ANTI-DIMETHYL-HIST H3

货号： 07-030      产品名称： ANTI-DIMETHYL-HIST H3   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-dimethyl-Histone H3 (Lys4)
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REFERENCES
Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.
Culver-Cochran, Ashley E and Chadwick, Brian P (2013) BMC Genomics, 14: 740 (2013)
The human GFI136N variant induces epigenetic changes at the Hoxa9 locus and accelerates K-RAS driven myeloproliferative disorder in mice.
Cyrus Khandanpour,Joseph Krongold,Judith Sch,Frederique Bouwman,Lothar Vassen,Marie-Claude Gaudreau,Riyan Chen,Fernando J Calero-Nieto,Evangelia Diamanti,Rebecca Hannah,Sara E Meyer,H Leighton Grimes,Bert A van der Reijden,Joop H Jansen,Chandrashekhar V Patel,Justine K Peeters,Bob L,Ulrich D,Bertie G,Tarik M (2012) Blood.120
The macrosatellite DXZ4 mediates CTCF-dependent long-range intrachromosomal interactions on the human inactive X chromosome.
Andrea H Horakova,Shawn C Moseley,Christine R McLaughlin,Deanna C Tremblay,Brian P Chadwick (2012) Human molecular genetics.21
Early-stage epigenetic modification during somatic cell reprogramming by Parp1 and Tet2.
Claudia A Doege,Keiichi Inoue,Toru Yamashita,David B Rhee,Skylar Travis,Ryousuke Fujita,Paolo Guarnieri,Govind Bhagat,William B Vanti,Alan Shih,Ross L Levine,Sara Nik,Emily I Chen,Asa Abeliovich (2012) Nature.488
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Species Reactivity Key Applications Host Format Antibody Type
H, T  DB, ICC, IF, WB, PIA, ChIP-seq, ChIP Rabbit Unpurified Polyclonal Antibody
Description:
Anti-dimethyl-Histone H3 (Lys4) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes dimethylated Histone H3 (Lys4), MW ~17 kDa. Specificity demonstrated by ELISA using methylated peptides.
Molecular Weight:
Approx. 17 kDa
Epitope:
dimethylated Histone H3 (Lys4)
Immunogen:
KLH conjugated synthetic peptide (ARTMe2KQTAR-GC) corresponding to amino acids 1-8 of human Histone H3.
Modifications:
Methylation
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Species Reactivity:
Human
Tetrahymena
Species Reactivity Note:
Human and tetrahymena. Broad species cross-reactivity is expected.
Application Notes:
Chromatin Immunoprecipitation:
5 μL of a previous lot immunoprecipitated methylated Histone H3 from 1 x 106 3T3/A31 cells.
Immunocytochemistry:
1:500 dilution of a previous lot showed positive immunostaining for methylated Histone H3 in L6 cells fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100.
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Control:
HeLa acid extract
Quality Assurance:
Evaluated by western blot on HeLa acid extract.
Western Blot Analysis: 1:2000-1:10,000 dilution of this antibody detected methylated Histone H3 in acid extracted proteins from HeLa cells.
1:2000-1:5000 dilution of a previous lot detected methylated Histone H3 in acid extracted proteins from HeLa and tetrahymena cells.
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Purification Method:
Unpurified
Presentation:
Rabbit polyclonal antiserum containing -20°C

