标签归档：mitochondrial

G8000   Mitochondrial ToxGlo Assay   品牌 Promega

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Mitochondrial ToxGlo Assay

货号： G8000      产品名称： Mitochondrial ToxGlo Assay   品牌： Promega 规格： 10ml 二周到货 生化试验

说明  
 Mitochondrial ToxGloTM Assay ( 线粒体ToxGloTM 检测试剂盒)是一个基于细胞的检测试剂盒，采用按顺序加两次试剂的叠加检测方法来预测外源化合物对线粒体功能异常的可能影响。当细胞短暂暴露于外源化合物时，与空载体处理的对照细胞相比，细胞膜的完整性以及细胞内的ATP 水平可能会有所改变，本检测法基于差别检测与上述两个指标改变相关联的生物标志物。首先，细胞膜的完整性是通过检测与细胞坏死相关联的一个独特的蛋白酶(“死细胞蛋白酶”)的活性有无来实现的，死细胞蛋白酶的活性采用荧光肽底物(bis-AAF-R110) 来测量。bis-AAF-R110 底物不能穿透完整的细胞膜，因此在活细胞中没有信号。其次，ATP 的测定是通过加入ATP 检测试剂来进行的，该试剂能导致细胞裂解，并产生与存在的ATP 量成正比的发光信号。综合这两组数据，可以分析是线粒体功能异常还是非线粒体的细胞毒性相关的机制。
哺乳动物细胞通过线粒体( 氧化磷酸化) 和非线粒体( 糖酵解)的途径产生ATP。为了得到最佳的线粒体反应，需要优化细胞培养条件。用包含半乳糖的培养基来代替添加葡萄糖的培养基可能会增加细胞的氧消耗，并增加线粒体对毒性物质的敏感性
特点 
 •  区别起主导作用的线粒体功能异常与次级的细胞毒性事件： 检测细胞样品的叠加方法，同时检测ATP（最能反应线粒体功能的检测方法）和细胞膜完整性的生物标志物，从而可在同一样品孔中将主要的 线粒体功能障碍与次要的细胞毒性事件区分开来。
•  预测线粒体毒性： 产生与线粒体毒性一致、并与其他引起细胞死亡的非线粒体机制区分开来的数据谱。
•  操作方便： 该试剂盒采用简单的“加入- 混合- 读数”模式。
•  快速： 在1小时内即可快速评估线粒体的潜在功能障碍。
•  性价比高： 采用标准的多模块检测仪器，直接在培养着细胞的平板上进行检测。
•  灵活、易于自动化： 加入试剂的量可以按比例调节，以满足各种通量的需求；该试剂盒可在 96 孔板和 384 孔板上实现自动化。

 

G8001   Mitochondrial ToxGlo Assay   品牌 Promega

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Mitochondrial ToxGlo Assay

货号： G8001      产品名称： Mitochondrial ToxGlo Assay   品牌： Promega 规格： 100ml 二周到货 生化试验

