Sonic Hedgehog (SHH), human recombinant

货号： 4010-1000      产品名称： Sonic Hedgehog (SHH), human recombinant   品牌： Biovision 规格： 1 mg 3周到货 生化实验

Sonic Hedgehog (SHH), human recombinant
Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
Product Overview
Alternate Name/Synonyms: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog, recombiant sonic hedgehog ,human hedgehog, human recombinant hedgehog
Gene Symbol: SHH
Accession #: Q15465
Gene ID: 6469
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5
Molecular Weight: 19.697kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: ≥97%
Endotoxin Level: <0.01 ng/μg
Biological Activity: Human recombinant Sonic Hedgehog is biologically active as compared to a standard. The ED50, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 fibroblasts, is 750ng/ml
Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: Recombinant Human Sonic Hedgehog (SHH) is a highly conserved protein that plays an important role in embryonic development. It is expressed in neural tissue, the gut, and areas of limb development and promotes differentiation and growth in a tissue-specific manner. SHH is synthesized as a 45-kDa precursor protein, which is then cleaved to generate the active 19-kDa N-terminus. SHH interacts with the Patched and Smoothened transmembrane receptors, leading to the activation of GLI family transcription factors. Disruption of any part of this pathway during embryogenesis is associated with birth defects ranging from mild to severe. In adults, abnormal activation of the SHH pathway has been implicated in several forms of cancer. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-20°C