ANTI-DIMETHYL-HIST H4 (LYS20)100UL

货号： 07-031      产品名称： ANTI-DIMETHYL-HIST H4 (LYS20)100UL   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-dimethyl-Histone H4 (Lys20) Antibody
Recommended Replacement for: 07-747
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REFERENCES
A cellular study of teosinte Zea mays subsp. parviglumis (Poaceae) caryopsis development showing several processes conserved in maize.
Marina Dermastia,Ales Kladnik,Jasna Dolenc Koce,Prem S Chourey (2009) American journal of botany.96
An in vitro investigation of herbs traditionally used for kidney and urinary system disorders: potential therapeutic and toxic effects.
Ken Wojcikowski,Hans Wohlmuth,David W Johnson,Margaret Rolfe,Glenda Gobe (2009) Nephrology (Carlton, Vic.).14
Species Reactivity Key Applications Host Format Antibody Type
H  IF, WB, Mplex, ICC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-dimethyl-Histone H4 (Lys20) Antibody
Replaces:
07-747
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
recognizes Histone H4 dimethylated on Lysine 20
Molecular Weight:
10kDa
Immunogen:
Peptide corresponding to amino acids 17-28 of human histone H4 (RHRdimKVLRDNIQG-C) with lysine 20 dimethylated.
Modifications:
Methylation
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot on acid-extracted HeLa cells
Purification Method:
Immunoaffinity chromatography
Presentation:
0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
Storage Conditions:
1 year at -20°C
UniProt Number:
P62805
Q6DRA9
Entrez Gene Number:
NM_175054
Gene Symbol:
HIST1H4A
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIST1H4B
H4FE
H4/M
H4/a
HIST1H4E
HIST4H4
HIST2H4A
H4FC
H4FI
H4/H
HIST1H4C
H4/G
HIST1H4K
H4FJ
H4FD
H4/I
H4/B
H4/D
H4/C
HIST1H4J
HIST1H4D
H4/E
View All »
Alternate Names:
H4K20me2
Histone H4 (di methyl K20)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunofluorescence
Western Blotting
Multiplexing
Immunocytochemistry
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
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UniProt Summary:
FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility t -20°C

供原装|ANTI-DIMETHYL-HIST H3(LYS9)

货号： 07-212      产品名称： 供原装|ANTI-DIMETHYL-HIST H3(LYS9)   品牌： Millipore 规格： EA 大量现货 科研实验

Anti-dimethyl-Histone H3 (Lys9) Antibody
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REFERENCES
IVMBIX-01294, an inhibitor of the histone methyltransferase EHMT2, disrupts histone H3 lysine 9 (H3K9) dimethylation in the cleavage-stage porcine embryo.
Ki-Eun Park,Christine M Johnson,Ryan A Cabot (2012) Reproduction, fertility, and development.24
MeCP2 binds to nucleosome free (linker DNA) regions and to H3K9/H3K27 methylated nucleosomes in the brain.
Anita A Thambirajah,Marlee K Ng,Lindsay J Frehlick,Andra Li,Jason J Serpa,Evgeniy V Petrotchenko,Begonia Silva-Moreno,Kristal K Missiaen,Christoph H Borchers,J Adam Hall,Ryan Mackie,Frank Lutz,Brent E Gowen,Michael Hendzel,Philippe T Georgel,Juan Ausió (2012) Nucleic acids research.40
The RNA helicase Rm62 cooperates with SU(VAR)3-9 to re-silence active transcription in Drosophila melanogaster.
Joern Boeke,Indira Bag,M Janaki Ramaiah,Irene Vetter,Elisabeth Kremmer,Manika Pal-Bhadra,Utpal Bhadra,Axel Imhof (2011) PloS one.6
Developmental role for ACF1-containing nucleosome remodellers in chromatin organisation.
Chioda M, Vengadasalam S, Kremmer E, Eberharter A, Becker PB (2010) Development.137:3513-22. Epub 2010 Sep 15.
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Species Reactivity Key Applications Host Format Antibody Type
H, Ch, Vrt, Yeast (S. cerevisiae)  ICC, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-dimethyl-Histone H3 (Lys9) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Methylated Histone H3 (Lys9). Specificity demonstrated by peptide inhibition assay using methylated and unmethylated peptides. Slight cross-reactivity to the trimethyl Lys27 Histone H3.
Molecular Weight:
17 kDa
Epitope:
a.a. 6-13
Immunogen:
KLH-conjugated synthetic peptide corresponding to amino acids 6-13 (TAR[dimethyl-K]STGG-C) of Histone H3 with C added to the Cterminus for conjugation. The immunizing sequence is conserved from Tetrahymena to human.
Modifications:
Methylation
Isotype:
IgG
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
View All »
Species Reactivity:
Human
Chicken
Vertebrates
Yeast (S. cerevisiae)
Species Reactivity Note:
Human. Expected to cross react with fission yeast and chicken.
Application Notes:
Not recommended for ChIP.
Control:
HeLa, NIH/3T3 or COS cell extracts.
Quality Assurance:
Routinely evaluated by Western Blot on HeLa acid extract and recombinant Histone H3
(cat# 14-411).
Western Blot Analysis:
1:500 dilution of this lot detected dimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.
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Purification Method:
Immunoaffinity chromatography
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 5 mg/mL BSA, 0.05% sodium azide before the addition of glycerol to 30%.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap 2-8度