说明  
 Mitochondrial ToxGlo™ Assay （线粒体ToxGlo™ 检测试剂盒）是一个基于细胞的检测试剂盒，采用按顺序加两次试剂的叠加检测方法来预测外源化合物对线粒体功能异常的可能影响。当细胞短暂暴露于外源化合物时，与空载体处理的对照细胞相比，细胞膜的完整性以及细胞内的ATP 水平可能会有所改变，本检测法基于差别检测与上述两个指标改变相关联的生物标志物。首先，细胞膜的完整性是通过检测与细胞坏死相关联的一个独特的蛋白酶（“死细胞蛋白酶”）的活性有无来实现的，死细胞蛋白酶的活性采用荧光肽底物（ bis-AAF-R110 ）来测量。 bis-AAF-R110 底物不能穿透完整的细胞膜，因此在活细胞中没有信号。其次，ATP 的测定是通过加入ATP 检测试剂来进行的，该试剂能导致细胞裂解，并产生与存在的ATP 量成正比的发光信号。综合这两组数据，可以分析是线粒体功能异常还是非线粒体的细胞毒性相关的机制。
哺乳动物细胞通过线粒体（氧化磷酸化）和非线粒体（糖酵解）的途径产生ATP 。为了得到最佳的线粒体反应，优化细胞培养的条件是必要的。用包含半乳糖的培养基来代替添加葡萄糖的培养基可能会增加细胞的氧消耗，并增加线粒体对毒性物质的敏感性。
特点   •  区别起主导作用的线粒体功能异常与次级的细胞毒性事件： 检测细胞样品的叠加方法，同时检测ATP（最能反应线粒体功能的检测方法）和细胞膜完整性的生物标志物，从而可在同一样品孔中将主要的 线粒体功能障碍与次要的细胞毒性事件区分开来。
•  预测线粒体毒性： 产生与线粒体毒性一致、并与其他引起细胞死亡的非线粒体机制区分开来的数据谱。
•  操作方便： 该试剂盒采用简单的“加入- 混合- 读数”模式。
•  快速： 在1小时内即可快速评估线粒体的潜在功能障碍。
•  性价比高： 采用标准的多模块检测仪器，直接在培养着细胞的平板上进行检测。
•  灵活、易于自动化： 加入试剂的量可以按比例调节，以满足各种通量的需求；该试剂盒可在 96 孔板和 384 孔板上实现自动化。

 
 

72620-1KIT   Hu Mitochondrial to Nuclear DNARatioKit   品牌 Merck

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Hu Mitochondrial to Nuclear DNARatioKit

货号： 72620-1KIT      产品名称： Hu Mitochondrial to Nuclear DNARatioKit   品牌： Merck 规格： *** 3周到货 生化实验

 NovaQUANT™ Human Mitochondrial to Nuclear DNA Ratio Kit
产品信息
Features and benefits 
• Optimized qPCR protocol for sensitive, specific and linear detection of mitochondrial or nuclear target genes.
• Eliminate detection of unwarranted mitochondrial pseudogenes in your data.
• Accurately determine DNA ratios based on matched primer pair amplification efficiencies.
• Pre-aliquoted primers for automating your protocols.
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard Handling

-20°C

72621-1KIT   Mo Mitochondrial to Nuclear DNARatioKit   品牌 Merck

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Mo Mitochondrial to Nuclear DNARatioKit

货号： 72621-1KIT      产品名称： Mo Mitochondrial to Nuclear DNARatioKit   品牌： Merck 规格： *** 3周到货 生化实验

NovaQUANT™ Mouse Mitochondrial to Nuclear Ratio kit
Product Description
The NovaQUANT™ Mouse Mitochondrial to Nuclear DNA Ratio Kit compares the levels of nuclear to mitochondrial DNA (mtDNA) in a mouse DNA sample. Real-Time PCR provides a platform for a quantitative assay measuring and comparing mtDNA copy number to that of nuclear DNA (nDNA). This mtDNA/nDNA ratio test kit in qPCR format can be used whenever a small amount of DNA can be extracted from cells or tissues of interest. The amplification primers in this kit have been thoroughly validated for matching amplification efficiency and absence of amplification of mitochondrial pseudogenes.
This kit contains a set of four optimized PCR primer pairs targeting two nuclear and two mitochondrial genes. A single assay is run using four wells, whereby each well individually tests for one of four gene targets (see Figure 1 at the bottom of this page). Resultant Cts obtained from the Real-Time instrument are used to represent the level of each gene. Nuclear gene 1 is compared to that of mitochondrial gene 1 and nuclear gene 2 to mitochondrial gene 2. In this way, ratios are calculated which can be averaged to represent the mtDNA copy number per cell.
Panel Components
• 2 ea           Mouse qPCR plate with pre-aliquoted PCR primer pairs
• 100 ng       Mouse total DNA (10 ng/µl)
• 100 ng       Mouse rho zero DNA (10 ng/µl)
Storage: Store all components at 4 °C for up to three months; we recommend storing the control DNA at –20 °C for long term storage.
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Standard Handling

