pFUSEss-CHIg-hG1e2

货号： pfusess-hchg1e2      产品名称： pFUSEss-CHIg-hG1e2   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e2
pFUSE-CHIg-hG1e2 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with M252Y / S254T / T256E + H433K / N434F mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e2 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations
• pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations and the IL2 signal sequence
Characteristics M252Y / S254T / T256E + H433K / N434F mutations:
– Increased binding to FcRn
– Increased half-life
Both plasmids are selectable with Zeocin™.