pORF-codAupp

货号： porf-codaupp      产品名称： pORF-codAupp   品牌： Invivogen 规格： 1 disk 三周到货 生化试验

codA::upp
Coding sequence in expression plasmid
E. coli Cytosine Deaminase fused to Uracil Phosphoribosyltransferase
Cytosine deaminase (CD) is an enzyme found in prokaryotes and lower eukaryotes but absent in higher eukaryotes. It converts cytosine and its analog, 5-fluorocytosine (5-FC), to uracil and 5-fluorouracil (5-FU) respectively. 5-FU is a toxic compound commonly used as anticancer drug.
To further increase the efficiency of the CD/5-FC pair, InvivoGen has fused the gene encoding uracil phosphoribosyl transferase to the CD gene. UPRT converts 5-FU to 5-FUMP, an irreversible inhibitor of thymidylate synthase.
This gene is available in pUNO1 expression plasmid selectable with Blasticidin.
Bacteria codA::upp (pUNO1-codAupp)
ORF Size: 1932bp
Subclone: NcoI – NheI