pFUSEss-CHIg-hG1e3

货号： pfusess-hchg1e3      产品名称： pFUSEss-CHIg-hG1e3   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e3
pFUSE-CHIg-hG1e3 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with E233P / L234V / L235A / deltaG236 + A327G / A330S / P331S mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e3 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
DISCLAIMER: pFUSE-CHIg-hG1e3 plasmids featuring the IgG1e3 engineered Fc are sold by InvivoGen under a limited license from Cambridge Enterprise Limited (International Publication Number WO 99/58572).
• pFUSE-CHIg-hG1e3 features the constant region of the human IgG1 heavy chain with E233P / L234V / L235A / ΔG236 + A327G / A330S / P331S mutations
• pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with E233P / L234V / L235A / ΔG236 + A327G / A330S / P331S mutations and the IL2 signal sequence
Characteristics of E233P / L234V / L235A / ΔG236 + A327G / A330S / P331S mutations:
– Reduced binding to FcγRI
– Reduced ADCC and CDC
Both plasmids are selectable with Zeocin™.