pFUSEss-CHIg-hG1e6

货号： pfusess-hchg1e6      产品名称： pFUSEss-CHIg-hG1e6   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e6
pFUSE-CHIg-hG1e6 is a cloning plasmid that expresses the human IgG1 heavy chain constant region mutated P257I / Q311I . It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e6 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e6 features the constant region of the human IgG1 heavy chain mutated P257I / Q311I
• pFUSEss-CHIg-hG1e6 features the constant region of the human IgG1 heavy chain mutated P257I / Q311I and the IL2 signal sequence
Characteristics of P257I / Q311I mutations:
– Increased binding to FcRn
– Unchanged half-life
Both plasmids are selectable with Zeocin™.