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03-249   RIPAb+ Ago1, clone 4G7-E12   品牌 Millipore

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RIPAb+ Ago1, clone 4G7-E12

货号： 03-249      产品名称： RIPAb+ Ago1, clone 4G7-E12   品牌： Millipore 规格： EA 3周到货 生化实验

RIPAb+™ Ago1
Species Reactivity Key Applications Host Format Antibody Type
H  WB, RIP Mouse Purified Monoclonal Antibody
Description:
RIPAb+™ Ago1 Antibody
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Trade Name:
Upstate (Millipore)
Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA-binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Ago1 (Eukaryotic translation initiation factor 2C 1) is a member of the argonaute family. It contains a PAZ and a PIWI domain, and is required for G1 arrest and mating in response to nitrogen starvation. It may also contribute to cell cycle control by regulating cytokinesis. Ago1 is also needed for the production of mature miRNA that affects miRNA-directed RNA cleavage and is therefore important for heterchromatin formation and accurate chromosome segregation.
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Specificity:
This antibody has been shown by an independent laboratory to not cross react with Ago2, Ago3, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
Molecular Weight:
~97 kDa
Immunogen:
GST-tagged recombinant protein corresponding to human Ago1
Isotype:
IgG2aκ
Background Information:
Ago1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react in human
Application Notes:
RNA Binding Protein Immunoprecipitation (microRNA):
Representative lot data.
RIP Lysate prepared from 293 cells (2 X 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG (Cat. # CS200621), or Anti-Ago1 antibody (Cat. # CS207365) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Ago 1-associated microRNA was verified by TaqMan® microRNA assay, hsa-miR-15a. (Figure2). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa cell lysate was probed with Anti-Ago 1, clone 4G7-E12 (0.1 µg/mL). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody -20°C

03-250   RIPAb+ Ago3, clone 4B1-F6   品牌 Millipore

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RIPAb+ Ago3, clone 4B1-F6

货号： 03-250      产品名称： RIPAb+ Ago3, clone 4B1-F6   品牌： Millipore 规格： EA 3周到货 生化实验

RIPAb+™ Ago3
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Species Reactivity Key Applications Host Format Antibody Type
H  ICC, IP, RIP Mouse Purified Monoclonal Antibody
Description:
RIPAb+™ Ago3 Antibody
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Trade Name:
Upstate (Millipore)
Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA-binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Ago3 (Eukaryotic translation initiation factor 2C 3) belongs to the argonaute family and contains a PAZ and a Piwi domain. Ago3 plays a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation.
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Specificity:
This antibody has been shown to not cross react with Ago1, Ago2, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
Molecular Weight:
~96 kDa
Immunogen:
Recombinant protein corresponding to human Ago3
Isotype:
IgG2aκ
Background Information:
Eukaryotic translation initiation factor 2C 3, Ago3, belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain.
Ago3 is indicated to play a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. Unlike Ago1 and Ago2, Ago3 and AG04 do not possess the ability to dissociate miRNA duplexes in a minimal in vitro system. Re-introduction of any Ago variant into embyronic (ES) cells deficient for expression of all four Ago variants will rescue miRNA silencing defects and reduce apoptosis.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react in human
Application Notes:
RNA Binding Protein Immunoprecipitation (microRNA):

Representative lot data.
RIP Lysate prepared from 293 cells (2 X 107 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG (Cat. # CS200621), or Anti-Ago 3 antibody (Cat. # CS207366) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Ago 3-associated microRNA was verified by TaqMan® microRNA assay, hsa-miR-15a. (Figure2). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of HeLa cells using Anti-Ago3, clone 4B1-F6 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.
(Alexa Fluor is a registered trademark of Life Technologies®.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Azuma-Mukai, -20°C

03-251   RIPAb+ IGF2BP2, clone 5E10-3E1   品牌 Millipore

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RIPAb+ IGF2BP2, clone 5E10-3E1

货号： 03-251      产品名称： RIPAb+ IGF2BP2, clone 5E10-3E1   品牌： Millipore 规格： EA 3周到货 生化实验

