pFUSEss-CHIg-hG1e7

货号： pfusess-hchg1e7      产品名称： pFUSEss-CHIg-hG1e7   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pFUSE-CHIg-hG1e7
pFUSE-CHIg-hG1e7 is a cloning plasmid that expresses the human IgG1 heavy chain constant region mutated K326W / E333S. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hG1e7 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.
• pFUSE-CHIg-hG1e7 features the constant region of the human IgG1 heavy chain mutated K326W / E333S
• pFUSEss-CHIg-hG1e7 features the constant region of the human IgG1 heavy chain mutated K326W / E333S and the IL2 signal sequence
Characteristics of K326W / E333S mutations:
– Increased binding to C1q
– Increased CDC
Both plasmids are selectable with Zeocin™.