-20°C

72625-1KIT   NovaQUANT Human Mitochondrial Biogenesis   品牌 Merck

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NovaQUANT Human Mitochondrial Biogenesis

货号： 72625-1KIT      产品名称： NovaQUANT Human Mitochondrial Biogenesis   品牌： Merck 规格： *** 3周到货 生化实验

NovaQUANT™ Human Mitochondrial Biogenesis Kit
Product Description
The NovaQUANT™ Human Mitochondrial Biogenesis qPCR Kit is designed to quantify the relative mRNA levels of complex subunits I, II, III, IV, V, three house-keeping control genes, and a master regulator of oxidative phosphorylation, PGC-1α.
This kit contains HepG2 cDNA as a control and a set of 12 optimized PCR primer pairs, as listed in Technical Bulletin, TB567. The mRNA for the house keeping genes used in this kit are shown to be some of the most stably expressed genes among a different cell types and varying experimental conditions.
Panel Components
• 2 ea            Human Mitochondrial Biogenesis qPCR plate with pre-aliquoted PCR primer pairs
• 100 ng         HepG2 cDNA (10 ng/µl)
Storage: Store all components at 4 °C. This kit is stable for at least three months. For long term storage, store the control cDNA at –20 °C.
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only

-20°C

72626-1KIT   NovaQUANT Mouse Mitochondrial Biogenesis   品牌 Merck

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NovaQUANT Mouse Mitochondrial Biogenesis

货号： 72626-1KIT      产品名称： NovaQUANT Mouse Mitochondrial Biogenesis   品牌： Merck 规格： *** 3周到货 生化实验

NovaQUANT™ Mouse Mitochondrial Biogenesis Kit
Product Description
The NovaQUANT™ Mouse Mitochondrial Biogenesis qPCR Kit is designed to quantify the relative mRNA levels of Oxidative Phosphorylation (OXPHOS) complex subunits I, II, III, IV, V, three house-keeping control genes, and a master regulator of oxidative phosphorylation, PGC-1α.
The kit contains LM cDNA as a control and a set of 12 optimized PCR primer pairs targeting those analytes, which are listed in TB568. The mRNA for the house keeping genes used in this kit are shown to be some of the most stably expressed genes across different cell types and varying experimental conditions.
Panel Components
• 2 ea             Mouse Mitochondrial Biogenesis qPCR plate with pre-aliquoted PCR primer pairs
• 100 ng         LM cDNA (10 ng/µl)
Storage: Store all components at 4 °C. This kit is stable for at least three months. For long term storage, store the control cDNA at –20 °C.
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only

-20°C

AAMT001-1KIT   Mitochondrial Complex I ActivityAssayKit   品牌 Merck

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Mitochondrial Complex I ActivityAssayKit

货号： AAMT001-1KIT      产品名称： Mitochondrial Complex I ActivityAssayKit   品牌： Merck 规格： *** 3周到货 生化实验

Mitochondrial Complex I Activity Assay Kit
Product Description
The Mitochondrial Complex I Activity Assay Kit (Cat. No. AAMT001-1KIT) is used to determine the activity of mitochondrial OXPHOS Complex I (NADH dehydrogenase, E.C. 1.6.5.3). The enzyme complex is immunocaptured within the wells of the microplate and activity is determined by following the oxidation of NADH to NAD+ and the simultaneous reduction of a dye which leads to increased absorbance at 450 nm.
See Figure 1 at the bottom of this page for the Complex I activity reaction scheme. The oxidation of NADH is linked to the reduction of a dye which has increased absorbance at 450 nm and so appears yellow in color.
Panel Components
25 ml  Buffer, (72560-25ML)
10 ml 10X Blocking Solution, (72561-10ML)
1 ml Detergent, (72562-1ML)
1 ea        20X NADH (40 mM), (72563-1EA)
1 ea        100X Dye, (72564-1EA)
1 ea Microplate, (72565-1EA)
* This kit contains sufficient materials for 96 measurements (one 96-well microplate).
Storage: Store all components at 4°C. After hydration of the 20X NADH and 100X dye with dH20, both of these components should be stored be aliquotted and stored at –80°C, as described in the FAQ section of TB554.
This kit contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
The Mitochondrial Complex I Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).
商店和运输信息
Storage  +2°C to +8°C
Ship  Blue Ice Only
Irritant

