A-ACET-HIST H3(K23), CHIP GRD 100UL

货号： 07-355      产品名称： A-ACET-HIST H3(K23), CHIP GRD 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H3 (Lys23)
Close
REFERENCES
Corepressor-directed preacetylation of histone H3 in promoter chromatin primes rapid transcriptional switching of cell-type-specific genes in yeast.
Alec M Desimone,Jeffrey D Laney (2010) Molecular and cellular biology.30
Highly specific antibodies determine histone acetylation site usage in yeast heterochromatin and euchromatin.
Suka, N, et al. (2001) Mol. Cell, 8: 473-9 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Yeast (S. cerevisiae)  WB, DB, ChIP Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H3 (Lys23) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Histone H3 acetylated on lysine 23. Does not recognize unacetylated recombinant histone H3
Molecular Weight:
17kDa
Immunogen:
Ovalbumin-conjugated, synthetic peptide (KQLASAcKAARK-C) corresponding to amino acids 18-27 of yeast histone H3 acetylated on lysine 23 with a C-terminal cysteine added for conjugation purposes
Modifications:
Acetylation
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
View All »
Species Reactivity:
Human
Vertebrates
Yeast (S. cerevisiae)
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit serum or 2 µL Anti-acetyl-Histone H3 (Lys23)and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of acetyl-Histone H3 (Lys23) associated DNA fragments were verified by qPCR using Control Primers specific for the human GAPDH promoter region as a positive locus, and MyoD primers as a negative locus. Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Acid extracts from sodium butyrate treated HeLa cells (Lane 1, Catalog # 17-305) and recombinant Histone H3 (Lane 2, Catalog # 14-494) were probed with Anti-acetyl-Histone H3 (Lys23) (1:100,000 dilution).
Arrow indicates acetyl histone H3 (~17 kDa)
Dot Blot:
Representative lot data.
40 ng and 4ng amounts of histone peptides with various modifications (see table 1) were transferred to PVDF membrane and probed with Anti-Acetyl-Histone H3 (Lys23) antibody (1:2000 dilution). Proteins were visualized using a goat anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system. Image from a 60 second exposure is shown.
View All »
Control:
Acid extracts from sodium butyrate treated HeLa cells
Quality Assurance:
routinely evaluated by immunoblot on acid extracts from sodium butyrate treated HeLa cells
Purification Method:
Antiserum
Presentation:
Rabbit antiserum containing 0.05% -20℃