标签归档：detection

E9032   GloMax-Multi+ Detection System with Instinct Software: Base Instrument with Heating and Shaking   品牌 Promega

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GloMax-Multi+ Detection System with Instinct Software: Base Instrument with Heating and Shaking

货号： E9032      产品名称： GloMax-Multi+ Detection System with Instinct Software: Base Instrument with Heating and Shaking   品牌： Promega 规格： 1 each

G6795   HaloTag Mammalian Protein Detection and Purification System   品牌 Promega

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HaloTag Mammalian Protein Detection and Purification System

货号： G6795      产品名称： HaloTag Mammalian Protein Detection and Purification System   品牌： Promega 规格： 1each 二周到货 生化试验

说明  
 HaloTag ® Mammalian Protein Purification System （HaloTag ® 哺乳动物蛋白纯化系统， Cat.# G6790 ）是专为从哺乳动物细胞培养裂解液中纯化 HaloTag ® 融合蛋白而优化的试剂盒。 HaloTag ® 融合蛋白与 HaloLink™ 树脂形成高度特异的共价键，这种特异性极高的共价结合可以从培养的哺乳动物细胞中纯化并回收到纯度极高的重组蛋白，即使表达水平低时也不受影响。 HaloTag ® Mammalian Protein Detection and Purification System （HaloTag ® 哺乳动物蛋白检测和纯化系统， Cat.# G6795 ）中还包括 HaloTag ® TMRDirect™ Ligand （HaloTag ® TMRDirect™ 配基）。简单易用的 HaloTag ® 融合蛋白的荧光检测方便了表达和纯化条件的快速优化。
特点    •  纯化效率高： HaloLink™ 树脂可以共价结合超过7mg/ml 的 HaloTag ® 融合蛋白，是FLAG ® 结合能力的10倍多。回收效率很高，普遍高于75% 。
•  纯度更高 : 共价捕获使得在广泛的和/或严格的洗脱过程中，不会丢失结合的蛋白，非特异结合率极低（<0.1%），蛋白纯度很高。
•  易于扩展 : 规格可向上或向下扩展，对得到mg级以上的蛋白量非常重要。
•  为哺乳动物蛋白表达而优化： HaloTag ® 平台使得用单一载体在纯化、 pull-down 和细胞成像间的灵活移动成为可能。

 
 

 

G6799   HaloTag Mammalian Protein Detection and Purification System Sample Pack   品牌 Promega

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HaloTag Mammalian Protein Detection and Purification System Sample Pack

货号： G6799      产品名称： HaloTag Mammalian Protein Detection and Purification System Sample Pack   品牌： Promega 规格： 1each 二周到货 生化试验

说明 
 HaloTag ® Mammalian Protein Purification System （HaloTag ® 哺乳动物蛋白纯化系统， Cat.# G6790 ）是专为从哺乳动物细胞培养裂解液中纯化 HaloTag ® 融合蛋白而优化的试剂盒。HaloTag ® 融合蛋白与 HaloLink™ 树脂形成高度特异的共价键，这种特异性极高的共价结合可以从培养的哺乳动物细胞中纯化并回收到纯度极高的重组蛋白，即使表达水平低时也不受影响。 HaloTag ® Mammalian Protein Detection and Purification System （HaloTag ® 哺乳动物蛋白检测和纯化系统， Cat.# G6795 ）中还包括 HaloTag ® TMRDirect™ Ligand （HaloTag ® TMRDirect™ 配基）。简单易用的 HaloTag ® 融合蛋白的荧光检测方便了表达和纯化条件的快速优化。
特点    •  纯化效率高： HaloLink™ 树脂可以共价结合超过7mg/ml 的 HaloTag ® 融合蛋白，是FLAG ® 结合能力的10倍多。回收效率很高，普遍高于75% 。
•  纯度更高 : 共价捕获使得在广泛的和/或严格的洗脱过程中，不会丢失结合的蛋白，非特异结合率极低（<0.1%），蛋白纯度很高。
•  易于扩展 : 规格可向上或向下扩展，对得到mg级以上的蛋白量非常重要。
•  为哺乳动物蛋白表达而优化： HaloTag ® 平台使得用单一载体在纯化、pull-down 和细胞成像间的灵活移动成为可能。

