RIPAb+ pan Ago, clone 2A8

货号： 03-248      产品名称： RIPAb+ pan Ago, clone 2A8   品牌： Millipore 规格： EA 3周到货 生化实验

RIPAb+™ pan Ago – RIP Validated Antibody and Primer Set
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REFERENCES
A high throughput experimental approach to identify miRNA targets in human cells.
Tan, Lu Ping, et al. (2009) Nucleic Acids Res., 37: e137 (2009)
Involvement of AGO1 and AGO2 in mammalian transcriptional silencing.
Janowski, Bethany A, et al. (2006) Nat. Struct. Mol. Biol., 13: 787-92 (2006)
Comparative expression analysis of the MAGED genes during embryogenesis and brain development.
Mathieu Bertrand,Ivo Huijbers,Patrick Chomez,Olivier De Backer (2004) Developmental dynamics : an official publication of the American Association of Anatomists.230
Species Reactivity Key Applications Host Format Antibody Type
H  WB, IP, ICC, IHC, RIP Mouse Purified Monoclonal Antibody
Description:
RIPAb+™ pan Ago – RIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Argonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways.
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Specificity:
This antibody recognizes Ago1, Ago2, Ago3, and Ago4. Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)
Molecular Weight:
~ 95 and 70 kDa observed. This antibody recognizes Ago1, Ago2, Ago3, and Ago4.
Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)
Immunogen:
Histidine – tagged recombinant protein corresponding to human Ago.
Isotype:
IgG1κ
Background Information:
Argonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Application Notes:
Western Blot Analysis:
Representative lot data.
HeLa cell lysate was probed with Anti-pan Ago, clone 2A8 (0.5 μg/mL). Proteins were visual -20°C