标签归档：mitochondrial

K285-50   Mitochondrial Protein IP Kit   品牌 Biovision

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Mitochondrial Protein IP Kit

货号： K285-50      产品名称： Mitochondrial Protein IP Kit   品牌： Biovision 规格： 50 assays 3周到货 生化实验

Mitochondrial Protein IP Kit
Product Overview
Assay Kit Name:
Mitochondrial Protein IP Kit
Kit Summary:
Application:
• Optimized for compatibility with immunoprecipitation (IP and co-IP) and pull-down using tagged proteins.
• Gentle formulation for maintenance of stable mitochondrial complexes.
• Compatible with SDS PAGE, 2D gel, Blue-Native gel, Mass spectrometry.
• Functional Assays and enzymatic assays.
Sample Type:
• Mitochondria, mitochondrial extract or cell lysate.
Features & Benefits:
• Simple & convenient protocol
• The Kit provides unique formulation of ready-to-use buffers & reagents for IP/Co-IP/Pull down of mitochondrial proteins or complexes.
Kit Components:
• Mitochondria Protein IP Buffer
• Wash Buffer (5X)
• Protease Inhibitor Cocktail
• 10% n-Dodecyl-beta-D-maltoside
• 10% Triton X-100
• 10% Digitonin
Description:
Mitochondria are the power house of the cells and play an essential role in energy production. Damage to the mitochondria activates signaling pathways that induce apoptosis. Mitochondria also regulate and mediate transport of the metabolites and ions needed for oxidative phosphorylation and maintenance of membrane potential for ATP synthesis. Mitochondrial dysfunction leads to several disorders like cardiac dysfunction, diabetes, aging and neurological disorder, mainly caused by mutations in mitochondrial DNA or in nuclear genes that code for mitochondrial components. Thus, mitochondria have several different functions in the cell.
BioVision ready to use mitochondria Protein IP Buffer is optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes. The Mitochondrial Protein IP kit is provided with different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100 and digitonin to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent especially for membrane protein solubilization. However, in case of fragile complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

RDX-RCP9300-C010   Mitochondrial Glycerol-3-Phosphate Dehydrogenase (Western Blot Control) (h) (rec)   品牌 AXXORA

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Mitochondrial Glycerol-3-Phosphate Dehydrogenase (Western Blot Control) (h) (rec)

货号： RDX-RCP9300-C010      产品名称： Mitochondrial Glycerol-3-Phosphate Dehydrogenase (Western Blot Control) (h) (rec)   品牌： AXXORA 规格： 10 ug

22666   Cell Navigator Mitochondrial Staining Kit *Green Fluorescence*   品牌 AAT

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Cell Navigator Mitochondrial Staining Kit *Green Fluorescence*

货号： 22666      产品名称： Cell Navigator Mitochondrial Staining Kit *Green Fluorescence*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Navigator� Mitochondrion Staining Kit *Green Fluorescence*
Cat # 22666
Storage F/D/L (see storage codes below)
Unit Size 500 Assays
Ex (nm) 498
Em (nm) 520 
Our Cell Navigator� fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label mitochondria of live cells in green fluorescence. The kit uses a proprietary dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. 營t is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Labeling Cells     Fuorescence Imaging 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

22667   Cell Navigator Mitochondrial Staining Kit *Orange Fluorescence*   品牌 AAT

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Cell Navigator Mitochondrial Staining Kit *Orange Fluorescence*

货号： 22667      产品名称： Cell Navigator Mitochondrial Staining Kit *Orange Fluorescence*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Navigator� Mitochondrion Staining Kit *Orange Fluorescence*
Cat # 22667
Storage F/D/L (see storage codes below)
Unit Size 500 Assays
Ex (nm) 545
Em (nm) 575
Our Cell Navigator� fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label mitochondria of live cells in orange fluorescence. The cell images can be viewed with the TRITC filter set. The kit uses a proprietary dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. 營t is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Labeling Cells     Fuorescence Imaging 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