 
 

QIA113-1KIT   CASPASE-8 DETECTION KIT (FITC-IETD-FMK)   品牌 Merck

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CASPASE-8 DETECTION KIT (FITC-IETD-FMK)

货号： QIA113-1KIT      产品名称： CASPASE-8 DETECTION KIT (FITC-IETD-FMK)   品牌： Merck 规格： *** 3周到货 生化实验

Caspase-8 Detection Kit (FITC-IETD-FMK)
A fast, convenient, and sensitive method to measure caspase-8 in living cells. The fluorescent marker, FITC-IETD-FMK, is a labeled, cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspase-8 in living cells. Caspase-8 can be detected by the measurement of fluorescence in a fluorescent microplate reader, flow cytometer, or by fluorescence microscopy.
产品信息
Format  Flow cytometry or fluorescence microscopy
Form  100 Tests
Detection method  Fluorescence
Species reactivity  a broad range of species
Protect from Light  是的
Avoid freeze/thaw  是的
Assay time  1.5 h
Sample type  Intact cells
Kit contains  Labeled Caspase-8 Inhibitor (FITC-IETD-FMK), Wash Buffer, Unlabeled Caspase Inhibitor (Z-VAD-FMK), and a user protocol.
Excitation max.  ~485 nm
Emission max.  ~535 nm
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

QIA115-1KIT   CASPASE-9 DETECTION KIT (FITC-LEHD-FMK)   品牌 Merck

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CASPASE-9 DETECTION KIT (FITC-LEHD-FMK)

货号： QIA115-1KIT      产品名称： CASPASE-9 DETECTION KIT (FITC-LEHD-FMK)   品牌： Merck 规格： *** 3周到货 生化实验

Caspase-9 Detection Kit (FITC-LEHD-FMK)
A fast, convenient, and sensitive method to measure caspase-9 in living cells. The fluorescent marker, FITC-LEHD-FMK, is a labeled, cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspase-9 in living cells. Caspase-9 can be detected by the measurement of fluorescence intensity in a fluorescent microplate reader, flow cytometer, or by fluorescence microscopy.
产品信息
Format  Flow cytometry or fluorescence microscopy
Form  100 Tests
Detection method  Fluorescence
Species reactivity  a broad range of species
Protect from Light  是的
Avoid freeze/thaw  是的
Assay time  1.5 h
Sample type  Intact cells
Kit contains  Labeled Caspase-9 Inhibitor (FITC-LEHD-FMK), Wash Buffer, Unlabeled Caspase Inhibitor (Z-VAD-FMK), and a user protocol.
Excitation max.  ~485 nm
Emission max.  ~535 nm
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

QIA117-1KIT   SENESCENCE DETECTION KIT   品牌 Merck

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SENESCENCE DETECTION KIT

货号： QIA117-1KIT      产品名称： SENESCENCE DETECTION KIT   品牌： Merck 规格： *** 3周到货 生化实验

Senescence Detection Kit
Useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. Higher levels of β-Galactosidase is considered as a good marker for senescent cells. The mechanism of this is believed to be due to the increased lysosomal content of senescent cells, resulting in an increase of the lysosomal enzyme β-galactosidase. Cells can be observed using microscope.
产品信息
Format  12-well plate
Form  250 Tests
Detection method  Colorimetric
Protect from Light  是的
Assay time  30 min. plus overnight incubation
Sample type  Intact cells
Kit contains  Fixative Solution, X-Gal, Staining Solution, Staining Supplement, and a user protocol.
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

QIA20-1EA   CELL DEATH DETECTION (NMP) ELISA KIT   品牌 Merck

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CELL DEATH DETECTION (NMP) ELISA KIT

货号： QIA20-1EA      产品名称： CELL DEATH DETECTION (NMP) ELISA KIT   品牌： Merck 规格： *** 3周到货 生化实验

