标签归档：rad

1703201   IPTG, 1 GRAM   品牌 Bio-Rad

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IPTG, 1 GRAM

货号： 1703201      产品名称： IPTG, 1 GRAM   品牌： Bio-Rad 规格： 1G 蓝白斑筛选

中文品名: 异丙基-β-D-硫代吡喃半乳糖苷 　　商品名称: Isopropyl β-D-1-Thiogalactopyranoside 英文简称: IPTG 　　产品别名: Isopropyl β-D-Thiogalactoside 　　通用CAS : 367-93-1 　　产品纯度: Sigma ≥99%, Merck ≥98% ，INALCO≥99% 　　产品性状: 白色或白色粉末,溶于水后呈清亮无色液体 　　分 子 式: C9H18O5S 　　分 子 量: 238.30 　　保存温度: 2-8°C冷藏保存 ,配制好后保存于-20°C，室温可放置一个月 　　主要作用：异丙基硫代半乳糖苷(IPTG)是一种作用极强的诱导剂,不被细菌代谢而十分稳定,因此被实验室广泛应用 　　IPTG是β–半乳糖苷酶的活性诱导物质。基于这个特性，当pUC系列的载体DNA（或其他带有lacZ 基因载体DNA）以lacZ 缺失细胞为宿主进行转化时、或用M13噬菌体的载体DNA进行转染时，如果在平板培养基中加入X–Gal和IPTG，由于β–半乳糖苷酶的α–互补性，可以根据是否呈现白色菌落（或噬菌斑）而方便地挑选出基因重组体。此外，它还可以作为具有lac或tac等启动子的表达载体的表达诱导物使用

17-10220   LentiBriteTagRFP-Rad51 LentiviralBiosnsr   品牌 Millipore

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LentiBriteTagRFP-Rad51 LentiviralBiosnsr

货号： 17-10220      产品名称： LentiBriteTagRFP-Rad51 LentiviralBiosnsr   品牌： Millipore 规格： EA 三周到货 生化实验

LentiBrite ™ RFP-Rad51 Lentiviral Biosensor
Description:
LentiBrite ™ RFP-Rad51 Lentiviral Biosensor
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 vial (minimum of 3 x 10E8 IFU/mL)
Product Overview:
Read our application note in Nature Methods!
http://www.nature.com/app_notes/nmeth/2012/121007/pdf/an8620.pdf
(Click Here!)
Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click Here)
Biosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time.
Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity.
EMD Millipore is introducing LentiBrite™ Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.
• Pre-packaged, fluorescently-tagged with GFP & RFP
• Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods
• Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells
•Non-disruptive towards cellular function
EMD Millipore’s LentiBrite™ RFP-Rad51 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of Rad51 translocation in difficult-to-transfect cell types.
View All »
Background Information:
Rad51, a eukaryotic RecA-like recombinase, plays a key role in homologous recombination occurring in both the normal cell cycle and repair of DNA damage. However, excess cellular Rad51, as occurs in many cancers, leads to resistance to DNA-damaging radiation and chemotherapy, and genomic instability. Upon DNA damage generating double strand breaks, Rad51 coalesces from the nucleoplasm to discrete nuclear foci, but such foci also occur in the absence of DNA damage when Rad51 is overexpressed. Expression of Rad51 fused to GFP has been employed to trace the dynamics of Rad51 compartmentalization in live cells.
EMD Millipore’s LentiBrite™ RFP-Rad51 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of Rad51 translocation in difficult-to-transfect cell types.
View All »
Key Applications:
Transfection
Immunofluorescence
Immunocytochemistry
Track single cell and cell population migration (migration assay, cell culture)
Application Notes:
Fluorescence Microscopy Imaging:
HeLa cells were plated in a chamber slide and transduced with lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were fixed with formaldehyde and mounted. Images were obtained by oil immersion wide-field fluorescence microscopy. The RFP-Rad51 displays a variably punctate distribution in the nucleus.
Immunocytochemistry Comparison:
U2OS cells were plated in a chamber slide and transduced with lentiviral particles at an MOI of 5 for 24 hours. After 24 hours, -80℃

17-10234   LentiBriteTagGFP2-Rad51 LentiviralBiosen   品牌 Millipore

发表于由

LentiBriteTagGFP2-Rad51 LentiviralBiosen

货号： 17-10234      产品名称： LentiBriteTagGFP2-Rad51 LentiviralBiosen   品牌： Millipore 规格： EA 三周到货 生化实验

LentiBrite ™ GFP-Rad51 Lentiviral Biosensor
Description:
LentiBrite ™ GFP-Rad51 Lentiviral Biosensor
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 vial (minimum of 3 x 10E8 IFU/mL)
Product Overview:
Read our application note in Nature Methods!
http://www.nature.com/app_notes/nmeth/2012/121007/pdf/an8620.pdf
(Click Here!)
Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click Here)
Biosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time.
Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity.
EMD Millipore is introducing LentiBrite™ Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.
• Pre-packaged, fluorescently-tagged with GFP & RFP
• Higher efficiency transfection as compared to traditional chemical-based and other non-viral-based transfection methods
• Ability to transfect dividing, non-dividing, and difficult-to-transfect cell types, such as primary cells or stem cells
•Non-disruptive towards cellular function
EMD Millipore’s LentiBrite™ GFP-Rad51 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of Rad51 translocation in difficult-to-transfect cell types.
View All »
Background Information:
Rad51, a eukaryotic RecA-like recombinase, plays a key role in homologous recombination occurring in both the normal cell cycle and repair of DNA damage. However, excess cellular Rad51, as occurs in many cancers, leads to resistance to DNA-damaging radiation and chemotherapy, and genomic instability. Upon DNA damage generating double strand breaks, Rad51 coalesces from the nucleoplasm to discrete nuclear foci, but such foci also occur in the absence of DNA damage when Rad51 is overexpressed. Expression of Rad51 fused to GFP has been employed to trace the dynamics of Rad51 compartmentalization in live cells.
EMD Millipore’s LentiBrite™ GFP-Rad51 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of Rad51 translocation in difficult-to-transfect cell types.
View All »
Key Applications:
Transfection
Immunofluorescence
Immunocytochemistry
Track single cell and cell population migration (migration assay, cell culture)
Application Notes:
Fluorescence Microscopy Imaging:
HeLa cells were plated in a chamber slide and transduced with lentiviral particles at an MOI of 20 for 24 hours. After media replacement and 48 hours further incubation, cells were fixed with formaldehyde and mounted. Images were obtained by oil immersion wide-field fluorescence microscopy. The GFP-Rad51 displays a variably punctate distribution in the nucleus, and appears to be excluded from nucleoli.
Immunocytochemistry Comparison:
U2OS cells were plated in a chamber slide and transduced with lentiviral particles at -80℃