family of plasmids completely devoid of CpG dinucleotides. Typically, the elements required for replication and selection of the plasmid in E. coli and gene expression in mammalian cells are rich in CpG. In the pCpGfree plasmids these elements are either naturally CpG-free, were modified to remove all CpGs, or entirely synthesized.
• pCpGfree-mcs contains a multiple cloning site (MCS)featuring several commonly used restriction sites, for convenient cloning of a CpG-free gene, such as the genes provided in the pSELECT plasmid or any open reading frame or cDNA.
• pCpGfree-LacZ expresses the lacZΔCpG gene, a CpG-free allele of the LacZ gene.
• pCpGfree-mSEAP expresses the mSEAPΔCpG gene, a CpG-free allele of the mouse secreted embryonic alkaline phosphatase gene.
• pCpGfree-Lucia expresses a CpG-free allele of the novel synthetic secreted luciferase (Lucia) gene.
Selectable in E. coli with Zeocin™
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgf-prom pCpGfree-promoter 品牌 Invivogen
pCpGfree-promoter
货号： pcpgf-prom 产品名称： pCpGfree-promoter 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-promoter
pCpGfree-promoter is a reporter plasmid completely devoid of CpG dinucleotides.
This plasmid contains a CpG-free allele of a reporter gene (murine SEAP or secreted luciferase Lucia), the human EF-1α promoter and a multiple cloning site in place of the enhancer.
It is specifically designed to analyze the effect of methylation on CpG residues present in enhancer elements.
• CpG-free plasmid backbone
• Reporter plasmid without an enhancer region:
– murine secreted alkaline phosphatase (SEAP)
– secreted luciferase (Lucia)
• Selectable in E. coli with Zeocin™
This product is covered by a Limited Use License (See Terms and Conditions).
pcpgvtb-lz pCpGfree-vitroBLacZ 品牌 Invivogen
pCpGfree-vitroBLacZ
货号： pcpgvtb-lz 产品名称： pCpGfree-vitroBLacZ 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-vitro Blasticidin
Selectable CpG-free Plasmid – Blasticidin Resistance
pCpGfree-vitro Blasticidin is available with a CpG-free allele of the LacZ gene or a multiple cloning site (MCS). This MCS contains several commonly used restriction sites, for convenient cloning of a CpG-free gene or any open reading frame or cDNA.
CpG-free plasmid backbone
Selectable in E. coli and mammalian cells with Blasticidin
pCpGfree-vitroBLacZ: a LacZ expression plasmid
pCpGfree-vitroBmcs: contains a multiple cloning site (MCS)
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgvtb-mcsg2 pCpGfree-vitroBmcs 品牌 Invivogen
pCpGfree-vitroBmcs
货号： pcpgvtb-mcsg2 产品名称： pCpGfree-vitroBmcs 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-vitro Blasticidin
Selectable CpG-free Plasmid – Blasticidin Resistance
pCpGfree-vitro Blasticidin is available with a CpG-free allele of the LacZ gene or a multiple cloning site (MCS). This MCS contains several commonly used restriction sites, for convenient cloning of a CpG-free gene or any open reading frame or cDNA.
CpG-free plasmid backbone
Selectable in E. coli and mammalian cells with Blasticidin
pCpGfree-vitroBLacZ: a LacZ expression plasmid
pCpGfree-vitroBmcs: contains a multiple cloning site (MCS)
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgvth-lz pCpGfree-vitroHLacZ 品牌 Invivogen
pCpGfree-vitroHLacZ
货号： pcpgvth-lz 产品名称： pCpGfree-vitroHLacZ 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-vitro Hygromycin
Selectable CpG-free Plasmids – Hygromycin Resistance
pCpGfree-vitro Hygromycin is available with a CpG-free allele of the LacZ gene or a multiple cloning site (MCS). This MCS contains several commonly used restriction sites, for convenient cloning of a CpG-free gene or any open reading frame or cDNA.
CpG-free plasmid backbone
Selectable in E. coli and mammalian cells with hygromycin
pCpGfree-vitroHLacZ: a LacZ expression plasmid
pCpGfree-vitroHmcs: contains a multiple cloning site (MCS)
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgvth-mcsg2 pCpGfree-vitroHmcs 品牌 Invivogen
pCpGfree-vitroHmcs
货号： pcpgvth-mcsg2 产品名称： pCpGfree-vitroHmcs 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-vitro Hygromycin
Selectable CpG-free Plasmids – Hygromycin Resistance
pCpGfree-vitro Hygromycin is available with a CpG-free allele of the LacZ gene or a multiple cloning site (MCS). This MCS contains several commonly used restriction sites, for convenient cloning of a CpG-free gene or any open reading frame or cDNA.
