A-ACET-HIST H3 (K18) CHIP GRD 100

货号： 07-354      产品名称： A-ACET-HIST H3 (K18) CHIP GRD 100   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H3 (Lys18) Antibody
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REFERENCES
cAMP response element modulator (CREM)α suppresses IL-17F expression in T lymphocytes from patients with systemic lupus erythematosus (SLE).
Hedrich, Christian M, et al. (2011) The Journal of biological chemistry, (2011)
cAMP-responsive element modulator (CREM)α protein induces interleukin 17A expression and mediates epigenetic alterations at the interleukin-17A gene locus in patients with systemic lupus erythematosus.
Rauen, Thomas, et al. (2011) J. Biol. Chem., 286: 43437-46 (2011)
cAMP-responsive element modulator (CREM)α protein signaling mediates epigenetic remodeling of the human interleukin-2 gene: implications in systemic lupus erythematosus.
Hedrich, Christian M, et al. (2011) J. Biol. Chem., 286: 43429-36 (2011)
Regulation of TCR Vγ2 gene rearrangement by the helix-loop-helix protein, E2A.
Masatoshi Nozaki,Kousho Wakae,Nobuyuki Tamaki,Shuji Sakamoto,Kiyouhisa Ohnishi,Toshifumi Uejima,Nagahiro Minato,Itaru Yanagihara,Yasutoshi Agata (2011) International immunology.23
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Yeast (S. cerevisiae)  WB, DB, ChIP, ChIP-seq Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H3 (Lys18) Antibody
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For the full resolution version of the ChIP-seq data Click Here.
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Histone H3 acetylated on lysine 18.
Molecular Weight:
17 kDa
Immunogen:
KLH-conjugated, synthetic peptide (GKAPRAcKQLASK-C) corresponding to amino acids 13-23 of yeast Histone H3 acetylated on lysine 18with a C-terminal cysteine added for conjugation purposes
Modifications:
Acetylation
Background Information:
Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
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Species Reactivity:
Human
Vertebrates
Yeast (S. cerevisiae)
Species Reactivity Note:
Yeast, Human, Most common vertebrates
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Normal Rabbit Serum , or 2 µL of Anti-Acetyl-Histone H3 (Lys18)and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of Acetyl-Histone H3 (Lys18) associated DNA fragments was verified by qPCR using Control Primers specific for the human GAPDH promoter region as a positive locus, and β-globin primers as a negative locus. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
ChIP-Sequencing:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 2 μg of anti-acetyl-Histone H3 (Lys18) antibody (cat# 07-354), 20 µL Protein A/G beads , and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols w -20℃