EGF, rat recombinant

货号： 4024-20      产品名称： EGF, rat recombinant   品牌： Biovision 规格： 20 μg 3周到货 生化实验

EGF, rat recombinant
A growth factor stimulating cell growth, proliferation, and differentiation by binding to EGFR
Product Overview
Alternate Name/Synonyms: rat EGF, EGF, r-EGF, rr-EGF, recombinant rat EGF, recombinant EGF
Gene Symbol: EGF
Accession #: P07522
Gene ID: Rn. 6075
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from PBS, pH 7.4
Molecular Weight: 6.151 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: N/A
Biological Activity: The ED₅₀ as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 1 x 10⁷units/mg
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile H₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffer
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Epidermal Growth Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6151 Dalton. The Rat EGF is purified by proprietary chromatographic techniques
Amino Acid Sequence: NSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWWKLR
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-20°C