PKC iota, Active

货号： 7705-5      产品名称： PKC iota, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active PKC iota
Phosphorylate a wide variety of protein targets
Product Overview
Alternate Names / Synonyms: pkc ι, Active PKC ι, PKC ι, PKC i, humanPKC ι, human PKC i, Active kinases, recombinant PKC i, Recombinant PKC ι, recombinant PKC, Kinase activity assay, protein kinase
Gene Symbol: PRKC1
Accession #: NM_002740
Gene ID: 5584
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: ~98.0 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 664 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay in 25µl reaction volume).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: gel pack
Background Information: PKC ι is a member of the protein kinase C family of serine-threonine kinases. The amino acid sequence of PKC ι showed greatest homology to PKC zeta, with 72% identity overall rising to 84% in the catalytic domain. In contrast, the homology of PKC ι to the other isoforms is less pronounced, with < 53% identity even in the highly conserved catalytic region. PKC ι transcript is present predominantly in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. PKC ι is stimulated by tumor necrosis factor α (TNF-α) and is required for the activation of NF-kB by this cytokine. Cell transfections with a PKC ι dominant negative mutant abolished TNF-α-induced NFkB-dependent transcription. PKC ι can modify vulnerability of neural cells to apoptosis induced by amyloid β-peptide (ABP), a cytotoxic peptide linked to neuronal degeneration in Alzheimer’s disease. Associated with the increased resistance to apoptosis are improved mitochondrial function and reduced activity of caspases. In addition, ABP-induced increases in levels of oxidative stress and intracellular calcium levels were attenuated in cells overexpressing PKC ι.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C