RIPAb+™ IGF2BP2 Antibody
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, RIP Mouse Purified null
Description:
RIPAb+™ IGF2BP2 Antibody | 03-251
Promotional Text:
Special Offer on Antibodies! Click Here!
Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA-binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) is a member of the IGF-II mRNA-binding protein family that post-transcriptionally regulates IGF2 during developmental stages. IGF2BP1, 2, and 3 bind the UTR region of IGF2 transcripts, and are implicated in the transport of RNA targets, to enable protein synthesis at specific locations in the cytoplasm. Research has shown that the IGF2 gene may be involved in ovarian cancer.
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Molecular Weight:
~66 kDa
Immunogen:
Histidine-tagged recombinant protein corresponding to human IGF2BP2
Isotype:
IgG1κ
Background Information:
Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a member of the IGF-II mRNA binding protein family that posttranscriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family members (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated in the transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. Studies have indicated variations in the IGF2BP2 gene may affect fetal glucose metabolism and plays a role in type 2 diabetes. In addition to playing a role in glucose metabolism and dieabetes, the insulin-like growth factor (IGF) signaling axis plays an important role in cancer biology. Research has shown IGF2 to be emerging as an important gene for ovarian cancer.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react in human
Application Notes:
Western Blot Analysis:
Representative lot data.
HepG2 cell lysate was probed with Anti-IGF2BP2, clone 5E10-3E1 (1:20,000 dilution).
Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates IGF2BP2 (~66 kDa).
Note: An uncharacterized band appears at ~50 kDa in some lysates.
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Control:
Includes normal mouse IgG and primers specific for human IGF2 mRNA.
Quality Assurance:
RNA Binding Protein Immunoprecipitation:
Representative lot data
RIP Lysate prepared from 293 cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 1.25 µg of either a normal mouse IgG or 1.25 µg Anti-IGF2BP2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of IGF2BP2-associated RNA was verified by qPCR using RIP Primers IGF2, (Figure 1).
Please refer to the Magna RIP&tr -20°C

04-1518   Anti-PD-1, clone RMP1-14   品牌 Millipore

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Anti-PD-1, clone RMP1-14

货号： 04-1518      产品名称： Anti-PD-1, clone RMP1-14   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PD-1, clone RMP1-14
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Species Reactivity Key Applications Host Format Antibody Type
M  FC Rat Purified Monoclonal Antibody
Description:
Anti-PD-1 Antibody, clone RMP1-14
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Specificity:
0
Molecular Weight:
32 kDa calculated
Epitope:
Extracellular Domain
Immunogen:
Recombinant protein corresponding to mouse PD-1.
Clone:
RMP1-14
Isotype:
IgG2aκ
Background Information:
PD-1 (Programmed cell death protein 1, CD279) is a single-pass type 1 transmembrane protein that contains an Ig-like V-type domain on the extracellular region and a tyrosine-based motif on the intracellular region. PD-1 functions as an immunoreceptor that is activated by the PD-L1 ligand. PD-1 is expressed on the surface of activated T- and B-cells. It plays an inhibitory role on T-cell function, and may block T-cell—mediated events such as the proliferation of lymphocytes and the secretion of cytokines. PD-1 may therefore negatively regulate immune responses, and disruption of PD-1 function may affect tolerance.
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Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with Mouse.
Control:
BHK cells
Quality Assurance:
Evaluated by Flow Cytometry in BHK cells.

Flow Cytometry Analysis: 0.5 µg of this antibody detected PD-1 in BHK cells.
Purification Method:
Protein G purified
Presentation:
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q02242
Entrez Gene Number:
NP_032824
Gene Symbol:
Pdcd1
Pd1
Alternate Names:
Programmed cell death protein 1
Protein PD-1
mPD-1
CD279
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Flow Cytometry
UniProt Summary:
FUNCTION: Inhibitory cell surface receptor involved in the regulation of T cell function during immunity and tolerance. Upon ligand binding, inhibits T cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors (By similarity).
SUBUNIT STRUCTURE: Monomer.
SUBCELLULAR LOCATION Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Thymus.
DEVELOPMENTAL STAGE: Induced at programmed cell death.
SEQUENCE SIMILARITIES: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
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Product Name:
Anti-PD-1, clone RMP1-14
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Rat
Applications:
Detect PD-1 using this Anti-PD-1 Antibody, clone RMP1-14 validated for use in Flow Cytometry.

