标签归档：cholera

C9903-1MG   Cholera Toxin B subunit|CTB|霍乱毒素B亚基   品牌 Sigma

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Cholera Toxin B subunit|CTB|霍乱毒素B亚基

货号： C9903-1MG      产品名称： Cholera Toxin B subunit|CTB|霍乱毒素B亚基   品牌： Sigma 规格： 1mg

C9903-1MG   Cholera Toxin B subunit|CTB|霍乱毒素B亚基   品牌 Sigma

C9903-1MG   Cholera Toxin B subunit|CTB|霍乱毒素B亚基   品牌 Sigma

Cholera Toxin B subunit 

≥95% (SDS-PAGE), lyophilized powder
assay    ≥95% (SDS-PAGE) 
form    lyophilized powder 
mol wt    mol wt ~12 kDa 
composition    Protein, ~5% Lowry 
total impurities    ≤0.5% Cholera toxin A subunit (SDS-PAGE) 
solubility    H2O: soluble10 mg/mL (stable indefinitely at 2-8 °C.) 
storage temp.    2-8°C 

2-8°C

C8052   Cholera Toxin|Cholergen|Cholera enterotoxin|霍乱毒素|   品牌 Sigma

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Cholera Toxin|Cholergen|Cholera enterotoxin|霍乱毒素|

货号： C8052      产品名称： Cholera Toxin|Cholergen|Cholera enterotoxin|霍乱毒素|   品牌： Sigma 规格： 0.5mg原装 大量现货 科研用

C8052   Cholera Toxin|Cholergen|Cholera enterotoxin|霍乱毒素|   品牌 Sigma

Cholera Toxin from Vibrio cholerae
别名: Cholera enterotoxin, Cholergen
CAS号: 9012-63-9
MDL number: MFCD00130776
包装
Package size based on protein content.
Reconstitution
When reconstituted at 1 mg/mL in water, solution will contain 0.05 M Tris buffer salts, pH 7.5, 0.2 M NaCl, 0.003 M NaN3, ａnd 0.001 M sodium EDTA. Store reconstituted solutions in the refrigerator.
Unit Definition
One unit increases adenylate cyclase activity 6-fold, measured as [3H]-cAMP present, using approx. 70,000 CHO cells after incubation for 90 min at 37 °C with [3H]-adenine followed by a 15 min incubation with 1 mM 3-isobutyl-1-methylxanthine （IBMX）.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide ａnd sodium EDTA
Biochem/physiol Actions
Toxin consisting of an A subunit （27 kDa） surrounded by five B subunits （approximately 12 kDa each）, which attach the toxin to ganglioside GM1 on the cell surface. The A subunit catalyzes ADP-ribosylation of the α-subunit of the stimulatory G protein （Gαs）, reducing GTPase activity ａnd activating the α-subunit. This activation of Gαs leads to an increase in the activity of adenylyl cyclase, resulting in increased levels of cAMP. Also ADP-ribosylates transducin in the eye rod outer segments, inactivating its GTPase activity. Cholera toxin has also been reported to ADP-ribosylate tubulin. Shown to be a potent mucosal vaccine adjuvant, inducing T helper cell type 2 responses by inhibiting the production of interleukin-12.
相关类型 Bacteria, Bioactive Small Molecules, C-CH, Cell Biology, Cell Signaling ａnd Neuroscience,
G Protein Function, G Proteins ａnd Cyclic Nucleotides, Reagents, Toxins ａnd Venoms
Quality Level PREMIUM
assay ~95% （SDS-PAGE）
form lyophilized powder
composition Protein, ~5% Lowry-TCA 
solubility H2O: soluble10 mg/mL
storage temp. 2-8°C

2-8度

BML-G120-0250   Cholera Toxin A Subunit (Vibrio cholerae)   品牌 Biomol

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Cholera Toxin A Subunit (Vibrio cholerae)

货号： BML-G120-0250      产品名称： Cholera Toxin A Subunit (Vibrio cholerae)   品牌： Biomol 规格： 250 ug 3周到货 生化实验

Product Specification
Source:From Vibrio cholerae.
UniProt ID:P01555
Formulation:Lyophilized. Contains sodium azide.
Long Term Storage:+4°C
Miscellaneous/General:The A subunit of cholera toxin possesses ADP-ribosyltransferase activity but is unable to penetrate cells in the absence of the B subunit. It is however, as effective as the native toxin in catalyzing ADP-ribosylation in broken cell preparations.

+4°C

BML-G125-0500   Cholera Toxin B Subunit (Vibrio cholerae)   品牌 Biomol

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Cholera Toxin B Subunit (Vibrio cholerae)

货号： BML-G125-0500      产品名称： Cholera Toxin B Subunit (Vibrio cholerae)   品牌： Biomol 规格： 500 ug 3周到货 生化实验

Product Specification
Source:From Vibrio cholerae.
UniProt ID:P01555
Formulation:Lyophilized. Contains sodium azide.
Long Term Storage:+4°C
Miscellaneous/General:The A subunit of cholera toxin possesses ADP-ribosyltransferase activity but is unable to penetrate cells in the absence of the B subunit. It is however, as effective as the native toxin in catalyzing ADP-ribosylation in broken cell preparations.

+4°C