ANTI-DIMETHYL-HIST H4 (ARG3)100UL

货号： 07-213      产品名称： ANTI-DIMETHYL-HIST H4 (ARG3)100UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-dimethyl-Histone H4 (Arg3) Antibody
Close
REFERENCES
Acute running stimulates hippocampal dopaminergic neurotransmission in rats, but has no influence on brain-derived neurotrophic factor.
Maaike Goekint,Inge Bos,Elsa Heyman,Romain Meeusen,Yvette Michotte,Sophie Sarre (2012) Journal of applied physiology (Bethesda, Md. : 1985).112
Friend of Prmt1, a novel chromatin target of protein arginine methyltransferases.
van Dijk TB, Gillemans N, Stein C, Fanis P, Demmers J, van de Corput M, Essers J, Grosveld F, Bauer UM, Philipsen S (2010) Molecular and cellular biology.30:260-72
USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.
Suming Huang,Xingguo Li,Timur M Yusufzai,Yi Qiu,Gary Felsenfeld (2007) Molecular and cellular biology.27
SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis.
Wang, Xin, et al. (2007) EMBO J., 26: 1934-41 (2007)
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt, Inv  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-dimethyl-Histone H4 (Arg3) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes methylated Histone H4 (Arg3), MW 10 kDa. Addition of 0.1% Tween®-20 in western blot buffers results in the detection of additional proteins at 17 kDa (possibly methylated Histone H2a), 34 kDa and 80 kDa. Specificity demonstrated by ELISA using methylated and unmethylated Histone H4 peptides. The antibody does not detect recombinant Histone H3 purified from E. coli.
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Molecular Weight:
10 kDa
Immunogen:
BSA-conjugated synthetic peptide (SG[me2R]GKGGKG-C) corresponding to amino acids 1-9 of Histone H4 with a C-terminal cysteine added to facilitate conjugation. The immunizing sequence is conserved from Tetrahymena to human.
Modifications:
Methylation
Isotype:
IgG
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ‘beads on a string’ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
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Species Reactivity:
Human
Vertebrates
Invertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected.
Application Notes:
Methylation/Western Blot Assay: Reported by an independent laboratory to detect Histone H4 methylated in vitro by recombinant PRMT1 and detected by western blot analysis.
Control:
Acid extracted proteins from HeLa cells.
Quality Assurance:
Western Blot Analysis: A 1:500-1:2000 dilution of this antibody detected methylated Histone H4 in acid-extracted proteins from HeLa cells.
Purification Method:
Immunoaffinity chromatography
Presentation:
Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, 5 mg/mL BSA and 30% glycerol.
Storage Conditions:
Stable for 1 year at from date of receipt.
UniProt Number:
P62805
Entrez Gene Number:
NP_778224
Gene Symbol:
HIST1H4A
H4/J
HIST1H4F
HIST1H4L
H4FN
H4FA
H4FH
HIST1H4H
H4F2
H4FM
H4/A
H4FK
H4FG
HIST2H4
H4FB
H4/K
HIST1H4I
H4/N
HIS -20℃