2°C – 8°C

04-856-K   ANTI-TBK1, CLONE AOW9 -ML   品牌 Millipore

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ANTI-TBK1, CLONE AOW9 -ML

货号： 04-856-K      产品名称： ANTI-TBK1, CLONE AOW9 -ML   品牌： Millipore 规格： ML 三周到货 生化实验

Anti-TBK1, clone AOW9
Recommended Replacement for: 05-856
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REFERENCES
Triggering the interferon antiviral response through an IKK-related pathway.
Sharma, Sonia, et al. (2003) Science, 300: 1148-51 (2003)
IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway.
Fitzgerald, Katherine A, et al. (2003) Nat. Immunol., 4: 491-6 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H, M, Ca, Ch  WB, IP Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-TBK1 Antibody, clone AOW9, rabbit monoclonal
Replaces:
05-856
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Specificity:
TBK1; Does not cross-react with IKKε, IKKα or IKKβ.
Molecular Weight:
85 kDa
Epitope:
a.a. 642-645
Immunogen:
Peptide corresponding to the sequence C…EEVS… (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.
Clone:
AOW9
Isotype:
IgG
Background Information:
TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.
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Species Reactivity:
Human
Mouse
Canine
Chicken
Species Reactivity Note:
Human. Predicted to cross-react with mouse, dog, and chicken based on sequence homology.
Application Notes:
Immunoprecipitation:
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.
Control:
RIPA lysates from HEK293 cells.
Quality Assurance:
Evaluated by western blot on RIPA lysates from HEK293 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.
Presentation:
Cultured supernatant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q9UHD2
Entrez Gene Number:
NP_037386
Gene Symbol:
EC 2.7.11.1
FLJ11330
NAK
T2K
Alternate Names:
NF-kB-activating kinase
NF-kappa-B-activating kinase
TANK-binding kinase 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Entrez Gene Summary:
The NF-k -20℃

05-503-I   Anti-LacI, clone 9A5   品牌 Millipore

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Anti-LacI, clone 9A5

货号： 05-503-I      产品名称： Anti-LacI, clone 9A5   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-LacI, clone 9A5
Species Reactivity Key Applications Host Format Antibody Type
E. coli  WB Mouse Purified Monoclonal Antibody
Description:
Anti-LacI Antibody, clone 9A5
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Molecular Weight:
~38 kDa observed
Immunogen:
TrpE fused with full-length E. Coli LacI.
Clone:
9A5
Isotype:
IgG1κ
Background Information:
The lactose operon repressor binds to a regulatory element, an operator gene, to form a genetic switch, the lac operon. This switch negatively regulates the expression of structural genes that follow the site of the operator gene. Inducers may bind to the repressor to reduce or eliminate the affinity of the repressor for its operator, and thereby allow for transcription of respective structural genes. Conversely, other effector molecules may increase the binding affinity of the repressor for its operator and thereby inhibit gene transcription. The inducer or effector molecules that associate with a repressor serve as a chemical signal, which monitors the metabolic state of protein(s) expressed by their respective genes.
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Species Reactivity:
E. coli
Species Reactivity Note:
Demonstrated to react with E. Coli.
Control:
Rosetta BL21 (DE3) competent E. Coli cell lysate.
Quality Assurance:
Evaluated by Western Blot in Rosetta™ BL21 (DE3) E. Coli cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected LacI in 10 µg of Rosetta BL21 (DE3) E. Coli cell lysate.
Purification Method:
Protein G purified
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide (This antibody is purified from supernatent.).
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P03023
Entrez Gene Number:
NP_414879
Gene Symbol:
lacI
Alternate Names:
Lactose operon repressor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Product Name:
Anti-LacI, clone 9A5
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 µL
Format:
Purified
Host:
Mouse
Applications:
Anti-LacI Antibody, clone 9A5 is an antibody against LacI for use in Western Blotting.

