A-ACET-HIST H4(K16) 100UL

货号： 07-329      产品名称： A-ACET-HIST H4(K16) 100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-acetyl-Histone H4 (Lys16) Antibody
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REFERENCES
Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination.
Tang, Jiangbo, et al. (2013) Nat. Struct. Mol. Biol., 20: 317-25 (2013)
BCL6 controls neurogenesis through Sirt1-dependent epigenetic repression of selective Notch targets.
Tiberi, Luca, et al. (2012) Nat. Neurosci., (2012)
Sir3 and Epigenetic Inheritance of Silent Chromatin in Saccharomyces cerevisiae.
Tina Motwani,Minakshi Poddar,Scott G Holmes (2012) Molecular and cellular biology.32
FoxA1 and glucocorticoid receptor crosstalk via histone H4K16 acetylation at a hormone regulated enhancer.
Belikov S, Holmqvist PH, Astrand C, Wrange O (2012) Experimental cell research.318:61-74. Epub 2011 Oct 6.
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Yeast (S. cerevisiae)  WB, Mplex, PIA, DB, ChIP-seq, ChIP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-acetyl-Histone H4 (Lys16) Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
Histone H4 acetylated on lysine 16, but not histone H4 containing a lysine 16 to arginine substitution.
Molecular Weight:
10 kDa
Epitope:
Lys16
Immunogen:
Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.
Modifications:
Acetylation
Isotype:
IgG
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
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Species Reactivity:
Human
Vertebrates
Yeast (S. cerevisiae)
Species Reactivity Note:
Yeast and human. Broad species cross-reactivity is expected.
Application Notes:
Multiplexing:
This antibody specifically recognizes histone H4 acetylated on Lys16 by Luminex® assay.
Peptide Inhibition Assay:
This antibody peptide blocked on HeLa cell extracts.
Chromatin Immunoprecipitation
An independent laboratory has shown that this antibody preferentially immunopre-cipitates chromatin from wild type yeast, compared to yeast containing a histone H4 lysine 16 to arginine substitution.
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Control:
TSA treated NIH/3T3 cells.
HeLa acid extract
Quality Assurance:
Evaluated by western blot on acid extracts of HeLa cells.
Western Blot Analysis:
0.1 µg/mL of this antibody detected Histone H4 acetylated on Lys16 in 10 µg of HeLa Acid extract cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8° C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
UniProt Number:
P62805
Entrez Gene Number:
NM_175054
Gene Symbol:
HIST1H4A
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIST1H4B
H4FE
H4/M
H4/a
HI 2-8℃