22668   Cell Navigator Mitochondrial Staining Kit *Red Fluorescence*   品牌 AAT

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Cell Navigator Mitochondrial Staining Kit *Red Fluorescence*

货号： 22668      产品名称： Cell Navigator Mitochondrial Staining Kit *Red Fluorescence*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Navigator� Mitochondrion Staining Kit *Red Fluorescence*
Cat # 22668
Storage F/D/L (see storage codes below)
Unit Size 500 Assays
Ex (nm) 575
Em (nm) 600
Our Cell Navigator� fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label mitochondria of live cells in red fluorescence. The kit uses a proprietary dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. 營t is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Labeling Cells     Fuorescence Imaging 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

22673   Cell Navigator Mitochondrial Staining Kit *Orange Fluorescence with 405 nm Excitation*   品牌 AAT

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Cell Navigator Mitochondrial Staining Kit *Orange Fluorescence with 405 nm Excitation*

货号： 22673      产品名称： Cell Navigator Mitochondrial Staining Kit *Orange Fluorescence with 405 nm Excitation*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Navigator� Mitochondrion Staining Kit *Orange Fluorescence with 405 nm Excitation*
Cat # 22673
Storage F/D/L (see storage codes below)
Unit Size 500 Assays
Ex (nm) 399
Em (nm) 550
Our Cell Navigator� fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label mitochondria of live cells in orange fluorescence of large Stokes Shift at Ex/Em = 405/550 nm. The kit uses a proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency. The mitochondrial stain used in the kit is quite photostable, making the fluorescence quite robust. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Labeling Cells     Fuorescence Imaging 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

22802   Cell Meter NIR Mitochondrial Membrane Potential Assay Kit *Optimized for Flow Cytometry*   品牌 AAT

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Cell Meter NIR Mitochondrial Membrane Potential Assay Kit *Optimized for Flow Cytometry*

货号： 22802      产品名称： Cell Meter NIR Mitochondrial Membrane Potential Assay Kit *Optimized for Flow Cytometry*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Meter� NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*
Cat # 22802
Storage F/D/L (see storage codes below)
Unit Size 500 Tests  
Ex (nm) 646
Em (nm) 659 
Our Cell Meter� assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. Our Cell Meter� NIR Membrane Potential Detection Kit provides all the essential components with an optimized assay method for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. This fluorometric assay is based on the detection of the mitochondrial membrane potential in cells by our proprietary cationic MitoLite NIR� dye. In normal cells, MitoLite NIR� accumulates primarily in mitochondria, however, in apoptotic cells, MitoLite NIR� staining intensity decreases. Cells stained with MitoLite NIR� can be visualized by flow cytometry with red excitation and far red emission (FL4 channel). The kit can be paired with other reagents, such as blue-excited propidium iodide and Cell Meter� Phosphatidylserine Apoptosis Assay Kit (#22803) for multi-parametric study of cell vitality and apoptosis. The kit is optimized for screening of apoptosis activators and inhibitors by flow cytometry.  
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Cell Cytotoxicity     Apoptosis and Cytotoxicity     Cell Apoptosis 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

22803   Cell Meter NIR Mitochondrial Membrane Potential Assay Kit *Optimized for Microplate Reader*   品牌 AAT

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Cell Meter NIR Mitochondrial Membrane Potential Assay Kit *Optimized for Microplate Reader*

货号： 22803      产品名称： Cell Meter NIR Mitochondrial Membrane Potential Assay Kit *Optimized for Microplate Reader*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Meter� NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Reader*
Cat # 22803
Storage F/D/L (see storage codes below)
Unit Size 500 Tests
Ex (nm) 646
Em (nm) 659 
Our Cell Meter� assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. Our Cell Meter� NIR Mitochondria Membrane Potential Detection Kit provides all the essential components with an optimized assay method for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. This fluorometric assay is based on the detection of the mitochondrial membrane potential in cells by our proprietary cationic MitoLite NIR� dye. In normal cells, MitoLite NIR� accumulates primarily in mitochondria, however, in apoptotic cells, MitoLite NIR� staining intensity decreases. Cells stained with MitoLite NIR� can be monitored fluorimetrically at 660-680 nm with excitation of 620-640 nm. The kit can be used for screening of apoptosis activators and inhibitors. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Cell Cytotoxicity     Apoptosis and Cytotoxicity     Cell Apoptosis 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)
 