Cell Death Detection (Nuclear Matrix Protein) ELISA Kit
Designed for the quantitative detection of NMP41/7. The specific detection of soluble human NMPs afforded by the Cell Death Detection (Nuclear Matrix Protein) ELISA allows quantitation of cell death.
产品信息
Format  96-well plate
Form  96 Tests
Detection method  Colorimetric
Species reactivity  human
Applications 
Enzyme-Linked Immunosorbent Assay
Do not freeze  是的
Unit definition  One unit (U) is defined as the amount of NMP 41/7 released by 850 MCF7 cells maintained at a density of 1 – 5 x 105 cells/ml in serum-free medium for 7 days.
Sensitivity  10 U/ml
Assay range  15-1000 U/ml
Assay time  4 h
Sample type  Tissue culture supernatant
Kit contains  Coated 96-Well Removable Strip Plate, Lyophilized NMP Standard, NMP Detector Antibody, Anti-FITC-POD, Substrate, ELISA Conjugant Diluent, 20X Plate Wash Concentrate, Stop Solution, Plate Sealer, and a user protocol.
Declaration  Use of this product is covered by U.S. Patents 5,783,403, 5,882,876, 5,989,826, 6,162,607, 6,410,247, and 6,740,494. Foreign patents also cover the use of this product in numerous jurisdictions.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage  -20°C
Ship  Blue Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

QIA90-1KIT   CASPASE DETECTION KIT(FITC-VAD-FMK)   品牌 Merck

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CASPASE DETECTION KIT(FITC-VAD-FMK)

货号： QIA90-1KIT      产品名称： CASPASE DETECTION KIT(FITC-VAD-FMK)   品牌： Merck 规格： *** 3周到货 生化实验

Caspase Detection Kit (FITC-VAD-FMK)
A convenient and sensitive method for the detection of activated caspases in living cells. The fluorescent marker, FITC-VAD-FMK, is a cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspases in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence plate reader, or flow cytometry.
产品信息
Format  Flow Cytometry or Fluorimeter
Form  100 Tests
Detection method  Fluorescence
Species reactivity  a broad range of species
Avoid freeze/thaw  是的
Assay time  1.5 h
Sample type  Intact cells
Kit contains  Labeled Caspase Inhibitor (FITC-VAD-FMK), Wash Buffer, Caspase Inhibitor (Z-VAD-FMK), and a user protocol.
Excitation max.  ~485 nm
Emission max.  ~535 nm
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

QIA91-1KIT   CASPASE-3 DETECTION KIT (FITC-DEVD-FMK)   品牌 Merck

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CASPASE-3 DETECTION KIT (FITC-DEVD-FMK)

货号： QIA91-1KIT      产品名称： CASPASE-3 DETECTION KIT (FITC-DEVD-FMK)   品牌： Merck 规格： *** 3周到货 生化实验

Caspase-3 Detection Kit (FITC-DEVD-FMK)
A convenient and sensitive method for the detection of activated caspase-3 in living cells. The fluorescent marker, FITC-DEVD-FMK, is a cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspase-3 in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence plate reader, or flow cytometry.
产品信息
Format  Flow cytometry, fluorescence microscopy
Form  100 Tests
Detection method  Fluorescence
Species reactivity  a broad range of species
Avoid freeze/thaw  是的
Assay time  1.5 h
Sample type  Cultured cells
Kit contains  Labeled Caspase-3 Inhibitor (FITC-DEVD-FMK), Wash Buffer, Caspase-3 Inhibitor (Z-VAD-FMK), and a user protocol.
Excitation max.  ~485 nm
Emission max.  ~535 nm
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

QIA92-1KIT   CASPASE DETECTION KIT (RED-VAD-FMK)   品牌 Merck

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CASPASE DETECTION KIT (RED-VAD-FMK)

货号： QIA92-1KIT      产品名称： CASPASE DETECTION KIT (RED-VAD-FMK)   品牌： Merck 规格： *** 3周到货 生化实验

