标签归档：pan

ST1098-50UL   ANTI-PAN TORC (1-42) RABBIT PAB   品牌 Merck

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ANTI-PAN TORC (1-42) RABBIT PAB

货号： ST1098-50UL      产品名称： ANTI-PAN TORC (1-42) RABBIT PAB   品牌： Merck 规格： *** 3周到货 生化实验

Anti-PAN TORC (1-42) Rabbit pAb
Anti-MECT1, Anti-TORC1, Anti-TORC2, Anti-TOR
C3, Anti-Transducer of CREB protein 1, Anti-Transducer of regulated cAMP response element-binding protein (CREB), Anti-Mucoepidermoid carcinoma
translocated protein 1
Recognizes the ~79 kDa TORC1, TORC2, and TORC3 proteins in 293T cells over-expressing TORC1, TORC2, or TORC3. Recognizes endogenous TORC1 and TORC2.
产品信息
Host  Rabbit
Isotype  IgG
Immunogen  a recombinant protein consisting of amino acids 1-42 of human TORC1 fused to GST
Form  Liquid
Formulation  Undiluted serum.
Species reactivity  human, mouse, rat
Preservative  None
Applications 
Immunoblotting (Western Blotting)
Immunoprecipitation
Positive control  293T cells over-expressing TORC1, TORC2, or TORC3, HepG2, NIH3T3, Min6, and FAO cells
商店和运输信息
Storage  +2°C to +8°C
Ship  Blue Ice Only
Standard Handling

2°C – 8°C

03-248   RIPAb+ pan Ago, clone 2A8   品牌 Millipore

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RIPAb+ pan Ago, clone 2A8

货号： 03-248      产品名称： RIPAb+ pan Ago, clone 2A8   品牌： Millipore 规格： EA 3周到货 生化实验

RIPAb+™ pan Ago – RIP Validated Antibody and Primer Set
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REFERENCES
A high throughput experimental approach to identify miRNA targets in human cells.
Tan, Lu Ping, et al. (2009) Nucleic Acids Res., 37: e137 (2009)
Involvement of AGO1 and AGO2 in mammalian transcriptional silencing.
Janowski, Bethany A, et al. (2006) Nat. Struct. Mol. Biol., 13: 787-92 (2006)
Comparative expression analysis of the MAGED genes during embryogenesis and brain development.
Mathieu Bertrand,Ivo Huijbers,Patrick Chomez,Olivier De Backer (2004) Developmental dynamics : an official publication of the American Association of Anatomists.230
Species Reactivity Key Applications Host Format Antibody Type
H  WB, IP, ICC, IHC, RIP Mouse Purified Monoclonal Antibody
Description:
RIPAb+™ pan Ago – RIP Validated Antibody and Primer Set
Promotional Text:
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100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Argonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways.
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Specificity:
This antibody recognizes Ago1, Ago2, Ago3, and Ago4. Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)
Molecular Weight:
~ 95 and 70 kDa observed. This antibody recognizes Ago1, Ago2, Ago3, and Ago4.
Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)
Immunogen:
Histidine – tagged recombinant protein corresponding to human Ago.
Isotype:
IgG1κ
Background Information:
Argonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Western Blot Analysis:
Representative lot data.
HeLa cell lysate was probed with Anti-pan Ago, clone 2A8 (0.5 μg/mL). Proteins were visual -20°C

ABN161   Anti-pan Neurexin-1-alpha   品牌 Millipore

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Anti-pan Neurexin-1-alpha

货号： ABN161      产品名称： Anti-pan Neurexin-1-alpha   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-pan Neurexin-1
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REFERENCES
The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Bottos, Alessia, et al. (2009) Proc. Natl. Acad. Sci. U.S.A., 106: 20782-7 (2009)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IHC, ICC, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-pan Neurexin-1 Antibody
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Specificity:
Pan Neurexin 1 and there is a 75% overlap with Neurexin 2 and 3.
Molecular Weight:
~160 kDa observed
Epitope:
C terminus, 100% conserved between alpha and beta versions of Neurexin 1.
Immunogen:
KLH-conjugated linear peptide corresponding to human pan Neurexin-1-alpha.
Background Information:
Neurexin (NRXN) is a presynaptic single pass transmembrane protein that assists in joining neurons together at the synapse. Neurexins mediate signaling across the synapse and neural networks by specifying synpatic function. Studies have shown mutations in the genese encoding for neurexins are impicated in autism, schizphrenia, and nicotine dependence. There are two classifications of neurexins, α-NRXNs and β-NRXNs. The α-NRXNs are larger and have different amino-terminal extracellular sequences than β-NRXNs. β-NRXNs acta as receptors for neuroligin and has been found to play a role in angiogenesis. Pan-neurexin antibodies recognize all neurexin isoforms.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Human, Mouse, and Rat.
Application Notes:
Immunohistochemistry Analysis: A 1:500-1:1,000 dilution from a representative lot detected pan Neurexin-1-alpha in normal mouse cerebellum and normal rat cerebellum tissues.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in E18 chicken embryo arteries. (Bottos, A., et al. (2009). Proc Natl Acad Sci USA. 106(49):20782-20787.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in E18 chicken brain arteries. (Bottos, A., et al. (2009). Proc Natl Acad Sci USA. 106(49):20782-20787.)
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Control:
Mouse brain membrane tissue lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain membrane tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected pan Neurexin-1-alpha on 10 µg of mouse brain membrane tissue lysate.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in preservative free buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Q9ULB1
Entrez Gene Number:
NP_001129131
Gene Symbol:
NRXN1
Alternate Names:
Neurexin-1-alpha
Neurexin I-alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key App -20℃

ABN2300   Neuro-Chrom Pan Neuronal Marker- Rabbit   品牌 Millipore

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Neuro-Chrom Pan Neuronal Marker- Rabbit

货号： ABN2300      产品名称： Neuro-Chrom Pan Neuronal Marker- Rabbit   品牌： Millipore 规格： EA 三周到货 生化实验

Neuro-Chrom™ Pan Neuronal Marker-Rabbit
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Species Reactivity Key Applications Host Format Antibody Type
R, M  ICC, IHC, IF Rabbit null Polyclonal Antibody
Description:
Neuro-Chrom™ Pan Neuronal Marker-Rabbit
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Special Offer on Antibodies! Click Here!
Trade Name:
Neuro-Chrom
Specificity:
Cat. # ABN2300 is specific to axons (neurites), dendrites, nucleus, and the cell body of neurons.
Epitope:
Whole Neuron Marker
Background Information:
Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques such as Golgi staining and modern molecular approaches such as GFP constructs yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, Millipore has developed a polyclonal antibody blend that reacts against key somatic, nuclear, dendritic, and axonal proteins distributed across the pan-neuronal architecture that can then be detected by a single secondary antibody. This antibody cocktail has been validated in diverse methods, cell culture and immuno-histochemistry, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology.
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Species Reactivity:
Rat
Mouse
Species Reactivity Note:
Rat and mouse. Reactivity with other species has not been determined.
Control:
Rat E18 cortex primary neuron cell culture or mouse adult brain cryosections.
Quality Assurance:
Routinely tested Rat E18 cortex primary neurons in Immunocytochemistry.
Presentation:
Blended polyclonal antibody cocktail in PBS with 0.05% NaN3
Storage Conditions:
Maintain at 2-8°C for up to 1 year from date of receipt.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunocytochemistry
Immunohistochemistry
Immunofluorescence
Product Name:
Neuro-Chrom™ Pan Neuronal Marker-Rabbit
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Host:
Rabbit

2-8℃