pCpGfree-basic-Lucia
货号： pcpgf-baslc 产品名称： pCpGfree-basic-Lucia 品牌： Invivogen 规格： 20 μg 二周到货 生化试验
pCpGfree-basic
pCpGfree-basic are reporter plasmids completely devoid of CpG dinucleotides. These plasmids lack the entire promoter region (compared to pCpGfree plasmids).
pCpGfree-basic plasmids contain a multiple cloning site (MCS) upstream of the mSEAP or Lucia, a secreted luciferase, reporter gene. Expression of reporter in cells transfected with this plasmid depends on the insertion of a functional promoter or enhancer/promoter cassette upstream from the reporter gene.
Thus, pCpGfree-basic plasmids allow to study the effect of CpG methylation on a promoter, alone or combined with enhancer elements.
• CpG-free plasmid backbone
• Reporter plasmid without an enhancer/promoter region:
– murine secreted alkaline phosphatase (SEAP)
– secreted luciferase (Lucia)
• Selectable in E. coli with Zeocin™
This product is covered by a Limited Use License (See Terms and Conditions).
