Anti-LAMP-1 (CD107a) Species Reactivity Key Applications Host Format Antibody Type H, M, R, Rhesus Macaque WB, ICC Rabbit Affinity Purified Polyclonal Antibody Description: Anti-LAMP-1 (CD107a) Antibody Promotional Text: Special Shipping Offer on Antibodies 100% Performance Guaranteed Specificity: The antibody recognizes LAMP-1 at the topological domain (Lumenal). Molecular Weight: ~ 95kDa observed. Although the calculated MW 45 kDa, LAMP-1 is heavily glycosylated and therefore, MW typically can be expected between 90-120 kDa. Uncharacterized bands may be observed in some lysates. Epitope: Topological domain (Lumenal) Immunogen: KLH-conjugated linear peptide corresponding to human LAMP-1 at the topological domain (Lumenal). Background Information: Lysosome-associated membrane protein 1 (LAMP-1) is a highly glycosolated, single-pass type 1 membrane protein belonging to the lysosome- associated membrane protein family; a group of proteins that make up the majority of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-1 functions to traffic carbohydrate ligands to selectins. It is also thought to play a role in tumor cell metastasis. View All » Species Reactivity: Human Mouse Rat Rhesus Macaque Species Reactivity Note: Demonstrated to react with human, mouse, and rat. Predicted to react with rhesus macaque based on 100% sequence homology. Application Notes: Western Blot (Snap i.d.) Analysis: 1 μg/mL from a previous lot detected LAMP-1 in 10 μg of EL4 cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected LAMP-1 in NIH/3T3, A431, and HeLa cells. Control: EL4 cell lysate Quality Assurance: Evaluated by Western Blot in EL4 cell lysate. Western Blot Analysis: 1 μg/mL of this antibody detected LAMP-1 in 10 μg of EL4 cell lysate. Purification Method: Affinity purified Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. UniProt Number: P11279 Entrez Gene Number: NP_005552 Gene Symbol: LAMP1 LAMP-1 LAMPA LGP120 CD107a View All » Alternate Names: lysosomal-associated membrane protein 1 lysosome-associated membrane glycoprotein 1 Lysosome-associated membrane protein 1 CD107 antigen-like family member A CD107a antigen View All » Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All » Key Applications: Western Blotting Immunocytochemistry Entrez Gene Summary: The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq]. UniProt Summary: FUNCTION: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane. PTM: O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. SEQUENCE SIMILARITIES: Belongs to the LAMP family. View All » Product Name: Anti-LAMP-1 (CD107a) Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. Antibody Type: Polyclonal Antibody Qty/Pk: 100 μg Format: Affinity Purified Host: Rabbit Application 2-8℃
