PAK4, Active

货号： 7707-5      产品名称： PAK4, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active PAK4
Target for the small GTP binding proteins
Product Overview
Alternate Names / Synonyms: pak-4, pak 4, Active PAK4, PDK4, human PDK4, active kinase, active kinases, PDK, recombinant PDK4, recombinant PDK, kinase activity assay, protein kinase
Gene Symbol: PAK4
Accession #: O96013
Gene ID: 10298
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: ~90.0 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 83 nmol phosphate incorporated into the PKB-sub peptide (CKRPRAASFAE) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: gel pack
Background Information: PAK4 is a recently identified member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, cdc 42 and Rac. PAK4 belongs to the Group II PAKs which also includes PAK5 and PAK6 while Group I PAKs comprise of PAK1, PAK2 and PAK3. PAK4 differs from other members of the PAK family both in sequence and function. PAK4 has been shown to regulate cell morphology and cytoskeletal organization in mammalian cells. PAK4 regulates the activity of LIM kinase 1 which in turn phosphorylates Cofilin leading to cytoskeletal changes. PAK4 can protect cells from apoptosis in response to several different types of stimuli by inhibiting the pro-apoptotic proteins Bad and Caspase 8. PAK4 has been shown to associate with and mediate the downstream signaling of the keratinocyte growth factor receptor. In addition, PAK4 interacts with the β5 integrins and regulates cell migration during engagement of the αvβ5 integrin receptor.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

PAK7, Active

货号： 7727-5      产品名称： PAK7, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active PAK7
Target for the small GTP binding proteins
Product Overview
Alternate Names / Synonyms: pak-7, pak 7, Active PAK7, PAK7, PAK, human PAK, active kinase, active kinases, recombinant PAK7, recombinant PAK, kinase activity assay, protein kinase
Gene Symbol: PAK7
Accession #: Q9P286
Gene ID: 57144
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: 130.0 kDa
Purity by SDS-PAGE: ≥75%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 55 nmol phosphate incorporated into the Akt substrate peptide (CKRPRAASFAE) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: dry ice
Background Information: The p21-activated kinase (PAK) family of protein kinases has recently attracted considerable attention as an effector of Rho family of small G proteins and as an upstream regulator of MAPK signalling pathways during cellular events such as re-arrangement of the cytoskeleton and apoptosis. PAK7 is a novel human PAK family kinase that contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK7 like the other Paks has been implicated in the regulation of cell morphology, motility and transformation.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C