ANTI-PEMT Antibody (C-Terminus)

货号： 07-2051      产品名称： ANTI-PEMT Antibody (C-Terminus)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-PEMT Antibody (CT)
Description:
Anti-PEMT Antibody (CT)
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Specificity:
This antibody recognizes the C-terminal region of human PEMT in Jurkat cell lysates. The antibody easily detected PEMT in Jurkat and L6 lysates by Western blotting, but expression was weak or not detected in A431, HeLa, HEK293, PC12, or NIH/3T3 cell lysates using very dilute antibody (0.25 μg/mL, data not shown). Positive signals in immunocytochemistry using higher concentrations of antibody (2.5 μg/mL) detected PEMT in these cell lines, suggesting differential expression levels of this protein in various cell lines.
Molecular Weight:
~22 kDa
Epitope:
C-terminus
Immunogen:
The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PEMT.
Background Information:
PEMT (phosphatidylethanolamine N-methyltransferase) is an an important enzyme for the synthesis of membrane phospholipids. PEMT converts phosphatidylethanolamine to phosphatidyl-choline by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Inhibition of hepatic PEMT activity has been associated with Alzheimer’s disease and schizophrenia.
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Other homologies: Mouse (86% sequence homology) and Rat (73% sequence homology).
Application Notes:
ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA
Immunocytochemistry:
HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER).
Control:
L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry)
Quality Assurance:
Western Blot Analysis:
1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown).
Purification Method:
Protein A chromatography
Presentation:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Storage Conditions:
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
UniProt Number:
Q9UBM1
Entrez Gene Number:
NP_009100.2
Gene Symbol:
PEAMT
PEMPT
PEMT2
PNMT
Alternate Names:
phosphatidylethanolamine N-methyltransferase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Immunocytochemistry
ELISA
Entrez Gene Summary:
This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms. [provided by Re 2°C – 8°C