2°C – 8°C

AAMT002-1KIT   Mitochondrial Complex II Activity Assay   品牌 Merck

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Mitochondrial Complex II Activity Assay

货号： AAMT002-1KIT      产品名称： Mitochondrial Complex II Activity Assay   品牌： Merck 规格： *** 3周到货 生化实验

Mitochondrial Complex II Activity Assay Kit
Product Descriptio
Complex II, also known as succinate-coenzyme Q reductase (SDH, EC 1.3.5.1), is one of the five complexes involved in oxidative phosphorylation in the inner mitochondrial membrane and also a member of the tricarboxylic acid cycle (TCA). It catalyzes electron transfer from succinate to the electron carrier, ubiquinone, but unlike the other four complexes it is not a proton pump. The product ubiquinol is utilized by complex III in the respiratory chain and the product fumarate is necessary to maintain the TCA cycle.
Succinate + ubiquinone (Q) → Fumarate + ubiquinol (QH2)
The Mitochondrial Complex II Activity Assay is designed for determining the Complex II activity in a sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm which in turn recycles the substrate ubiquinone, as shown below.
ubiquinol (QH2) + DCPIP (blue) → ubiquinone (Q) + DCPIPH2 (colorless)
Panel Components
15 ml  20X Buffer, (72581-15ML)
25 ml Complex II Activity Buffer, (72582-25ML)
2 x 1 ml 10X Detergent, (72583-1ML)
500 µl 50X Succinate Solution, (72584-500UL)
250 µl 100X DCPIP, (72585-250UL)
60 µl Ubiquinone 2, (72586-60UL)
5 ml 10X Blocking Solution, (72587-5ML)
6 ml Phospholipids, (72588-6ML)
1 ea Microplate, (72589-1EA)
* This kit contains sufficient materials for 96 measurements (one 96-well microplate).
Storage: Store the 20X Buffer, Complex II Activity Buffer, 10X Detergent, 10X Blocking Solution, and Microplate at 4°C. Store the 50X Succinate Solution, 100X DCPIP,
Ubiquinone 2, and Phospholipids at –80°C The Mitochondrial Complex II Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).
商店和运输信息
Storage  Multiple storage requirements
Ship  Multiple Storage Conditions

–80°C

AAMT006-1KIT   Mitochondrial Cplx IV (Mouse) Act Assay   品牌 Merck

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Mitochondrial Cplx IV (Mouse) Act Assay

货号： AAMT006-1KIT      产品名称： Mitochondrial Cplx IV (Mouse) Act Assay   品牌： Merck 规格： *** 3周到货 生化实验

