Ubiquitin, mAb (EX-9)

货号： BML-PW0580-0025      产品名称： Ubiquitin, mAb (EX-9)   品牌： Biomol 规格： 25 ul 3周到货 生化实验

Product Specification
Clone:EX-9
Host:Mouse
Isotype:IgG1
Immunogen:Enzymatically synthesised polyubiquitin chains.
Source:Purified from tissue culture supernatant.
Application:Western Blot: Optimal conditions must be determined individually. A starting dilution of 1:500-1:1’000 is recommended.
Specificity:Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and, to a lesser extent, free ubiquitin.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:-20°C
Long Term Storage:-20°C
Background / Technical Information:Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead.
In order to obtain optimal polyubiquitin lysine linkage independent reactivity immunoblotting membranes must be autoclaved prior to blocking/probing with Ubiquitin, mAb (EX-9). Omitting this step results in decreased reactivity with a slight preference for K63-linked polyubiquitin.

-20°C

Ubiquitin, mAb (EX-9)

货号： BML-PW0580-0100      产品名称： Ubiquitin, mAb (EX-9)   品牌： Biomol 规格： 100 ul 3周到货 生化实验

Product Specification
Clone:EX-9
Host:Mouse
Isotype:IgG1
Immunogen:Enzymatically synthesised polyubiquitin chains.
Source:Purified from tissue culture supernatant.
Application:Western Blot: Optimal conditions must be determined individually. A starting dilution of 1:500-1:1’000 is recommended.
Specificity:Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and, to a lesser extent, free ubiquitin.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:-20°C
Long Term Storage:-20°C
Background / Technical Information:Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead.
In order to obtain optimal polyubiquitin lysine linkage independent reactivity immunoblotting membranes must be autoclaved prior to blocking/probing with Ubiquitin, mAb (EX-9). Omitting this step results in decreased reactivity with a slight preference for K63-linked polyubiquitin.

-20°C

Ubiquitin, mAb (EX-9) (HRP conjugate)

货号： BML-PW0835-0025      产品名称： Ubiquitin, mAb (EX-9) (HRP conjugate)   品牌： Biomol 规格： 25 ul 3周到货 生化实验

Product Specification
Clone:EX-9
Host:Mouse
Isotype:IgG1
Immunogen:Enzymatically synthesised polyubiquitin chains.
Source:Purified from tissue culture supernatant. HRP conjugated chemically in vitro.
Application:Western Blot: Optimal conditions must be determined individually. A starting dilution of 1:500-1:1’000 is recommended.
Specificity:Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and, to a lesser extent, free ubiquitin.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Handling:Avoid freeze/thaw cycles.
Short Term Storage:+4°C
Long Term Storage:+4°C

+4°C

Ubiquitin, mAb (EX-9) (HRP conjugate)

货号： BML-PW0835-0100      产品名称： Ubiquitin, mAb (EX-9) (HRP conjugate)   品牌： Biomol 规格： 100 ul 3周到货 生化实验

Product Specification
Clone:EX-9
Host:Mouse
Isotype:IgG1
Immunogen:Enzymatically synthesised polyubiquitin chains.
Source:Purified from tissue culture supernatant. HRP conjugated chemically in vitro.
Application:Western Blot: Optimal conditions must be determined individually. A starting dilution of 1:500-1:1’000 is recommended.
Specificity:Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and, to a lesser extent, free ubiquitin.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Handling:Avoid freeze/thaw cycles.
Short Term Storage:+4°C
Long Term Storage:+4°C

+4°C