A-ACET-HIST H3(K27) 100UL

货号： 07-360      产品名称： A-ACET-HIST H3(K27) 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H3 (Lys27) Antibody
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REFERENCES
Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila.
Kathryn Parker,Julia Maxson,Alissa Mooney,Emily A Wiley (2007) Eukaryotic cell.6
Arabidopsis GCN5, HD1, and TAF1/HAF2 interact to regulate histone acetylation required for light-responsive gene expression.
Benhamed, Moussa, et al. (2006) Plant Cell, 18: 2893-903 (2006)
Aspirin upregulates expression of urokinase type plasminogen activator receptor (uPAR) gene in human colon cancer cells through AP1.
Md Saha Jamaluddin (2006) Biochemical and biophysical research communications.348:618-27
Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin.
Suka, N, et al. (2001) Mol. Cell, 8: 473-9 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Yeast (S. cerevisiae)  WB, ChIP-seq, ChIP, DB Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H3 (Lys27) Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Histone H3 acetylated on lysine 27.
Molecular Weight:
17 kDa
Epitope:
Lys27
Immunogen:
synthetic peptide corresponding to the sequence ARACKSA, in which ACK corresponds to acetyl lysine 27 of yeast histone H3
Modifications:
Acetylation
Isotype:
IgG
Background Information:
"Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. "
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Species Reactivity:
Human
Vertebrates
Yeast (S. cerevisiae)
Application Notes:
ChIP-seq Analysis: Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 2 µl of anti-acetyl-Histone H3 (Lys27) antibody (cat# 07-360), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of fourteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. The highest 25% of peaks identified in the 07-360 dataset showed 96% overlap with peaks identified in the ENCODE H3K27Ac BROAD Histone track for HeLa S3.
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Control:
Acid extracted proteins from HeLa cells treated with sodium butyrate
Quality Assurance:
routinely evaluated by immunoblot on acid extracts from sodium butyr -20℃