A-ACET-HIST H4(K5), CHIP GRD 100UL

货号： 07-327      产品名称： A-ACET-HIST H4(K5), CHIP GRD 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H4 (Lys5)
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REFERENCES
HDA19 is required for the repression of salicylic acid biosynthesis and salicylic acid-mediated defense responses in Arabidopsis.
Sun-Mee Choi,Hae-Ryong Song,Soon-Ki Han,Muho Han,Chi-Yeol Kim,Jaejin Park,Yong-Hwan Lee,Jong-Seong Jeon,Yoo-Sun Noh,Bosl Noh (2012) The Plant journal : for cell and molecular biology.71
HBO1 is required for H3K14 acetylation and normal transcriptional activity during embryonic development.
Andrew J Kueh,Mathew P Dixon,Anne K Voss,Tim Thomas (2011) Molecular and cellular biology.31
Histone crosstalk between H3S10ph and H4K16ac generates a histone code that mediates transcription elongation.
Zippo, Alessio, et al. (2009) Cell, 138: 1122-36 (2009)
Activation of bovine somatic cell nuclear transfer embryos by PLCZ cRNA injection.
Pablo J Ross, Ramon M Rodriguez, Amy E Iager, Zeki Beyhan, Kai Wang, Neli P Ragina, Sook-Young Yoon, Rafael A Fissore, Jose B Cibelli, Pablo J Ross, Ramon M Rodriguez, Amy E Iager, Zeki Beyhan, Kai Wang, Neli P Ragina, Sook-Young Yoon, Rafael A Fissore, Jose B Cibelli (2009) Reproduction (Cambridge, England).137:427-37
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, DB, ICC, ChIP-seq, ChIP Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H4 (Lys5) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes the N-terminus of Histone H4 acetylated at Lys5.
Molecular Weight:
~13 kDa observed
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminus of Histone H4 acetylated at Lys5.
Modifications:
Acetylation
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human based on 100% sequence homology. This antibody is expected to demonstrate broad species cross-reactivity.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected acetyl-Histone H4 in HeLa and A431 cells.
Dot Blot Specificity Analysis: A representative lot detected acetyl-histone H4 (Lys5) peptides, but not unacetylated-histone H4 (Lys4) peptides or other peptides corresponding to modified histones.
Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated acetyl-Histone H4 in HeLa S3 chromatin.
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Control:
HeLa acid extract
Quality Assurance:
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H4 in 10 µg of HeLa acid extract.
Purification Method:
Purified by immunodepletion
Presentation:
Immunodepleted rabbit serum with 0.05% soduim azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C -20℃

A-ACET-HIST H4(K8), CHIP GRD 100UL

货号： 07-328      产品名称： A-ACET-HIST H4(K8), CHIP GRD 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H4 (Lys8) Antibody
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REFERENCES
Role of histone acetylation in the stimulatory effect of valproic acid on vascular endothelial tissue-type plasminogen activator expression.
Pia Larsson,Erik Ulfhammer,Mia Magnusson,Niklas Bergh,Sebastian Lunke,Assam El-Osta,Robert L Medcalf,Per-Arne Svensson,Lena Karlsson,Sverker Jern (2012) PloS one.7
Analysis of model replication origins in Drosophila reveals new aspects of the chromatin landscape and its relationship to origin activity and the pre-RC.
Liu J, McConnell K, Dixon M, Calvi BR (2011) Molecular biology of the cell
Hasegawa Y, Takahashi N, Forrest AR, Shin JW, Kinoshita Y, Suzuki H, Hayashizaki Y (2011) Stem cells (Dayton, Ohio)
Domains of Tra1 important for activator recruitment and transcription coactivator functions of SAGA and NuA4 complexes.
Knutson BA, Hahn S (2011) Mol Cell Biol.31:818-31. Epub 2010 Dec 13.
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Species Reactivity Key Applications Host Format Antibody Type
H, Yeast (S. cerevisiae)  IP, WB, DB, ChIP-seq Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H4 (Lys8) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes acetyl-Histone H4 (Lys8).
Molecular Weight:
10 kDa
Immunogen:
Peptide corresponding to amino acids 3-13 of yeast Histone H4 (RGKGGAcKGLGKG-C) in which lysine 8 is acetylated.
Modifications:
Acetylation
Isotype:
IgG
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
The N-terminal tail of histone H4 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Species Reactivity:
Human
Yeast (S. cerevisiae)
Control:
Sodium butyrate treated HeLa cells
Quality Assurance:
routinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells
Purification Method:
Unpurified
Presentation:
Unpurified rabbit polyclonal in serum with 0.05% sodium azide.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P62805
Entrez Gene Number:
NM_175054
Gene Symbol:
HIST1H4A
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIST1H4B
H4FE
H4/M
H4/a
HIST1H4E
HIST4H4
HIST2H4A
H4FC
H4FI
H4/H
HIST1H4C
H4/G
HIST1H4K
H4FJ
H4FD
H4/I
H4/B
H4/D
H4/C
HIST1H4J
HIST1H4D
H4/E
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Alternate Names:
H4K8Ac
Histone H4 (acetyl K8)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unaut -20℃