Caspase Detection Kit (Red-VAD-FMK)
A convenient and sensitive method for the detection of activated caspases in living cells. The fluorescent marker is VAD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspases in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader or flow cytometry.
产品信息
Format  Flow cytometry or fluorescence microscopy
Form  100 Tests
Detection method  Fluorescence
Species reactivity  a broad range of species
Avoid freeze/thaw  是的
Assay time  1.5 h
Sample type  Intact cells
Kit contains  Labeled Caspase Inhibitor (Red-VAD-FMK), Wash Buffer, Caspase Inhibitor (Z-VAD-FMK), and a user protocol
Excitation max.  ~540 nm
Emission max.  ~570 nm
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

QIA93-1KIT   CASPASE-3 DETECTION KIT (RED-DEVD-FMK)   品牌 Merck

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CASPASE-3 DETECTION KIT (RED-DEVD-FMK)

货号： QIA93-1KIT      产品名称： CASPASE-3 DETECTION KIT (RED-DEVD-FMK)   品牌： Merck 规格： *** 3周到货 生化实验

Caspase-3 Detection Kit (Red-DEVD-FMK)
A convenient and sensitive method for the detection of activated caspase-3 in living cells. The fluorescent marker is DEVD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspase-3 in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader, or flow cytometry.
产品信息
Format  Flow cytometry or fluorescence microscopy
Form  100 Tests
Detection method  Fluorescence
Species reactivity  a broad range of species
Avoid freeze/thaw  是的
Assay time  1.5 h
Sample type  Cultured cells
Kit contains  Labeled Caspase-3 Inhibitor (Red-DEVD-FMK), Wash Buffer, Caspase-3 Inhibitor (Z-VAD-FMK), and a user protocol.
Excitation max.  ~540 nm
Emission max.  ~570 nm
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C

V8920   Luciferin Detection Reagent   品牌 Promega

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Luciferin Detection Reagent

货号： V8920      产品名称： Luciferin Detection Reagent   品牌： Promega 规格： 10ml 二周到货 生化试验

说明
   萤光素前体底物可被用于检测细胞色素P450 或NAT2 的特定异构体的活性，这些底物的名称反映了异构体的种类。Luciferin-MultiCYP 是一个混合底物，可与至少21 种P450 酶的异构体发生反应；在检测多种P450 酶混合物的净活性时非常适用。Luciferin-NAT2 是N- 乙酰基转移酶2 (NAT2) 的绝好底物，NAT2 为II 期生物转化酶，可将外源化合物中的芳香基团进行乙酰化。该底物几乎未显示出与NAT1 的交叉反应。

 
 
 

V8921   Luciferin Detection Reagent   品牌 Promega

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Luciferin Detection Reagent

货号： V8921      产品名称： Luciferin Detection Reagent   品牌： Promega 规格： 50ml 二周到货 生化试验

说明
   萤光素前体底物可被用于检测细胞色素P450 或NAT2 的特定异构体的活性，这些底物的名称反映了异构体的种类。Luciferin-MultiCYP 是一个混合底物，可与至少21 种P450 酶的异构体发生反应；在检测多种P450 酶混合物的净活性时非常适用。Luciferin-NAT2 是N- 乙酰基转移酶2 (NAT2) 的绝好底物，NAT2 为II 期生物转化酶，可将外源化合物中的芳香基团进行乙酰化。该底物几乎未显示出与NAT1 的交叉反应。

 
 

V8930   Luciferin Detection Reagent with esterase   品牌 Promega

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Luciferin Detection Reagent with esterase

货号： V8930      产品名称： Luciferin Detection Reagent with esterase   品牌： Promega 规格： 10ml 二周到货 生化试验

说明
    萤光素前体底物可被用于检测细胞色素P450 或NAT2 的特定异构体的活性，这些底物的名称反映了异构体的种类。Luciferin-MultiCYP 是一个混合底物，可与至少21 种P450 酶的异构体发生反应；在检测多种P450 酶混合物的净活性时非常适用。Luciferin-NAT2 是N- 乙酰基转移酶2 (NAT2) 的绝好底物，NAT2 为II 期生物转化酶，可将外源化合物中的芳香基团进行乙酰化。该底物几乎未显示出与NAT1 的交叉反应。