CpG-free plasmid backbone
Selectable in E. coli and mammalian cells with hygromycin
pCpGfree-vitroHLacZ: a LacZ expression plasmid
pCpGfree-vitroHmcs: contains a multiple cloning site (MCS)
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgvtn-lz pCpGfree-vitroNLacZ 品牌 Invivogen
pCpGfree-vitroNLacZ
货号： pcpgvtn-lz 产品名称： pCpGfree-vitroNLacZ 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-vitro Neomycin
Selectable CpG-free Plasmids – Neomycin Resistance
pCpGfree-vitro Neomycin is available with a CpG-free allele of the LacZ gene or a multiple cloning site (MCS). This MCS contains several commonly used restriction sites, for convenient cloning of a CpG-free gene or any open reading frame or cDNA.
CpG-free plasmid backbone
Selectable with Kanamycin in E. coli and G418 in mammalian cells
pCpGfree-vitroNLacZ: a LacZ expression plasmid
pCpGfree-vitroNmcs: contains a multiple cloning site (MCS)
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgvtn-mcsg2 pCpGfree-vitroNmcs 品牌 Invivogen
pCpGfree-vitroNmcs
货号： pcpgvtn-mcsg2 产品名称： pCpGfree-vitroNmcs 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-vitro Neomycin
Selectable CpG-free Plasmids – Neomycin Resistance
pCpGfree-vitro Neomycin is available with a CpG-free allele of the LacZ gene or a multiple cloning site (MCS). This MCS contains several commonly used restriction sites, for convenient cloning of a CpG-free gene or any open reading frame or cDNA.
CpG-free plasmid backbone
Selectable with Kanamycin in E. coli and G418 in mammalian cells
pCpGfree-vitroNLacZ: a LacZ expression plasmid
pCpGfree-vitroNmcs: contains a multiple cloning site (MCS)
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgf-baslc pCpGfree-basic-Lucia 品牌 Invivogen
pCpGfree-basic-Lucia
货号： pcpgf-baslc 产品名称： pCpGfree-basic-Lucia 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-basic
pCpGfree-basic are reporter plasmids completely devoid of CpG dinucleotides. These plasmids lack the entire promoter region (compared to pCpGfree plasmids).
pCpGfree-basic plasmids contain a multiple cloning site (MCS) upstream of the mSEAP or Lucia, a secreted luciferase, reporter gene. Expression of reporter in cells transfected with this plasmid depends on the insertion of a functional promoter or enhancer/promoter cassette upstream from the reporter gene.
Thus, pCpGfree-basic plasmids allow to study the effect of CpG methylation on a promoter, alone or combined with enhancer elements.
• CpG-free plasmid backbone
• Reporter plasmid without an enhancer/promoter region:
– murine secreted alkaline phosphatase (SEAP)
– secreted luciferase (Lucia)
• Selectable in E. coli with Zeocin™
This product is covered by a Limited Use License (See Terms and Conditions).
tlrl-cpgfn pCpGfree-giant 品牌 Invivogen
pCpGfree-giant
货号： tlrl-cpgfn 产品名称： pCpGfree-giant 品牌： Invivogen 规格： 200 μg 二周到货 生化试验
pCpGfree-giant
CDS Ligand – TLR9 Ligand Control
pCpGfree-giant is a high molecular weight plasmid (~15 kb) entirely devoid of CpG dinucleotides and containing AT-rich regions. This DNA also features no detectable amounts of endotoxin, as determined by the LAL assay and the HEK-Blue™-4 cell line-based assay and no detectable TLR2 activity.
In addition, this plasmid DNA displays no Dcm methylation and a reduced level of Dam methylation. pCpG Giant can be used as a control in studies on CpG methylations.
Moreover, when transfected in ISG-reporter cells, pCpGfree-giant induces strong reporter activity. This activity requires STING as cells deficient for STING fail to respond to transfected pCpGfree-Giant. The CDSs of pCpGfree-giant have not been identified yet, but they probably include IFI16 and DDX41.
pCpGfree-giant is also available complexed with the cationic lipid transfection reagent LyoVec™ to facilitate its uptake.
Catalog code change notification
November, 15th 2012 – The name and catalog code of pCpGfree-giant has been changed from pCpG Giant "tlrl-cpgg" to pCpGfree-giant "tlrl-cpgfn" to notify a change in the product quantity.