2°C – 8°C

17-10237   ChIPAb+ HDAC2, clone 3F3   品牌 Millipore

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ChIPAb+ HDAC2, clone 3F3

货号： 17-10237      产品名称： ChIPAb+ HDAC2, clone 3F3   品牌： Millipore 规格： EA 三周到货 生化实验

ChIPAb+™ HDAC2 – ChIP Validated Antibody and Primer Set
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REFERENCES
Recruitment of chromatin-modifying enzymes by CTIP2 promotes HIV-1 transcriptional silencing.
Marban, Céline, et al. (2007) EMBO J., 26: 412-23 (2007)
Species Reactivity Key Applications Host Format Antibody Type
H, B, M, R, Ht  WB, ChIP Mouse Purified Monoclonal Antibody
Description:
ChIPAb+™ HDAC2 – ChIP Validated Antibody and Primer Set
Promotional Text:
Special Shipping Offer on Antibodies
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ HDAC2 set includes the HDAC2 antibody, a Normal Mouse IgG, and control primers which amplify a 92 bp region of ChIP Primers, VWF promoter. The HDAC2 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC2 -associated chromatin.
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Specificity:
This antibody recognizes HDAC2 at the C-terminus.
Molecular Weight:
~60 kDa
Epitope:
C-terminus
Immunogen:
KLH-conjugated, synthetic peptide (CEKTDTKGTKSEQLSNP) corresponding to human HDAC2 at the C-terminus.
Isotype:
IgG1
Background Information:
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.
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Species Reactivity:
Human
Bovine
Mouse
Rat
Hamster
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Species Reactivity Note:
Human, mouse, bovine and hamster. Predicted to cross-react with rat based on sequence homology.
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using using 2 µg of either Normal Mouse IgG or 2 µg of Anti-HDAC2 and the Magna ChIP™ A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of HDAC2 associated DNA fragments was verified by qPCR using ChIP Primers, VWF promoter. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. (Figure 2).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC2 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates HDAC2 (~60 kDa). (Figure 3).
Immunoprecipitation and HDAC Assay:
Representative lot data.
3 μL of a previous lot wa -20℃

CBL412A5   Anti-Tubulin β-III, clone TU-20, Alexa F   品牌 Millipore

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Anti-Tubulin β-III, clone TU-20, Alexa F

货号： CBL412A5      产品名称： Anti-Tubulin β-III, clone TU-20, Alexa F   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Tubulin β-III, clone TU-20, Alexa Fluor®555 Conjugate
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PRODUCT FAMILY INFORMATION
Nervous System Markers
The nervous system coordinates the voluntary and involuntary actions of the individual and transmits signals between different parts of the body.
The nervous system coordinates the voluntary and involuntary actions of the individual and transmits signals between different parts of the body.
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Species Reactivity Key Applications Host Format Antibody Type
R, M, Po, Pm, B  ICC Mouse AlexaFluor®555 Monoclonal Antibody
Description:
Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor®555 Conjugate
Promotional Text:
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Specificity:
This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.
Molecular Weight:
The unconjugated parent antibody (Cat. No. MAB1637) has an observed molecular weight at ~55 kDa.
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to human Tubulin β-III.
Clone:
TU-20
Isotype:
IgG1
Background Information:
Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.
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Species Reactivity:
Rat
Mouse
Pig
Primate
Bovine
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Species Reactivity Note:
Demonstrated to react with Rat. Predicted to react with Mouse, Porcine, Primate, and Bovine based on 100% sequence homology.
Control:
Rat E18 primary cortex cells
Quality Assurance:
Evaluated by Immunocytochemistry in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tubulin β-III in rat E18 primary cortex cells.
Purification Method:
Protein G purified
Presentation:
Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage Conditions:
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Q13509
Entrez Gene Number:
NP_001184110
Gene Symbol:
MC1R
TUBB4
beta-4
Alternate Names:
Tubulin beta-4
Tubulin beta-III
tubulin, beta 3
tubulin, beta, 4
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
Entrez Gene Summary:
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core p 2-8℃