A-ACET-HIST H4(K16) 100UL

货号： 07-329      产品名称： A-ACET-HIST H4(K16) 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H4 (Lys16) Antibody
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REFERENCES
Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination.
Tang, Jiangbo, et al. (2013) Nat. Struct. Mol. Biol., 20: 317-25 (2013)
BCL6 controls neurogenesis through Sirt1-dependent epigenetic repression of selective Notch targets.
Tiberi, Luca, et al. (2012) Nat. Neurosci., (2012)
Sir3 and Epigenetic Inheritance of Silent Chromatin in Saccharomyces cerevisiae.
Tina Motwani,Minakshi Poddar,Scott G Holmes (2012) Molecular and cellular biology.32
FoxA1 and glucocorticoid receptor crosstalk via histone H4K16 acetylation at a hormone regulated enhancer.
Belikov S, Holmqvist PH, Astrand C, Wrange O (2012) Experimental cell research.318:61-74. Epub 2011 Oct 6.
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Yeast (S. cerevisiae)  WB, Mplex, PIA, DB, ChIP-seq, ChIP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-acetyl-Histone H4 (Lys16) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Histone H4 acetylated on lysine 16, but not histone H4 containing a lysine 16 to arginine substitution.
Molecular Weight:
10 kDa
Epitope:
Lys16
Immunogen:
Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.
Modifications:
Acetylation
Isotype:
IgG
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
View All »
Species Reactivity:
Human
Vertebrates
Yeast (S. cerevisiae)
Species Reactivity Note:
Yeast and human. Broad species cross-reactivity is expected.
Application Notes:
Multiplexing:
This antibody specifically recognizes histone H4 acetylated on Lys16 by Luminex® assay.
Peptide Inhibition Assay:
This antibody peptide blocked on HeLa cell extracts.
Chromatin Immunoprecipitation
An independent laboratory has shown that this antibody preferentially immunopre-cipitates chromatin from wild type yeast, compared to yeast containing a histone H4 lysine 16 to arginine substitution.
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Control:
TSA treated NIH/3T3 cells.
HeLa acid extract
Quality Assurance:
Evaluated by western blot on acid extracts of HeLa cells.
Western Blot Analysis:
0.1 µg/mL of this antibody detected Histone H4 acetylated on Lys16 in 10 µg of HeLa Acid extract cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8° C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
P62805
Entrez Gene Number:
NM_175054
Gene Symbol:
HIST1H4A
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIST1H4B
H4FE
H4/M
H4/a
HI 2-8℃

A-ACET-HIST H3 (K18) CHIP GRD 100

货号： 07-354      产品名称： A-ACET-HIST H3 (K18) CHIP GRD 100   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H3 (Lys18) Antibody
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REFERENCES
cAMP response element modulator (CREM)α suppresses IL-17F expression in T lymphocytes from patients with systemic lupus erythematosus (SLE).
Hedrich, Christian M, et al. (2011) The Journal of biological chemistry, (2011)
cAMP-responsive element modulator (CREM)α protein induces interleukin 17A expression and mediates epigenetic alterations at the interleukin-17A gene locus in patients with systemic lupus erythematosus.
Rauen, Thomas, et al. (2011) J. Biol. Chem., 286: 43437-46 (2011)
cAMP-responsive element modulator (CREM)α protein signaling mediates epigenetic remodeling of the human interleukin-2 gene: implications in systemic lupus erythematosus.
Hedrich, Christian M, et al. (2011) J. Biol. Chem., 286: 43429-36 (2011)
Regulation of TCR Vγ2 gene rearrangement by the helix-loop-helix protein, E2A.
Masatoshi Nozaki,Kousho Wakae,Nobuyuki Tamaki,Shuji Sakamoto,Kiyouhisa Ohnishi,Toshifumi Uejima,Nagahiro Minato,Itaru Yanagihara,Yasutoshi Agata (2011) International immunology.23
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Yeast (S. cerevisiae)  WB, DB, ChIP, ChIP-seq Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H3 (Lys18) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
For the full resolution version of the ChIP-seq data Click Here.
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Histone H3 acetylated on lysine 18.
Molecular Weight:
17 kDa
Immunogen:
KLH-conjugated, synthetic peptide (GKAPRAcKQLASK-C) corresponding to amino acids 13-23 of yeast Histone H3 acetylated on lysine 18with a C-terminal cysteine added for conjugation purposes
Modifications:
Acetylation
Background Information:
Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
View All »
Species Reactivity:
Human
Vertebrates
Yeast (S. cerevisiae)
Species Reactivity Note:
Yeast, Human, Most common vertebrates
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Normal Rabbit Serum , or 2 µL of Anti-Acetyl-Histone H3 (Lys18)and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Acetyl-Histone H3 (Lys18) associated DNA fragments was verified by qPCR using Control Primers specific for the human GAPDH promoter region as a positive locus, and β-globin primers as a negative locus. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
ChIP-Sequencing:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 2 μg of anti-acetyl-Histone H3 (Lys18) antibody (cat# 07-354), 20 µL Protein A/G beads , and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols w -20℃