Activity:
– CDS agonist
– TLR9 Ligand control
Formulation: naked or complexed with the transfection reagent LyoVec™
Working concentrations:
– 30 ng – 10 μg/ml transfected pCpGfree-giant
– 300 ng – 10 μg/ml pCpGfree-giant/LyoVec™
Endotoxin level: EndoFit™ (<0.001 EU/µg)
Solubility: 1 mg/ml in water
tlrl-cpgfc pCpGfree-giant/LyoVec 品牌 Invivogen
pCpGfree-giant/LyoVec
货号： tlrl-cpgfc 产品名称： pCpGfree-giant/LyoVec 品牌： Invivogen 规格： 100 μg 二周到货 生化试验
pCpGfree-giant
CDS Ligand – TLR9 Ligand Control
pCpGfree-giant is a high molecular weight plasmid (~15 kb) entirely devoid of CpG dinucleotides and containing AT-rich regions. This DNA also features no detectable amounts of endotoxin, as determined by the LAL assay and the HEK-Blue™-4 cell line-based assay and no detectable TLR2 activity.
In addition, this plasmid DNA displays no Dcm methylation and a reduced level of Dam methylation. pCpG Giant can be used as a control in studies on CpG methylations.
Moreover, when transfected in ISG-reporter cells, pCpGfree-giant induces strong reporter activity. This activity requires STING as cells deficient for STING fail to respond to transfected pCpGfree-Giant. The CDSs of pCpGfree-giant have not been identified yet, but they probably include IFI16 and DDX41.
pCpGfree-giant is also available complexed with the cationic lipid transfection reagent LyoVec™ to facilitate its uptake.
Catalog code change notification
November, 15th 2012 – The name and catalog code of pCpGfree-giant has been changed from pCpG Giant "tlrl-cpgg" to pCpGfree-giant "tlrl-cpgfn" to notify a change in the product quantity.
Activity:
– CDS agonist
– TLR9 Ligand control
Formulation: naked or complexed with the transfection reagent LyoVec™
Working concentrations:
– 30 ng – 10 μg/ml transfected pCpGfree-giant
– 300 ng – 10 μg/ml pCpGfree-giant/LyoVec™
Endotoxin level: EndoFit™ (<0.001 EU/µg)
Solubility: 1 mg/ml in water
pcpgf-lucia pCpGfree-Lucia 品牌 Invivogen
pCpGfree-Lucia
货号： pcpgf-lucia 产品名称： pCpGfree-Lucia 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree
CpGfree is a family of plasmids completely devoid of CpG dinucleotides. Typically, the elements required for replication and selection of the plasmid in E. coli and gene expression in mammalian cells are rich in CpG. In the pCpGfree plasmids these elements are either naturally CpG-free, were modified to remove all CpGs, or entirely synthesized.
• pCpGfree-mcs contains a multiple cloning site (MCS)featuring several commonly used restriction sites, for convenient cloning of a CpG-free gene, such as the genes provided in the pSELECT plasmid or any open reading frame or cDNA.
• pCpGfree-LacZ expresses the lacZΔCpG gene, a CpG-free allele of the LacZ gene.
• pCpGfree-mSEAP expresses the mSEAPΔCpG gene, a CpG-free allele of the mouse secreted embryonic alkaline phosphatase gene.
• pCpGfree-Lucia expresses a CpG-free allele of the novel synthetic secreted luciferase (Lucia) gene.
Selectable in E. coli with Zeocin™
These products are covered by a Limited Use License (See Terms and Conditions).
pcpgf-promlc pCpGfree-promoter-Lucia 品牌 Invivogen
pCpGfree-promoter-Lucia
货号： pcpgf-promlc 产品名称： pCpGfree-promoter-Lucia 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-promoter
pCpGfree-promoter is a reporter plasmid completely devoid of CpG dinucleotides.
This plasmid contains a CpG-free allele of a reporter gene (murine SEAP or secreted luciferase Lucia), the human EF-1α promoter and a multiple cloning site in place of the enhancer.
It is specifically designed to analyze the effect of methylation on CpG residues present in enhancer elements.
• CpG-free plasmid backbone
• Reporter plasmid without an enhancer region:
– murine secreted alkaline phosphatase (SEAP)
– secreted luciferase (Lucia)
• Selectable in E. coli with Zeocin™
This product is covered by a Limited Use License (See Terms and Conditions).