Sonic Hedgehog (SHH), murine recombinant

货号： 4020-100      产品名称： Sonic Hedgehog (SHH), murine recombinant   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Sonic Hedgehog (SHH), murine recombinant
Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
Product Overview
Alternate Name/Synonyms: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog, recombiant sonic hedgehog ,mouse hedgehog, mouse recombinant hedgehog
Gene Symbol: SHH
Accession #: Q8C765
Gene ID: 20423
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5
Molecular Weight: 19.8 kDa
Purity by SDS-PAGE: ≥97%
Purity by HPLC: ≥97%
Endotoxin Level: <0.01 ng/μg
Biological Activity: Mouse SHH is fully biologically active when compared to standard. Determined by it’s ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED50 in the range of 0.03 – 0.30 ug/ml
Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions
Storage Temp.: -20°C
Shipping: Gel Pack
Background Information: Recombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans.

-20°C