22804   Cell Meter Mitochondrial Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*   品牌 AAT

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Cell Meter Mitochondrial Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*

货号： 22804      产品名称： Cell Meter Mitochondrial Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Meter� Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
Cat # 22804
Storage F/D/L (see storage codes below)
Unit Size 500 Tests
Ex (nm) 546
Em (nm) 575 
This particular kit is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential(MMP). The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. Our Cell Meter� Orange Mitochondrial Membrane Potential Assay Kit provides all the essential components with an optimized assay method. This fluorimetric assay uses our proprietary cationic MitoLite� Orange for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. In normal cells, the red fluorescence intensity is increased when MitoLite� Orange is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoLite� Orange is decreased following the collapse of MMP. Cells stained with MitoLite� Orange can be visualized with a flow cytometer at 488 nm excitation with red emission (FL2 channel). The kit can be used together with other reagents, such as Cell Meter� Phosphatidylserine Apoptosis Assay Kit (22835) for multi-parametric study of cell vitality and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Cell Cytotoxicity     Apoptosis and Cytotoxicity     Cell Apoptosis 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)
 

22805   Cell Meter Mitochondrial Membrane Potential Assay Kit *Orange Fluorescence Optimized for Microplate Reader*   品牌 AAT

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Cell Meter Mitochondrial Membrane Potential Assay Kit *Orange Fluorescence Optimized for Microplate Reader*

货号： 22805      产品名称： Cell Meter Mitochondrial Membrane Potential Assay Kit *Orange Fluorescence Optimized for Microplate Reader*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Cell Meter� Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Microplate Reader*
Cat # 22805 Storage F/D/L (see storage codes below)
Unit Size 500 Tests Price $195 
Ex (nm) 546 Em (nm) 575
MW  Solvent 
Our Cell Meter� assay kits are a set of tools for monitoring cellular functions. There are a variety of parameters that can be used. This particular kit is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. This fluorimetric assay uses our proprietary cationic MitoLite� Orange for the detection of the mitochondrial membrane potential change in cells. In normal cells, the orange fluorescence intensity is increased when MitoLite� Orange is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoLite� Orange is decreased following the collapse of MMP. Cells stained with MitoLite� Orange can be fluorometrically monitored at Ex/Em = 540/590 nm. Our Cell Meter� Orange Mitochondrial Membrane Potential Assay Kit provides all the essential components with an optimized assay method. The kit can be used for screening activators and inhibitors of apoptosis. And the assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format without a wash step. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Cell Cytotoxicity     Apoptosis and Cytotoxicity     Cell Apoptosis 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)
 

BML-AK116-0001   Mt-E-Ψ Mitochondrial Permeability Detection Kit   品牌 Biomol

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Mt-E-Ψ Mitochondrial Permeability Detection Kit

货号： BML-AK116-0001      产品名称： Mt-E-Ψ Mitochondrial Permeability Detection Kit   品牌： Biomol 规格： Kit 3周到货 生化试验

The Mît-E-ψ Kit detects the change in mitochondrial permeability which is an early indication of the initiation of cellular apoptosis. The resulting change in membrane potential (δψ), can be detected by a unique fluorescent cationic dye, 5,5’6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzamidazolocarbocyanin iodide (JC-1). Non-apoptotic cells will fluoresce red, and apoptotic cells will fluoresce green. This dye has been incorporated into the user-friendly Mît-E-ψ Kit for the simple and reproducible detection of the collapse of the membrane potential in apoptotic cells. Cells can be analyzed by FACS, fluorescent plate reader, or fluorescence microscopy (excitation between 488-490 nm, and emission at 527 and 590 nm.).
Product Specification
Long Term Storage: +4°C  
Kit/Set Contains: Mît-E-ψ Reagent, Assay Buffer.

+4°C