A-ACET-HIST H3(K23), CHIP GRD 100UL

货号： 07-355      产品名称： A-ACET-HIST H3(K23), CHIP GRD 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H3 (Lys23)
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REFERENCES
Corepressor-directed preacetylation of histone H3 in promoter chromatin primes rapid transcriptional switching of cell-type-specific genes in yeast.
Alec M Desimone,Jeffrey D Laney (2010) Molecular and cellular biology.30
Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin.
Suka, N, et al. (2001) Mol. Cell, 8: 473-9 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Yeast (S. cerevisiae)  WB, DB, ChIP Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H3 (Lys23) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Histone H3 acetylated on lysine 23. Does not recognize unacetylated recombinant histone H3
Molecular Weight:
17kDa
Immunogen:
Ovalbumin-conjugated, synthetic peptide (KQLASAcKAARK-C) corresponding to amino acids 18-27 of yeast histone H3 acetylated on lysine 23 with a C-terminal cysteine added for conjugation purposes
Modifications:
Acetylation
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
View All »
Species Reactivity:
Human
Vertebrates
Yeast (S. cerevisiae)
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit serum or 2 µL Anti-acetyl-Histone H3 (Lys23)and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of acetyl-Histone H3 (Lys23) associated DNA fragments were verified by qPCR using Control Primers specific for the human GAPDH promoter region as a positive locus, and MyoD primers as a negative locus. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Acid extracts from sodium butyrate treated HeLa cells (Lane 1, Catalog # 17-305) and recombinant Histone H3 (Lane 2, Catalog # 14-494) were probed with Anti-acetyl-Histone H3 (Lys23) (1:100,000 dilution).
Arrow indicates acetyl histone H3 (~17 kDa)
Dot Blot:
Representative lot data.
40 ng and 4ng amounts of histone peptides with various modifications (see table 1) were transferred to PVDF membrane and probed with Anti-Acetyl-Histone H3 (Lys23) antibody (1:2000 dilution). Proteins were visualized using a goat anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Image from a 60 second exposure is shown.
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Control:
Acid extracts from sodium butyrate treated HeLa cells
Quality Assurance:
routinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells
Purification Method:
Antiserum
Presentation:
Rabbit antiserum containing 0.05% -20℃

A-ACET-HIST H3(K27) 100UL

货号： 07-360      产品名称： A-ACET-HIST H3(K27) 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H3 (Lys27) Antibody
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REFERENCES
Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila.
Kathryn Parker,Julia Maxson,Alissa Mooney,Emily A Wiley (2007) Eukaryotic cell.6
Arabidopsis GCN5, HD1, and TAF1/HAF2 interact to regulate histone acetylation required for light-responsive gene expression.
Benhamed, Moussa, et al. (2006) Plant Cell, 18: 2893-903 (2006)
Aspirin upregulates expression of urokinase type plasminogen activator receptor (uPAR) gene in human colon cancer cells through AP1.
Md Saha Jamaluddin (2006) Biochemical and biophysical research communications.348:618-27
Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin.
Suka, N, et al. (2001) Mol. Cell, 8: 473-9 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Yeast (S. cerevisiae)  WB, ChIP-seq, ChIP, DB Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H3 (Lys27) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance GuaranteedFor the full resolution version of the ChIP-seq data Click Here.
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Histone H3 acetylated on lysine 27.
Molecular Weight:
17 kDa
Epitope:
Lys27
Immunogen:
synthetic peptide corresponding to the sequence ARACKSA, in which ACK corresponds to acetyl lysine 27 of yeast histone H3
Modifications:
Acetylation
Isotype:
IgG
Background Information:
"Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. "
View All »
Species Reactivity:
Human
Vertebrates
Yeast (S. cerevisiae)
Application Notes:
ChIP-seq Analysis: Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 2 µl of anti-acetyl-Histone H3 (Lys27) antibody (cat# 07-360), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of fourteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 07-360 dataset showed 96% overlap with peaks identified in the ENCODE H3K27Ac BROAD Histone track for HeLa S3.
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Control:
Acid extracted proteins from HeLa cells treated with sodium butyrate
Quality Assurance:
routinely evaluated by immunoblot on acid extracts from sodium butyr -20℃