pUNO-hTLR4-GFP

货号： phtlr4-gfp      产品名称： pUNO-hTLR4-GFP   品牌： Invivogen 规格： 1 disk 三周到货 生化试验

TLR4::GFP
Plasmid expressing the human TLR4 gene fused to a GFP gene
TLR4-GFP fusion gene was generated by fusing the green fluorescent protein (GFP) gene to the C terminus of human Toll-like receptor 4 (TLR4) gene. This fusion protein can be used to study the localization of TLR4. Transfected cells can be analyzed for GFP expression by flow cytometry and by Western-blotting using GFP antibodies. The TLR4-GFP fusion gene is cloned in the pUNO plasmid under the control of the strong and ubiquitous mammalian promoter EF1α/HTLV.
hTLR4::GFP fusion gene has been fully sequenced, its fluorescence confirmed and its function tested in HEK293 cells coexpressing MD2 and CD14 genes (pDUO-hMD2-CD14) and an NF-κB reporter plasmid (pNiFty-SEAP).
This gene is available in pUNO1 expression plasmid selectable with Blasticidin.
TLR4-GFP (pUNO1-hTLR4-GFP)