A-ACET-HIST H4(K5), CHIP GRD 100UL

货号： 07-327      产品名称： A-ACET-HIST H4(K5), CHIP GRD 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H4 (Lys5)
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REFERENCES
HDA19 is required for the repression of salicylic acid biosynthesis and salicylic acid-mediated defense responses in Arabidopsis.
Sun-Mee Choi,Hae-Ryong Song,Soon-Ki Han,Muho Han,Chi-Yeol Kim,Jaejin Park,Yong-Hwan Lee,Jong-Seong Jeon,Yoo-Sun Noh,Bosl Noh (2012) The Plant journal : for cell and molecular biology.71
HBO1 is required for H3K14 acetylation and normal transcriptional activity during embryonic development.
Andrew J Kueh,Mathew P Dixon,Anne K Voss,Tim Thomas (2011) Molecular and cellular biology.31
Histone crosstalk between H3S10ph and H4K16ac generates a histone code that mediates transcription elongation.
Zippo, Alessio, et al. (2009) Cell, 138: 1122-36 (2009)
Activation of bovine somatic cell nuclear transfer embryos by PLCZ cRNA injection.
Pablo J Ross, Ramon M Rodriguez, Amy E Iager, Zeki Beyhan, Kai Wang, Neli P Ragina, Sook-Young Yoon, Rafael A Fissore, Jose B Cibelli, Pablo J Ross, Ramon M Rodriguez, Amy E Iager, Zeki Beyhan, Kai Wang, Neli P Ragina, Sook-Young Yoon, Rafael A Fissore, Jose B Cibelli (2009) Reproduction (Cambridge, England).137:427-37
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, DB, ICC, ChIP-seq, ChIP Rabbit Serum Polyclonal Antibody
Description:
Anti-acetyl-Histone H4 (Lys5) Antibody
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Specificity:
This antibody recognizes the N-terminus of Histone H4 acetylated at Lys5.
Molecular Weight:
~13 kDa observed
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminus of Histone H4 acetylated at Lys5.
Modifications:
Acetylation
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human based on 100% sequence homology. This antibody is expected to demonstrate broad species cross-reactivity.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected acetyl-Histone H4 in HeLa and A431 cells.
Dot Blot Specificity Analysis: A representative lot detected acetyl-histone H4 (Lys5) peptides, but not unacetylated-histone H4 (Lys4) peptides or other peptides corresponding to modified histones.
Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated acetyl-Histone H4 in HeLa S3 chromatin.
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Control:
HeLa acid extract
Quality Assurance:
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H4 in 10 µg of HeLa acid extract.
Purification Method:
Purified by immunodepletion
Presentation:
Immunodepleted rabbit serum with 0.05% soduim azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C -20℃