Mitochondrial Complex IV (Mouse) Activity Assay Kit
Product Description
The Mitochondrial Complex IV (Mouse) Activity Assay Kit is used to determine the activity of the cytochrome c oxidase enzyme (EC 1.9.3.1) in a mouse sample. Complex IV is immunocaptured within the wells and activity is determined colorimetrical by following the oxidation of reduced cytochrome c as an absorbance decrease at 550 nm. The overall reaction is as follows:
4 cytochrome c- + 4 H+ + 4 H+ (in) + O2 → 4 cytochrome c + 2 H2O + 4 H+ (out)
reduced           oxidized
↑Abs 550 nm           ↓Abs 550 nm
Panel Components
10 ml  Buffer, (72616-10ML)
1 ml Detergent, (72617-1ML)
1 ml Reduced Cytochrome C, (72618-1ML)
1 ea Microplate, (72619-1EA)
* This kit contains sufficient materials for 96 measurements (one 96-well microplate).
Storage: Store the Buffer, Detergent, and Microplate at 4°C. Store the Reduced Cytochrome C at –20°C. For multiple experiments, Reduced Cytochrome C may be aliquoted and stored at –20°C or preferably at –80°C.
This kit contains the necessary buffer, detergent for sample preparation, and substrate (reduced cytochrome c) for the reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
The Mitchondrial Complex IV (Mouse) Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).
商店和运输信息
Storage  Multiple storage requirements
Ship  Multiple Storage Conditions

ABC67   Anti-Mitochondrial fission 1 prot (Fis1)   品牌 Millipore

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Anti-Mitochondrial fission 1 prot (Fis1)

货号： ABC67      产品名称： Anti-Mitochondrial fission 1 prot (Fis1)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Mitochondrial fission 1 protein (Fis1)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Mitochondrial fission 1 protein (Fis1) Antibody
Promotional Text:
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Specificity:
This antibody recognizes the cytoplasmic topological domain of Mitochondrial fission 1 protein.
Molecular Weight:
~17 kDa observed
Epitope:
Cytoplasmic topological domain
Immunogen:
KLH-conjugated-linear peptide corresponding to the cytoplasmic topological domain of human Mitochondrial fission 1 protein.
Background Information:
Mitochondrial fission 1 protein, hFIs1, is an overexpressed mammlian protein that is localized to the outer mitochondrial membrane of various cell types. It is involved in the fragmentation of the mitochondrial network and its perinuclear clustering, resulting in the release of cytochrome c from the mitochondrion, and apopotosis. It is known to mediate peroxisomal fission and also plays a critical role in histone function by demethylating Lys-4 of histone H3.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Other homologies: Rat and Mouse (83% sequence homology).
Control:
Raji cell lysate
Quality Assurance:
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Mitochondrial fission 1 protein in 10 µg of Raji cell lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9Y3D6
Entrez Gene Number:
NP_057152
Gene Symbol:
FIS1
TTC11
CGI-135
Alternate Names:
FIS1 homolog
hFis1
Tetratricopeptide repeat protein 11
TPR repeat protein 11
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission.
UniProt Summary:
FUNCTION: Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
SUBUNIT STRUCTURE: Interacts with DNM1L/DLP1 through the TPR region. Interacts with MARCH5.
SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein.
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Product Name:
Anti-Mitochondrial fission 1 protein (Fis1)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect Mitochondrial fission 1 protein (Fis1) using this Anti-Mitochondrial fission 1 protein (Fis1) Antibody validated for use in 2-8℃

10009172-100   JC-1 Mitochondrial Membrane Potential Assay Kit   品牌 Cayman

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JC-1 Mitochondrial Membrane Potential Assay Kit

货号： 10009172-100      产品名称： JC-1 Mitochondrial Membrane Potential Assay Kit   品牌： Cayman 规格： 100 tests 3周

JC-1 Mitochondrial Membrane Potential Assay Kit
Cayman Chemical Item Number 10009172
Description
Mitochondrial membrane potential, Δψm, is an important parameter of mitochondrial function used as an indicator of cell health. JC-1 is a lipophilic, cationic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial Δψm, JC-1 spontaneously forms complexes known as J-aggregates with intense red fluorescence. On the other hand, in apoptotic or unhealthy cells with low Δψm, JC-1 remains in the monomeric form, which shows only green fluorescence. Cayman’s JC-1 Mitochondrial Membrane Potential Assay Kit provides all the necessary reagents, as well as complete instructions, for analysis of mitochondrial integrity in whole cells. The assay is applicable to flow cytometry, fluorescence microscopy, and 96-well plate formats.
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

-20°C